4AOD,5BRX,4DBM,2WN9,3WTO,4AOE,2BJ0,4ALX


Conserved Protein Domain Family
LGIC_AChBP

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cd18995: LGIC_AChBP 
Click on image for an interactive view with Cn3D
acetylcholine binding protein (AChBP)
This family contains acetylcholine binding protein (AChBP) which is a soluble extracellular domain homolog secreted by protostomia, and has been widely recognized as a surrogate for the ligand binding domain of nicotinic acetylcholine receptors (nAChRs). AChBP forms a pentameric structure where the interfaces between the subunits provide an acetylcholine (ACh) binding pocket homologous to the binding pocket of nAChRs. Thus far, AChBPs have been characterized only in aquatic mollusks, which have shown low sensitivity to neonicotinoids, the insecticides targeting insect nAChRs. Lymnaea stagnalis acetylcholine binding protein (Ls-AChBP) which has been found in glial cells as a water-soluble protein modulating synaptic ACh concentration has its the binding pocket show better resemblance as it contains all the five aromatic residues fully conserved in nAChR. Five AChBP subunits have been characterized in Pardosa pseudoannulata, a predator enemy against rice insect pests, and share higher sequence similarities with nAChR subunits of both insects and mammals compared with mollusk AChBP subunits.
Statistics
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PSSM-Id: 349796
Aligned: 18 rows
Threshold Bit Score: 207.985
Created: 18-Oct-2017
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 28 residues -Click on image for an interactive view with Cn3D
Feature 1:pentamer interface [polypeptide binding site]
Evidence:
  • Structure:3WTO: Lymnaea stagnalis acetylcholine binding protein forms a homopentamer; contacts at 4.0A
  • Structure:4AOD: Biomphalaria glabrata acetlycholine binding protein forms a homopentamer; contacts at 4.0A

