1UAZ,1E0P,3VHZ,4FBZ


Conserved Protein Domain Family
7tm_bacteriorhodopsin

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cd15244: 7tm_bacteriorhodopsin 
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light-driven outward proton pump bacteriorhodopsin, member of the seven-transmembrane GPCR superfamily
Bacteriorhodopsin (BR) serves as a light-driven retinal-binding outward proton pump, generating an outside positive membrane potential and thus creating an inwardly directed proton motive force (PMF) necessary for ATP synthesis. BR belongs to the microbial rhodopsin family, also known as type I rhodopsins, comprising light-driven inward chloride pump halorhodopsin (HR), light-gated cation channel channelrhodopsin (ChR), light-sensor activating transmembrane transducer protein sensory rhodopsin II (SRII), light-sensor activating soluble transducer protein Anabaena sensory rhodopsin (ASR), and other light-driven proton pumps such as blue-light absorbing and green-light absorbing proteorhodopsins, among others. They have been found in various single-celled microorganisms from all three domains of life, including halophile archaea, gamma-proteobacteria, cyanobacteria, fungi, and green algae. While microbial (type 1) and animal (type 2) rhodopsins have no sequence similarity with each other, they share a common architecture consisting of seven-transmembrane alpha-helices (TM) connected by extracellular loops and intracellular loops. Both types of rhodopsins consist of opsin and a covalently attached retinal (the aldehyde of vitamin A), a photoreactive chromophore, via a protonated Schiff base linkage to an amino group of lysine in the middle of the seventh transmembrane helix (TM7). Upon the absorption of light, microbial rhodopsins undergo light-induced photoisomerization of all-trans retinal into the 13-cis isomer, whereas the photoisomerization of 11-cis retinal to all-trans isomer occurs in the animal rhodopsins. While animal visual rhodopsins are activated by light to catalyze GDP/GTP exchange in the alpha subunit of the retinal G protein transducin (Gt), microbial rhodopsins do not activate G proteins, but instead can function as light-dependent ion pumps, cation channels, and sensors.
Statistics
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PSSM-Id: 320372
Aligned: 9 rows
Threshold Bit Score: 236.13
Created: 16-Feb-2014
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:ligand binding site [chemical binding site]
Evidence:
  • Structure:1UAZ: Halorubrum chaoviator archaerhodopsin binds retinal, contacts at 4A.
  • Structure:1E0P: Halobacterium salinarum bacteriorhodopsin binds retinal, contacts at 4A.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                     
1UAZ_A     13 RPETLWLGIGTLLMLIGTFYFIVKGWGVTDkeaREYYSITILVPGIASAAYLSMFFgigltevqv-gsemldiyYARYAD 91  Halobacterium sp.
1E0P_A      3 RPEWIWLALGTALMGLGTLYFLVKGMGVSDpdaKKFYAITTLVPAIAFTMYLSMLLgygltmvpf-ggeqnpiyWARYAD 81  Halobacterium sali...
3VHZ_A     20 RPEWIWLALGTALMGLGTLYFLVKGMGVSDpdaKKFYAITTLVPAIAFTMYLSMLLgygltmvpf-ggeqnpiyWARYAD 98  Halobacterium sp. ...
