1QU5,1DMZ


Conserved Protein Domain Family
FHA_RAD53-like_rpt2

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cd22690: FHA_RAD53-like_rpt2 
Click on image for an interactive view with Cn3D
second forkhead associated (FHA) domain found in Saccharomyces cerevisiae Serine/threonine-protein kinase RAD53 and similar proteins
RAD53, also called CHEK2 homolog, or serine-protein kinase 1 (Spk1), is a nuclear protein kinase that phosphorylates proteins on serine, threonine, and tyrosine. It controls S-phase checkpoint as well as G1 and G2 DNA damage checkpoints and prevents entry into anaphase and mitotic exit after DNA damage via regulation of the Polo kinase CDC5. It may be involved in the phosphorylation of RPH1. RAD53 contains two FHA domains. This model corresponds to the second one. The FHA domain is a small phosphopeptide recognition module.
Statistics
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PSSM-Id: 438742
Aligned: 15 rows
Threshold Bit Score: 120.86
Created: 4-Sep-2020
Updated: 17-Oct-2022
Structure
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Program:
Drawing:
Aligned Rows:
 
putative
Conserved site includes 9 residues -Click on image for an interactive view with Cn3D
Feature 1:putative phosphopeptide binding site [polypeptide binding site]
Evidence:
  • Comment:the C-terminal Rad53 FHA domain contacts phosphate through the side chains of Arg605, Arg617 and Arg620.
  • Comment: based on structures of other FHA domains with bound phosphopeptide
  • Comment:GR at the C terminus of strand beta3, SRxH just preceding beta5, and motif SxNG in the beta6-beta7 turn indicate a canonical mode of pThr recognition.
  • Comment:Conserved residues are involved in binding directly to the ligand backbone and phosphate group. Non-conserved residues may determine binding specificity.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                ##                    # ## #                         
1QU5_A     29 FLTLKPLPDsiiQESLEIQqgvnPFFIGRSED--CNCKIEDn-------RLSRVHCFIFKKrhavgksmyespaqglDDI 99  baker's yeast
1DMZ_A      5 FLTLKPLPDsiiQESLEIQqgvnPFFIGRSED--CNCKIEDn-------RLSRVHCFIFKKrhavgksmyespaqglDDI 75  baker's yeast
P22216    577 FLTLKPLPDsiiQESLEIQqgvnPFFIGRSED--CNCKIEDn-------RLSRVHCFIFKKrhavgksmyespaqglDDI 647 Saccharomyces cere...
CDO55299  548 WLALNTLDNsspHPGVFLTs--sRTVFGRVATseVDIVVNFp-------QVSKKHCIIYVDnsg-----------ekPTV 607 Geotrichum candidum
ODQ76604  572 WLLLETMKDsipYKDIMIFr--eQAWFGRARK--CDIQLTDs-------RTSKFHCYLRRKrnshgggd-aghkrdlDAI 639 Lipomyces starkeyi...
RUS13865   60 WGRLNTINTe--YKTAWLTd--dITFFGRQQD--CHGELTYvssqpdhkKISAHHCAIIKKd---------------DDA 118 Endogone sp. FLAS-...
XP_648865   8 WGVLKSQNEa--YPDILLNk--nVTRIGRGYQ--SFLETKT--------VISFNHFTVKKDfp------------siGHV 61  Entamoeba histolyt...
Q54VI1     26 WGILKSLNPs--YPDISLVe--nVITFGRLKD--STVHYNDk-------SISGSHCKITREsndd---------dgvVIA 83  Dictyostelium disc...
P34101     96 WGKLISLNPt--YPTIEIRq--dSIILGRSKGv-CNYTFTSp-------TVSGKHCKIYRDptv----------ksrNVA 153 Dictyostelium disc...
EMS17278    5 WGVLCSLGR---VPSLLLIe--nETMIGRSNKf-CCLSKEK--------SISHKHCIITRVttpn---------enlSHC 61  Entamoeba histolyt...
Feature 1          # ##                                                     
1QU5_A    100 WYCHTGTNVSYLNNNRMIqgTKFLLQDGDEIKIIWDKn--------------nKFVIGFKVE 147 baker's yeast
1DMZ_A     76 WYCHTGTNVSYLNNNRMIqgTKFLLQDGDEIKIIWDKn--------------nKFVIGFKVE 123 baker's yeast
P22216    648 WYCHTGTNVSYLNNNRMIqgTKFLLQDGDEIKIIWDKn--------------nKFVIGFKVE 695 Saccharomyces cerevisiae S288C
CDO55299  608 WLIDTSSNGCYVNNRKVGkgNQAILQHNDKVYLFWDRh--------------sRKFLGFVAN 655 Geotrichum candidum
ODQ76604  640 YLSDFSTNGCFVNNQRVGsgMQATLKNGDEIAMFKER----------------NEKLAFKVQ 685 Lipomyces starkeyi NRRL Y-11557
RUS13865  119 FIQDLGMNGTFLNGDRLPprVLYMLKDGDEIVLSYADdqsdlrqkgacdrhgrLVHIGYIFQ 180 Endogone sp. FLAS-F59071
XP_648865  62 YLLDSSTNGTFINHSRCN--ESSEIYCYDEITMCQPLs---------------SVAISYMFI 106 Entamoeba histolytica HM-1:IMSS
Q54VI1     84 FIYDNSTNGTFIDNIKVGkgSRCLLASGQEISLTPQKq---------------LEKIAYIFQ 130 Dictyostelium discoideum
P34101    154 FVDDTSTNGTFINNEVIGkgSKILIENGCEISVIPKKg---------------SEKISFIYQ 200 Dictyostelium discoideum
EMS17278   62 FITDNSINGTFINRKYLGkgVQEEIHCFDTLTLLDPGl---------------KEHISYVFI 108 Entamoeba histolytica HM-3:IMSS

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