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                # #   # ###                           # #         # # #    ## #      #
4AOD_A      29 VKVSFEYSLqKIFRADVeNDEVDIgLWTTLVWKDRCLNWFn-eFTSFKELTVPIAEIWTPDIFIFDs-vGAPEif---sD 103  Biomphalaria gla...
3WTO_A      31 VAVSVSLKFiNILEVNEiTNEVDVvFWQRTTWSDRTLAWDs--SHSPDQVSVPISSLWVPDLAAYNa-iSKPEvl---tP 104  great pond snail
2BJ0_A      25 LNVSLNFKLmNIVEADTeKDQVEVvLWTQASWKVPYYSSLl-sSSSLDQVSLPVSKMWTPDLSFYNa-iAAPEll---sA 99   Bulinus truncatus
ABI96921    48 VNVTVSLTLiDILKVDKeTGQVDIvAWVSNTWKSNFQWDPa-rFGNVSNLAIPSKLLWVPDIYAYNg-iSKMEpl---sP 122  Pleurobranchaea ...
ABI96922    51 VKLGLSMTLiDIKDVDQrKNQVEIdAWLAMTWNCGITFDPt-eFNNLMNVRLPPSKVWVPDLAVYNgdiDLLGyi---gH 126  Tritonia diomedea
EEN48941    33 VVVDVSMTVaSILAVDDkNQELKTlSWLQMSWKDYRLRWNpgqYDNIRKINIPSDQLWRPDLLPYNs-rTLDSgl--dlT 109  Florida lancelet
ABI96923    51 ISVKIGFQMrYISSLDEeTNTLNVeGWLLMKWKDADLQWDpeeFGGVKELYIDSSDIWTPDIALYEs-sSADNffp-raQ 128  Atlantic horsesh...
ARU12131    54 VPVKISISPlSLRSMNEkNQIISLeAWMMLTWMDEYLRWDpsdYDNITELRISPTEIWKPDIALYTa-sPDTSlfp-vvH 131  Pardosa pseudoan...
ARU12132    53 VPVSVQITPlALRSVDFdSNILKLdTWLTVQWHDEYLHWDpteFGGQENLILPASEVWKPDIAVYTa-tHDSAslp-hvE 130  Pardosa pseudoan...
KZS07084   690 IKLEAGLTL-TNFNFDElESKFSAkGFFYMQWKDPRFMWSpadYEGLKSIHLPSEKVWKPDLEVYNs-yGSLSndrsfaS 767  Daphnia magna
Feature 1       # #         #   #   # #                      ####  #                       #   
4AOD_A     104 KLARVSQDGTVTYVPQLKVRLSCPLADl----kLETGVTCSLKSGSWTHSTqELTLEVNAKVdlgdy-----asdtRFQL 174  Biomphalaria gla...
3WTO_A     105 QLARVVSDGEVLYMPSIRQRFSCDVSGv----dTESGATCRIKIGSWTHHSrEISVDPTTENsddsey---fsqysRFEI 177  great pond snail
2BJ0_A     100 DRVVVSKDGSVIYVPSQRVRFTCDLINv----dTEPGATCRIKVGSWTHDNkQFALITGEEGvvniae---yfdspKFDL 172  Bulinus truncatus
ABI96921   123 DLLVIAHTGDVVYVPSIRISFLCDLKRq----dISTNATCELKLGSWVYSGyKMDLINPRFDlssy------fsgsKYEV 192  Pleurobranchaea ...
ABI96922   127 QNVLLQYDGNALWIPPSKIRASCDLADd----kLLTNATCRVKIGSWTYSAhHIYLNDETSVdissy-----nqdsKYNV 197  Tritonia diomedea
EEN48941   110 VDAIVSSDGEVTFIPKWLTQTSCAFDAty---yPFDEQTCDLKVGSWTHHNrEIDLRLGLESislep----fvedsTWQI 182  Florida lancelet
ABI96923   129 VKLIARNTGEVLWVPPVSFQTICPLVP------NSYEQRCNLTLGSWVRSIrDIDVHPDPDTdpdges--fvrvhqKWEL 200  Atlantic horsesh...
ARU12131   132 TEAIVYNNGMIIWVPPFTINSRCPIPFrsyvsvSRTFVDCNIRMGSWTYSGkMVDLQLSTDKvdltnf---qdynyEWKL 208  Pardosa pseudoan...
ARU12132   131 TNVLIHSNGDVLWVPPFTIENKCPTLNs-----HEIRQECDISMLSWTYDItQLDLNIGNATsleyf----iddepRWHL 201  Pardosa pseudoan...
KZS07084   768 NDVLITSDGSVYFVPISTLDSICIPDAtl---yPFDSVTCTTSIGSWTHNAlEIDMNTRNNAttvdvkdlisrrmsQWKL 844  Daphnia magna
Feature 1                   #                 
4AOD_A     175 LNATQQVNRKQYpCCPETYEDTTLSFTFRKP 205  Biomphalaria glabrata
3WTO_A     178 LDVTQKKNSVTYsCCPEAYEDVEVSLNFRKK 208  great pond snail
2BJ0_A     173 LSATQSLNRKKYsCCENMYDDIEITFAFRKK 203  Bulinus truncatus
ABI96921   193 LGVSAKKETSYYqCCPEPYISLKFVLSFKEQ 223  Pleurobranchaea californica
ABI96922   198 TNVSAKKVARTYsCCPEEYHHILFTFSFVKK 228  Tritonia diomedea
EEN48941   183 KKTQATRHVMKYeCCPEAYVHILFSVSFSRS 213  Florida lancelet
ABI96923   201 IDVNIIRKDAIYsCCPENYPSIVHTLHFKLR 231  Atlantic horseshoe crab
ARU12131   209 VKIVSNRESKLYpCCADEYPHVNFNVTLKKR 239  Pardosa pseudoannulata
ARU12132   202 NSITPLRENKLFsCCKEFYPTIHFKVVLTDK 232  Pardosa pseudoannulata
KZS07084   845 MSTNVSIEVKVYeCCPEPYASLRLSFTIQRS 875  Daphnia magna

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