1ZYD,1ZY4


Conserved Protein Domain Family
STKc_EIF2AK4_GCN2_rpt2

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cd14046: STKc_EIF2AK4_GCN2_rpt2 
Click on image for an interactive view with Cn3D
Catalytic domain, repeat 2, of the Serine/Threonine kinase, eukaryotic translation Initiation Factor 2-Alpha Kinase 4 or General Control Non-derepressible-2
STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. GCN2 (or EIF2AK4) is activated by amino acid or serum starvation and UV irradiation. It induces GCN4, a transcriptional activator of amino acid biosynthetic genes, leading to increased production of amino acids under amino acid-deficient conditions. In serum-starved cells, GCN2 activation induces translation of the stress-responsive transcription factor ATF4, while under UV stress, GCN2 triggers transcriptional rescue via NF-kB signaling. GCN2 contains an N-terminal RWD, a degenerate kinase-like (repeat 1), the catalytic kinase (repeat 2), a histidyl-tRNA synthetase (HisRS)-like, and a C-terminal ribosome-binding and dimerization (RB/DD) domains. Its kinase domain is activated via conformational changes as a result of the binding of uncharged tRNA to the HisRS-like domain. EIF2AKs phosphorylate the alpha subunit of eIF-2, resulting in the overall downregulation of protein synthesis. The GCN2 subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.
Statistics
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PSSM-Id: 270948
Aligned: 11 rows
Threshold Bit Score: 296.973
Created: 10-Apr-2007
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Structure:1ZYD_B; Saccharomyces cerevisiae GCN2 binds Mg2-ATP; contacts at 4A.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                   ##      #            # #                              #            
1ZYD_B       1 SLRYASDFEEIAVLGQGAFGQVVKARNalDSRYYAIKKIRHTee---klSTILSEVMLLASLNHQYVVRYYAAWLERRNf 77   baker's yeast
1ZY4_A       1 SLRYASDFEEIAVLGQGAFGQVVKARNalDSRYYAIKKIRHTee---klSTILSEVMLLASLNHQYVVRYYAAWLERRNf 77   baker's yeast
Q9HGN1     549 VSRYETDFEELEFLGRGGFGEVVKVKNriDGRFYAVKKLVLLsdd-kenSRILREVMTLSRLHHEHVVRYYTAWVETEAn 627  Schizosaccharomy...
Q9LX30     418 SSRYLNDFEELKPLGQGGFGHVVLCKNklDGRQYAVKKIRLKdkeipvnSRIVREVATLSRLQHQHVVRYYQAWFETGVv 497  thale cress
CAG10683   620 FSRYFNEFEELQLLGKGAFGAVIKVQNklDGCYYAVKRIQVNpas-khfRRIKGEVTLLSRLNHENIVRYYNAWIERHEt 698  Tetraodon nigrov...
AAC13490   516 QSRLRTEFEVLMYLGKGAFGDVLKVRNilDNREYAIKRIPLParsrqlyKKMTREVELLSRLNHENVVRYFNSWIESVDd 595  fruit fly
EAL17471   497 LSRYRADFEEVEFLGKGGFGEVVKARNklDGRFYAIKKVKLRped--neQKVYREVNNLSRVNHQHIVRYYGCWLEDANp 574  Cryptococcus neo...
XP_643344  487 QSRYHSDFEEIQLLGRGGFGQVVKVRNklDGRYYAIKKIKLDsnq-slnRRILREVITLSRLHHQHVVRYYQAWIESAEs 565  Dictyostelium di...
EAS06885   545 KSRYLTDFEEIQVLGQGAFGQVVKVRNklDGRYYAIKKIKIRkgq--niEKLMREVTLLSRLHHRHVLRYYQAWLEESNq 622  Tetrahymena ther...
