1CEV,1T5G,2RLA,1WVA,2PLL,3MMR,4CEV,2EF4,2EIV,1D3V,1R1O,1T4S,3E6K,3THH


Conserved Protein Domain Family
Arginase

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cd09989: Arginase 
Click on image for an interactive view with Cn3D
Arginase family
This family includes arginase, also known as arginase-like amidino hydrolase family, and related proteins. Arginase is a binuclear Mn-dependent metalloenzyme and catalyzes hydrolysis of L-arginine to L-ornithine and urea (Arg, EC 3.5.3.1), the reaction being the fifth and final step in the urea cycle, providing the path for the disposal of nitrogenous compounds. Arginase controls cellular levels of arginine and ornithine which are involved in protein biosynthesis, and in production of creatine, polyamines, proline and nitric acid. In vertebrates, at least two isozymes have been identified: type I (ARG1) cytoplasmic or hepatic liver-type arginase and type II (ARG2) mitochondrial or non-hepatic arginase. Point mutations in human arginase ARG1 gene lead to hyperargininemia with consequent mental disorders, retarded development and early death. Hyperargininemia is associated with a several-fold increase in the activity of the mitochondrial arginase (ARG2), causing persistent ureagenesis in patients. ARG2 overexpression plays a critical role in the pathophysiology of cholesterol mediated endothelial dysfunction. Thus, arginase is a therapeutic target to treat asthma, erectile dysfunction, atherosclerosis and cancer.
Statistics
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PSSM-Id: 212515
Aligned: 135 rows
Threshold Bit Score: 245.482
Created: 16-Dec-2010
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Active site is composed of the metal binding (Mn), water and substrate binding (arginine) site.
  • Comment:Two Mn2+ ions are located in the active site. A The bridging water symmetrically positioned between them is responsible for hydrolytic attack on the guanidine group of the substrate.
  • Structure:1T5G: Rattus norvegicus arginase binds two Mn ions, water molecule and arginine; contacts at 4A.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                       
1CEV_A        4 ISIIGVPMDLGQtRRGVDMGPSAMRYAGVierle----rlhydIEDLGDIpigkaerlheqg---dsrlrnlkavaEANE 76  Bacillus caldovelox
1T5G_A        3 IEIIGAPFSKGCpRGGVEKGPAALRKAGLveklk----eteynVRDHGDLafvdvpndspf-----qivknprsvgKANE 73  Norway rat
AEV31723      5 LQFLSIMSELGAgTRGASLGFPALEIASLkydpeffpkysikeVETDNNLvfkkktlkf---------gkrldgiaRMYR 75  Owenweeksia hong...
ZP_03702315   4 IRILKNRSDIGAgTRGSDMGIDALEIAAInsghdffnrhpfidIPTHNETvydkvkntt---------akrinfvaAQCE 74  Flavobacteria ba...
ZP_07083525   5 IELIKNRSDIGAgTRGSDLGIDAIEIAAInkgslffkkypfvdVQTRNDSvyendfnpf---------gkhikqvyQQCK 75  Sphingobacterium...
Q1Q9T2        8 IRIVEVYSDIAGyKKGASLGIDALKASSKelnadyfeklqfdsIEDDSNNegesqfah----------akyadviePIIS 77  Psychrobacter cr...
ZP_07744723   8 EHAIIVECELGGpHNGASMAGRAFFSQFEqrtnvretittpirKVRQSNTpkahy----------------lsdinHCLR 71  Vibrio sp. SP2010
ZP_04580714   2 IEILGIASDIAGgKLGSAKGVALLCEYMRekn--------laiLHDKNYLsldssiippaqk--ddytkhidvlydFFKD 71  Helicobacter bil...
ADN80649      1 MILVGLEAELGAsKRGTDKGVRRLREALSe------------tHGDVIKGmqtiiqercvlykefryaknfedyylFCKE 68  Helicobacter pyl...
CBY83234      1 MVLVNLVAELGAaKHGSRVGVEKLKSRLIecy--------pqiTEHMALIrqrqcvlerff----ryaknfedyyfFCKD 68  Helicobacter fel...
