atypical dual specificity phosphatases similar to yeast Siw14
This subfamily contains Saccharomyces Siw14 and a novel phosphatase from the Arabidopsis thaliana gene locus At1g05000. Siw14, also known as Oca3, plays a role in actin filament organization and endocytosis. Siw14 has been shown to be an inositol pyrophosphate phosphatase, hydrolyzing the beta-phosphate from 5-diphosphoinositol pentakisphosphate (5PP-IP5or IP7). The At1g05000 protein, also called AtPFA-DSP1, has been shown to have highest activity toward olyphosphate (poly-P(12-13)) and deoxyribo- and ribonucleoside triphosphates, and less activity toward phosphoenolpyruvate, phosphotyrosine, phosphotyrosine-containing peptides, and phosphatidylinositols. This subfamily belongs to a group of atypical DSPs present in plants, fungi, kinetoplastids, and slime molds called plant and fungi atypical dual-specificity phosphatases (PFA-DSPs).
Feature 1: catalytic site [active site], 2 residue positions
Conserved feature residue pattern:C R
Evidence:
Comment:the catalytic cysteine initiates a nucleophilic attack on the phosphate group of the substrate, forming a transient phosphoenzyme intermediate and releasing the substrate dephosphorylated; the transition state is stabilized by the arginine present in the catalytic pocket
Jiyao W et al.(2023). "The conserved domain database in 2023.",