BAE61949   575 FSRYYQDFDEAGRLGKGGFGMVVKARNklDGRLYAVKKITQRsaa--alKDTLSEIMLLSRLNHPYVVRYYTAWIEEDYd 652  Aspergillus oryzae
XP_421203  595 FSRYYNEFEELKLLGKGAFGAVIKVRNklDGCYYAVKRIRINtas-kqfRRIKGEVTLLSRLNHENIVRYYNAWIEKHEs 673  chicken
Feature 1                                                                                      
1ZYD_B      78 vkpmta-------------------------------------------------------------------------- 83   baker's yeast
1ZY4_A      78 vkpmta-------------------------------------------------------------------------- 83   baker's yeast
Q9HGN1     628 dtvteiissdseslsqslnmavdfrqssslpadklssldihfeddyns-------------------------------- 675  Schizosaccharomy...
Q9LX30     498 dpfaganwgsktagssmfs------------------------------------------------------------- 516  thale cress
CAG10683   699 pttgvlsstessepqstpdkspqgkkppqrlnelglpdnvedaappptlsssvewstsiersssak-------------- 764  Tetraodon nigrov...
AAC13490   596 adaaemdkllggewsqsqqdlsvkpakspqlgptleededeedssssmw------------------------------- 644  fruit fly
EAL17471   575 pdltptledsvhtastagtgatcsteedifavnfddfslsrrdqsrsasfprirftnsngdddddsdedds--------- 645  Cryptococcus neo...
XP_643344  566 lsltndnsddddddddefesgseseesqsesesesseseesseseessesseseeseeseysddsefeseenndfdqsfs 645  Dictyostelium di...
EAS06885   623 seeeesmgsqmsdqdddngelsddryryrsnnfssryqdn---------------------------------------- 662  Tetrahymena ther...
BAE61949   653 fvdeeavsstegdpfasqgsegsqdsqgygystggldfisssgypkief------------------------------- 701  Aspergillus oryzae
XP_421203  674 pvpivsstetsedkrtptkagliltaeetndveanapppvltssvewstscersssnkfsgadqe--------------- 738  chicken
Feature 1                                                                                      
1ZYD_B         --------------------------------------------------------------------------------      baker's yeast
1ZY4_A         --------------------------------------------------------------------------------      baker's yeast
Q9HGN1     676 -------------------------------------------------------------------------------s 676  Schizosaccharomy...
Q9LX30         --------------------------------------------------------------------------------      thale cress
CAG10683   765 ------------------------------------------------------------csgnqssdeedddededvfg 784  Tetraodon nigrov...
AAC13490   645 ------------------------------------------------------------------------------ng 646  fruit fly
EAL17471   646 --------------------------------------------------------seeddtesdtesdssaatadpsep 669  Cryptococcus neo...
XP_643344  646 evflfqngnkrslefdltddsysflhsdcgfllevfdlnnkiggnntrsmgssnqhlqqqqqqnqsqqqkkqpqqnqsqq 725  Dictyostelium di...
EAS06885       --------------------------------------------------------------------------------      Tetrahymena ther...
BAE61949   702 ------------------------------------------------------------------------------gs 703  Aspergillus oryzae
XP_421203  739 --------------------------------------------------------------ssddddddgdgvfshsfl 756  chicken
Feature 1                                                         ####                         
1ZYD_B      84 -----------------------------------------vkkkstLFIQMEYCEnrTLYDLIHSEnlnqQRDEYWRLF 122  baker's yeast
1ZY4_A      84 -----------------------------------------vkkkstLFIQMEYCEngTLYDLIHSEnlnqQRDEYWRLF 122  baker's yeast
Q9HGN1     677 adeedpeasdisfqysntsdkegssdkdssieeassvktqenglnatLYIQMEYCEklSLQDIIRDKi---PVDEMWRLF 753  Schizosaccharomy...
Q9LX30     517 ---------------------------ysgavsteipeqdnnlestyLYIQMEYCPr-TLRQVFESYnh-fDKDFAWHLI 567  thale cress
CAG10683   785 asflpsdsdsrsdiifgngdesaddkrqtkdtaetmdsecspliahyLYIQMEYCEksTLRDTIDHGly-qDQNRLWRLF 863  Tetraodon nigrov...