Feature 1                               #                        # # # #          ##            
1CEV_A       77 KLAAAVDQVVQRg--rFPLVLGGDHSIAIGTLAGvakh--yerLGVIWYDAHGDVNTaetspSGNIHGMPLAASLgfghp 152 Bacillus caldovelox
1T5G_A       74 QLAAVVAETQKNg--tISVVLGGDHSMAIGSISGharv--hpdLAVIWVDAHTDINTplttsSGNLAGQPVAFLLkelkg 149 Norway rat
AEV31723     76 NIQDEVAATFNDk--kFPVVFSGDHSNAGGTIAGikaayptkrLGVIWVDAHGDLHSpytspTGNVHGMPLATALhednl 153 Owenweeksia hong...
ZP_03702315  75 RLKLATKQALKEg--fFPLILSGDHSSALGSLAGiksnfpektLGVVWIDAHADLHTpfsspSGNVHGMPLAAALghdna 152 Flavobacteria ba...
ZP_07083525  76 QVASIVEDSILKe--nFPLVFSGDHSSAIGTISGikaalpdkrLGVIWIDAHADIHSpyttpSGNLHGMPIAAVLqkdnl 153 Sphingobacterium...
Q1Q9T2       78 KLAHKIKDIRNEn--kFPIVLAGDHSSCAGTMHGlkmahpdaeIGVVYIDAHADFHSpytsgSGNMHGMPLAMACaidnl 155 Psychrobacter cr...
ZP_07744723  72 DISEAVVKHYKQspesRLLVVAGDHSTACGTLAGlkrahpkskIGTIWIDAHADIHSpytspSGNVHGMPVAAAAgddhi 151 Vibrio sp. SP2010
ZP_04580714  72 KVAKRAEEIICEn--nFPFIISGDHSSALAVAQGiqnalpderIGIIWIDAHADIHTpfstfSGNLHGMPLAAIIghkap 149 Helicobacter bil...
ADN80649     69 NLIPCMKEVFEKk--eFPLILSSEHANMFGIFQAfrsvhkdkkIGILYLDAHADIHTaydsdTKHIHGMPLGMVLnrvhs 146 Helicobacter pyl...
CBY83234     69 SLIPTMHAIFKRg--sFPLILSAEHSGAFGIAQAlrsvhpdkkIGILYLDAHADIHTaydsdSGNLHGMPLGMVLnrihs 146 Helicobacter fel...
Feature 1                                                #  #                                   
1CEV_A      153 altq----------------iggyspkikPEHVVLIGVRSLDEGEKKFIRekGIKIYTMHEVDRLgMTRVMEETIAYLke 216 Bacillus caldovelox
1T5G_A      150 kfpdvp-------------gfswvtpaisAKDIVYIGLRDVDPGEHYIIKtlGIKYFSMTEVDKLgIGKVMEETFSYLlg 216 Norway rat
AEV31723    154 enknndpqeet---iwywnemkgsnqrvkHSDLFFVGLRDLEQPEINLMTkhNIPNVTVDELREMgVKAVAEKALIYLkd 230 Owenweeksia hong...
ZP_03702315 153 dqkinnvshetiadwqqmknmgsvspilkPEHLIYFGVRDTEAQEDAIIErfGIRNYGVHEMRHRsFEICIAEALEQLse 232 Flavobacteria ba...
ZP_07083525 154 lsrinevdshtqtyweklkhvgtaepklmAEDLVYFGVRDTEEPEDMLIErlGIKNYQVGEVRTKkVEQCVKEVFDQLkn 233 Sphingobacterium...
Q1Q9T2      156 ecqrnelseketeyweklkfmassepsikPENVVFCAVRDYQEEEMDLIRrhKIPLYSVAEITQKgISSTVEEIFKKLey 235 Psychrobacter cr...