4FBZ_A      6 GPESIWLWIGTIGMTLGTLYFVGRGRGVRDrkmQEFYIITTFITTIAAAMYFAMATgfgvtevvv-gdealtiyWARYAD 84  Haloterrigena ther...
Q53496     12 GGESIFLWVGTAGMFLGMLYFIARGWSVSDqrrQKFYIATIMIAAIAFVNYLSMALgfgvttiel-ggeeraiyWARYTD 90  Haloarcula sp. ARG-2
YP_656804   5 GSEATWLWIGTIGMVLGTVYFAVRGRGSTDpeqQTYYIITTLIPAIAAAAYLAMATglgvismpirgtevidiyWARYAD 84  Haloquadratum wals...
AAV46888   12 EGEGIWLALGTVGMLLGMVYFMAKGWDVQDpeqEEFYVITILIAGIAASSYLSMFFgfgltevelvngrvidvyWARYAD 91  Haloarcula marismo...
ABG04982    2 EALWLWIGFVGMLLGTLYFAFLLTNAPEGT---RYFFVITATITGIAAIAYLVMATgsgstvl----pdgrefyWARYID 74  Rubrobacter xylano...
EFH82634    3 SATVTVLWVTSLLMILCTLVFTYRSFRARTe-iKHFYYLTALITLIAATLYMTMASgyggigq-----ngrvilFGRYID 76  Ktedonobacter race...
Feature 1     #  ##                                #   #                     #  ##  #         
1UAZ_A     92 WLFTTPLLLLDLALLAKvd-----rVSIGTLVGVDALMIVTGLVGAlsht------plaRYTWWLFSTICMIVVLYFLAT 160 Halobacterium sp.
1E0P_A     82 WLFTTPLLLLDLALLVDad-----qGTILALVGADGIMIGTGLVGAltkv------ysyRFVWWAISTAAMLYILYVLFF 150 Halobacterium sali...
3VHZ_A     99 WLFTTPLALLDLALLVDad-----qGTILALVGADGIMIGTGLVGAltkv------ysyRFVWWAISTAAMLYILYVLFF 167 Halobacterium sp. ...
4FBZ_A     85 WLFTTPLLLLDLGLLAGan-----rNTIATLIGLDVFMIGTGMIAAfaat------pgtRIAWWGISTGALLALLYVLVG 153 Haloterrigena ther...
Q53496     91 WLFTTPLLLYDLALLAGad-----rNTIYSLVGLDVLMIGTGALATlsagsgvlpagaeRLVWWGISTGFLLVLLYFLFS 165 Haloarcula sp. ARG-2
YP_656804  85 WLLTTPLLIIDLALVAGar-----kQTLYKLIIIDAIMILGGLAGSmmqq-----gaviRIVWWAVSTAAFIILLYYLLG 154 Haloquadratum wals...
AAV46888   92 WLFTTPLLLLDIGLLAGas-----nRDMASLITIDAFMIVTGLAATlmkv------pvaRYAFWTISTIAMLFVLYYLVV 160 Haloarcula marismo...
ABG04982   75 WVITTPLLLLDLCLLALadpr-rnvTFIASLIALDVVMILTGLWAGatvn------vagRAILFIISTAAFIGVLYLLVS 147 Rubrobacter xylano...
EFH82634   77 WVITTPLLLMNLALIALprnfpsrfAVIGTMIAADVYMIVSGLGASlir-------snfRWAFFAVSCAGFLAVLYFIVV 149 Ktedonobacter race...
Feature 1                                #  ##  #                       #  ##           
1UAZ_A    161 SLRAaakergPEVASTFNTLTALVLVLWTAYPILWIIGtegag-vvglGIETLLFMVLDVTAKVGFGFILLRSR 233 Halobacterium sp.
1E0P_A    151 GFTSkaesmrPEVASTFKVLRNVTVVLWSAYPVVWLIGsegag-ivplNIETLLFMVLDVSAKVGFGLILLRSR 223 Halobacterium salinarum
3VHZ_A    168 GFTSkaesmrPEVASTFKVLRNVTVVLWSAYPVVWLIGsegag-ivplNIETLLFMVLDVSAKVGFGLILLRSR 240 Halobacterium sp. NRC-1
4FBZ_A    154 TLSKdargqsPEVASLFGRLRNLVIVLWLLYPVVWILGtegtfgilplYWETAAFMVLDLSAKVGFGVVLLRSR 227 Haloterrigena thermotole...
Q53496    166 NLTDraselsGDLQSKFSTLRNLVLVLWLVYPVLWLVGteglg-lvglPIETAAFMVLDLTAKIGFGIILLQSH 238 Haloarcula sp. ARG-2
YP_656804 155 ELSErarsrsAETGIVFNRLRNITLGLWALYPIVWILGtgggfgiiavTTEIMLYVMLDIGTKIGFGAVLLESQ 228 Haloquadratum walsbyi DS...
AAV46888  161 VVGEaasdasEEAQSTFNVLRNIILVAWAIYPVAWLVGteglg-lvglFGETLLFMILDLTAKIGFGFILLRSR 233 Haloarcula marismortui A...
ABG04982  148 RLFAeasrrtPAVAQIFRTLAVLTIVLWICYPIVWLIGtegfg-avslSVEVFLFMVLDLLAKVGFGLLLLSSR 220 Rubrobacter xylanophilus
EFH82634  150 KLTPeanvrsEPVQRHYSTLSIMLIALWVCYPIVWILGtegfg-vislLPEVVLYAVLDILAKGAFGFVLLSKP 222 Ktedonobacter racemifer

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