AAC13490   647 yipnmedsdsdgiefvdsngkvavyddeeqedstrgktnspkplmqvMYIQMEFCEkcTLRTAIDDNlf-nDTDRLWRLF 725  fruit fly
EAL17471   670 rgrsqpqsslfqqrpqaqsqrqsipgkpsastsvmtsstsdgmvrriLYIQMEFVEkqTLREAITAGl---TDDEVWRLL 746  Cryptococcus neo...
XP_643344  726 qkklknsnsksksksksksksksksnskksisssklknikkstsipyLYIQMEYCQk-ILRNLTETGmn-lEDDDIWKLF 803  Dictyostelium di...
EAS06885   663 -------nssyydlsslrnsqdssvreqsgknqenqkypvtnfklmiLFIQLEYCPs-TLSHQIKELkfqqDTVQIWQYF 734  Tetrahymena ther...
BAE61949   704 dseeendgtlssrdngqtpetygtgsetgkelsrvrsgsqgrpvsttLYIQMEYCEkhTLRDLIKNGly-dDVDRTWRLF 782  Aspergillus oryzae
XP_421203  757 pttdseseivfdnedenskghspdeegneknsheeedrtpviqtvhyLYIQMEYCEksTLRDTIDRGlh-eDTSRLWRLF 835  chicken
Feature 1                        # #  # #          #                                           
1ZYD_B     123 RQILEALSYIHSQGIIHRDLKPMNIFIDesRNVKIGDFGLAKNVHr------------------SLDILKLDSqnl---- 180  baker's yeast
1ZY4_A     123 RQILEALSYIHSQGIIHRDLKPMNIFIDesRNVKIGDFGLAKNVHr------------------SLDILKLDSqnl---- 180  baker's yeast
Q9HGN1     754 RQILEALAYIHSRGMMHRDLKPGNIFLDenRNVKLGDFGLATENEn------------------YQDNNDKWKnrq---- 811  Schizosaccharomy...
Q9LX30     568 RQIVEGLAHIHGQGIIHRDFTPNNIFFDarNDIKIGDFGLAKFLKle---------------qlDQDGGFSTDva----- 627  thale cress
CAG10683   864 REILDGLAYIHQQGMIHRDLKPVNIFLDsqDHVKIGDFGLATDHPanvaagk-----feveehsTGLMSKPDP------- 931  Tetraodon nigrov...
AAC13490   726 REIAEGLAHIHQQGIIHRDLKPVNIFLDshDQIKIGDFGLATTSFlalqa----------hdaaPAPVNQITSa------ 789  fruit fly
EAL17471   747 RQVLSALAHMASLGIVHRDLKPSNILLDgdGNVKIADFGLSTSEMtai-------------etaSGPIASTID------- 806  Cryptococcus neo...
XP_643344  804 RQIVEGMAYVHGQGIIHRDLKPSNIFFDscGDIKIGDFGLAINNKttst------------lspTTNINSSTSsagsltt 871  Dictyostelium di...
EAS06885   735 RQILEGLNYIHSQNTIHRDLKPENIFIDclGDIKIGDFGLAKSQKqnvfskyqyviekeenelfNQLVNKQYLtek---- 810  Tetrahymena ther...
BAE61949   783 RQILDGLSHIHGHGIIHRDLKPDNIFIDvaNNPRIGDFGLATSGQf------------------TTAVRSSTAad----- 839  Aspergillus oryzae
XP_421203  836 REILDGLAYIHEKGMIHRDLKPVNIFLDsnDHVKIGDFGLATDHPana-------------avsKQEENHSDSfan---- 898  chicken
Feature 1                                                                                      
1ZYD_B     181 ------------------------------------------------pgsSDNLTSAIGTAMYVATEVLdg---tgHYN 209  baker's yeast
1ZY4_A     181 ------------------------------------------------pgsSDNLTSAIGTAMYVATEVLdg---tgHYN 209  baker's yeast
Q9HGN1     812 -------------------------------------------------saDEDLTTGVGTALYVAPELLsrr-ngvRYD 841  Schizosaccharomy...