ZP_07744723 152 dfkikeldeet--iklweatkdicqnsieYNNLIYIGIRDLEKAEWDVIAknNINHYSVDAIENRnCSEVCSEIFLYLeh 229 Vibrio sp. SP2010
ZP_04580714 150 nkntltqsqe----esweklmniskdsiqPENIVYCGIRSLEKAEQDVIDskNICVFSVESLQKG-RADCIEKMKEVLtq 224 Helicobacter bil...
ADN80649    147 gvnhmneseekawqklcslglekggleidPKCLVYFGVRSTEQSERDVIKelEIPLFSVDAIREN-MHEVVQKTKELLka 225 Helicobacter pyl...
CBY83234    147 gqnvlndqekhyweslcalgledgaldfdPKHLIYFGVRSTEASEREMIKkyHIPLFSIEAIRAD-MPGVVQQTQALLee 225 Helicobacter fel...
Feature 1                 # #                                             #                     
1CEV_A      217 -rtDGVHLSLDLDGLDPS-DAPGVGTPVIGGLTYRESHLAMEMLAe--aQIITSAEFVEVNPILDer-----nKTASVAV 287 Bacillus caldovelox
1T5G_A      217 rkkRPIHLSFDVDGLDPV-FTPATGTPVVGGLSYREGLYITEEIYk--tGLLSGLDIMEVNPTLGktpe-evtRTVNTAV 292 Norway rat
AEV31723    231 --cDIIYISFDVDSMDPS-VSRGTGTPVEHGLTEDEARDLLLHLVe--dERLCCFETTEVNPLLDdk----gnSMAEAAF 301 Owenweeksia hong...
ZP_03702315 233 --vDMIYITFDVDALDCDlISHGTGTPVSKGFDVDEVVRIIKGIQt--tEKVVALEVCEINPLLDhk----gnRMAEAAF 304 Flavobacteria ba...
ZP_07083525 234 --cDILYVSFDVDSMDSEkISEGTGTPVPEGFFPFEVTLLLQELIr--sEKVVCMEVVEVNPLLDhh----gnRMAEVTF 305 Sphingobacterium...
Q1Q9T2      236 --cDHIYVSFDVDSVDPL-YVPGTGTPAMNGLSYEQAMQLNIELIk--nKKVCCWEMAEINPLYNnskd-dstRIFKILE 309 Psychrobacter cr...
ZP_07744723 230 --cDIIYISFDIDALDKK-YVPGTGTPVKDGLSLEKTQELINKLCq--nDKVKALEVVEINPLLDsy-----nETASRIT 299 Vibrio sp. SP2010
ZP_04580714 225 --sTKIYVSVDIDVLDCS-EFQSTGCNESNGLYMQELIMLTKDIIesfsHKIIAFELSEFNPTLGntkeqdkaAVCNFLH 301 Helicobacter bil...
ADN80649    226 --vDIIYLSLDLDIMDGK-FFTSTGVRENNGLSFDELRQLLGLLLenfkDRLGAVEVTEYNPTVStkh---tdEEEKQVL 299 Helicobacter pyl...
CBY83234    226 --vDLVYLSLDVDVLDGK-IFSSTGVRENNGLQPQELQALLDSLLrafgERLACVEITEYNPELWng----eeEDEKVVW 298 Helicobacter fel...
Feature 1             
1CEV_A      288 ALMGSL 293 Bacillus caldovelox
1T5G_A      293 ALTLSA 298 Norway rat
AEV31723    302 RVIKPV 307 Owenweeksia hongkongensis DSM 17368
ZP_03702315 305 EVLETL 310 Flavobacteria bacterium MS024-2A
ZP_07083525 306 DILEQV 311 Sphingobacterium spiritivorum ATCC 33861
Q1Q9T2      310 KVTQSL 315 Psychrobacter cryohalolentis K5
ZP_07744723 300 NMLAKA 305 Vibrio sp. SP2010
ZP_04580714 302 EVIESL 307 Helicobacter bilis ATCC 43879
ADN80649    300 EILDLI 305 Helicobacter pylori 908
CBY83234    299 GLLTRV 304 Helicobacter felis ATCC 49179

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