Q9LX30     628 -------------------------------------------------gsGVDSTGQAGTYFYTAPEIEqd---wpKID 655  thale cress
CAG10683   932 ---------------------------------------------------TDNMTGMVGTALYVSPEVQgnt--kaTYN 958  Tetraodon nigrov...
AAC13490   790 --------------------------------------------------eDGTGTGKVGTTLYVAPELTgna-sksVYN 818  fruit fly
EAL17471   807 ---------------------------------------------------NIDQTSNIGTSLYIAPEVAis----rSYN 831  Cryptococcus neo...
XP_643344  872 ssnsvqsvintkqqqqqqqpqnywedevegqqqqqqqqqqqqqqqqqqqqqQQQHTARVGTLFYTSPEQEagtngdsAYD 951  Dictyostelium di...
EAS06885   811 ------------------------------------------------eleAIDQTLNVGTYFYRPPASQs------MHE 836  Tetrahymena ther...
BAE61949   840 --------------------------------------------------fEGDFTRSLGTTYYVAPEMKsgf--tgNYN 867  Aspergillus oryzae
XP_421203  899 ------------------------------------------------ydpSGNLTGMVGTALYVSPEVQgnt--ksTYN 928  chicken
Feature 1                                                                                    
1ZYD_B     210 EKIDMYSLGIIFFEMIYPF-STGMERVNILKKLRsvsIEFPPDFddn-kmKVEKKIIRLLIDHdPNKRPGaRTLLNSG 285  baker's yeast
1ZY4_A     210 EKIDMYSLGIIFFEMIYPF-STGMERVNILKKLRsvsIEFPPDFddn-kmKVEKKIIRLLIDHdPNKRPGaRTLLNSG 285  baker's yeast
Q9HGN1     842 AKVDMYSLGIILFEMCMTF-STSMERIRIIDTIRspsISFPSTFpfs-raSHEFKVIHCLLQHdPTKRPSsQELLESE 917  Schizosaccharomyce...
Q9LX30     656 EKADMYSLGVVFFELWHPF-GTAMERHVILTNLKl-kGELPLKWvn--efPEQASLLRRLMSPsPSDRPSaTELLKHA 729  thale cress
CAG10683   959 QKVDLFSLGIILFEMSYRPmTTGAERISVLSQLRgvpIVFPQDFctn-ekSTQWRVIEWLLNHdPALRPTtQEVLKSQ 1035 Tetraodon nigrovir...
AAC13490   819 QKVDMYTLGIILFEMCQPPfDTSMERAQTIMALRnvsINIPDAMlkdpkyEKTVKMLQWLLNHdPAQRPTaEELLISD 896  fruit fly
EAL17471   832 EKADMYSLGIIFFEMCYPF-KTTMERVHILTAVRq--PTHDPAAr-----FKATELLRSPLLPtPEKRKEdYDAVIFE 901  Cryptococcus neofo...
XP_643344  952 DKVDMYSLGIVFFEMWYVF-STGHERVIVLRNLRe-kFEFPSDFer--nhSRQATLIRMLIDKdPAKRPSaQQLLQSE 1025 Dictyostelium disc...
EAS06885   837 ERADIYALGIIFFEMWFYF-QSKYQRFKVLTILRn-nNLFPDQFeq--fhPKQAKLIAWMLNFsSQKCQKvMEIMQSD 910  Tetrahymena thermo...
BAE61949   868 DKVDMYSLGVIFFEMCYPL-GTGMERDQTLRAIRekeHTLPPIFrys-ekALQGKIIESLLSHnPSERPSaSELLHSG 943  Aspergillus oryzae
XP_421203  929 QKVDLFSLGIIFFEMSYHPmSTASERIFVLGQLRlpsILFPQDFdev-khAKQRLVITWLLNHdPAARPTaVELLKSE 1005 chicken

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