1II6,4AP0,3HQD,1Q0B,1X88,4A1Z,4A28,4B7B,4AQV,2WBE


Conserved Protein Domain Family
KISc_BimC_Eg5

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cd01364: KISc_BimC_Eg5 
Click on image for an interactive view with Cn3D
Kinesin motor domain, BimC/Eg5 spindle pole proteins
Kinesin motor domain, BimC/Eg5 spindle pole proteins, participate in spindle assembly and chromosome segregation during cell division. This catalytic (head) domain has ATPase activity and belongs to the larger group of P-loop NTPases. Kinesins are microtubule-dependent molecular motors that play important roles in intracellular transport and in cell division. In most kinesins, the motor domain is found at the N-terminus (N-type), N-type kinesins are (+) end-directed motors, i.e. they transport cargo towards the (+) end of the microtubule. Kinesin motor domains hydrolyze ATP at a rate of about 80 per second, and move along the microtubule at a speed of about 6400 Angstroms per second. To achieve that, kinesin head groups work in pairs. Upon replacing ADP with ATP, a kinesin motor domain increases its affinity for microtubule binding and locks in place. Also, the neck linker binds to the motor domain, which repositions the other head domain through the coiled-coil domain close to a second tubulin dimer, about 80 Angstroms along the microtubule. Meanwhile, ATP hydrolysis takes place, and when the second head domain binds to the microtubule, the first domain again replaces ADP with ATP, triggering a conformational change that pulls the first domain forward.
Statistics
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PSSM-Id: 276815
Aligned: 22 rows
Threshold Bit Score: 502.626
Created: 5-Feb-2015
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
ATP bindingmicrotubule
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Structure:1II6: Human Mitotic Kinesin Eg5 bound to Mg-ADP
  • Comment:interaction site for Mg ADP, ATP hydrolysis drives motor function

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                #                                                                     
1II6_A      16 GKNIQVVVRCRPFNlae-rkaSAHSIVECDPv-rKEVSVRtggla----------------------------------- 58   human
EAK86201   168 ESNIQVVVRVRGQApne-pkrTAPGILTTSGprcQQIDVAieapqvssssaia-------------------stsnlvqe 227  Ustilago maydis 521
AAR39442    21 EQNIQAFVRVRPFSvyelnqgVTTVPIRVSDs-sKEVLCEyk-------------------------------------- 61   Dictyostelium di...
AAK91815    50 SVNVQVLLRCRPFSdde-lrnNAPQVITCNDy-qREVAVTqnia------------------------------------ 91   maize
CAD98462    15 GVNVKVIVRCRPLTeqekkdpSNSNVLQVKPd-sKEIVVShqslsr--------------------------------kf 61   Cryptosporidium ...
AAG18583    11 EKNIQVVVRCRPVNsie-kkqNSYSVLDVKPs-kKEICVSteva------------------------------------ 52   purple urchin
P46863      17 NQNIQVYVRVRPLNsre-rciRSAEVVDVVGp--REVVTRhtld------------------------------------ 57   fruit fly
P28742      50 DSNIHVYVRCRSRNkre-ieeKSSVVISTLGpqgKEIILSngshq----------------------------------s 94   Saccharomyces ce...
EAK93953     1 MSNIQVVVRCRGRNsqe-iaaKSPIVVELTNd-nYSITEPyitinqgytratattttnnttttnsnntagatpntsassl 78   Candida albicans...
P24339      70 ETNINVVVRVRGRTdqe-vrdNSSLAVSTSGamgAELAIQsdps------------------------------------ 112  Schizosaccharomy...
Feature 1                                                    #  # ####                         
1II6_A      59 dkssRKTYTFDMVFGASTKQIDVYRSVVCPILDEVIMGYNCTIFAYGQTGTGKTFTMEGErspneey----tweedplAG 134  human
EAK86201   228 satrQKSYHFDQVFGPEADQGMVYQDVVGPILEEVMSGYNCTIFAYGQTGTGKTHTMEGDltsqmg-------tysseAG 300  Ustilago maydis 521
AAR39442    62 --gtTRQYKFDHVFDQDSIQSEVFNIAVKPICDEVLLGFNGTIFVYGQTGTGKTHTMEGKhgs------------nedMG 127  Dictyostelium di...
AAK91815    92 gkqfDRVYAFDKVFGPTAKQKELYDQAIIPIVNEVLEGFNCTIFAYGQTGTGKTYTMEGEcrraksgp---kgqlpadAG 168  maize
CAD98462    62 dsysTKLFTFDGVCGSFTSQRELFKQYVVPIVDEVLLGFNCTIFAYGQTGTGKTYTMEGDmkeyless---nleltehAG 138  Cryptosporidium ...
AAG18583    53 ekasSKIFSFDKVFGPKSPQIEVYKSVVAPILDEVLMGYNCTVFAYGQTGTGKTFTMEGErtpdpdl----sweqdplAG 128  purple urchin
P46863      58 -sklTKKFTFDRSFGPESKQCDVYSVVVSPLIEEVLNGYNCTVFAYGQTGTGKTHTMVGNetaelks----sweddsdIG 132  fruit fly
P28742      95 ysssKKTYQFDQVFGAESDQETVFNATAKNYIKEMLHGYNCTIFAYGQTGTGKTYTMSGDinilgdvqstdnlllgehAG 174  Saccharomyces ce...
EAK93953    79 dtnsKRTYKFDQIYGSQADQNLIYSQIAHPLLLDFLRGINVTVLAYGQTGTGKTYTMSGMdnnnkl------densdiAG 152  Candida albicans...
P24339     113 smlvTKTYAFDKVFGPEADQLMLFENSVAPMLEQVLNGYNCTIFAYGQTGTGKTYTMSGDlsdsdg-------ilsegAG 185  Schizosaccharomy...
Feature 1                                                                                      
1II6_A     135 IIPRTLHQIFEKLTdn---gTEFSVKVSLLEIYNEELFDLLNpss---------------dvsERLQMFDdp--rnKRGV 194  human
EAK86201   301 IIPRSLYRLFHTLEls---kEDYSVKATFIELYNEELRDLLSidsstssaepsssatatkepqHALRMYDda---rGKGV 374  Ustilago maydis 521
AAR39442   128 IIPRTINYLFQCLEqs---gADYNIRASHLEIYKEEIFDLLAcngn-------------enlnKPLGMFDt-----QKGF 186  Dictyostelium di...
AAK91815   169 VIPRAVKQIFDTLErq---nTEYSVKITFLELYNEEITDLLApeeiskaa-----fedkqkkaLPLNLMEd----gKGGV 236  maize
CAD98462   139 IIPRAVQLIFERLEsq---yTEYGVRVSYLEIYNEELSDLLSde------------------kLNLRIYDdi--agKRGL 195  Cryptosporidium ...
AAG18583   129 IIPRAMHQIFEKMIgt---dIEFSVRVSYLELYNEELFDLLSsqe----------------dtQRLRIFEds--arKGSV 187  purple urchin
P46863     133 IIPRALSHLFDELRmm---eVEYTMRISYLELYNEELCDLLStdd----------------ttKIRIFDDst---kKGSV 190  fruit fly
P28742     175 IIPRVLVDLFKELSsl---nKEYSVKISFLELYNENLKDLLSdsedddp--------avndpkRQIRIFDnn--nnNSSI 241  Saccharomyces ce...
EAK93953   153 IIPRLLGELFQNLSsnatskNDYMVKISYLEIYNEELIDLLSnnn---------------snnRKLRIHEktnankVKSI 217  Candida albicans...
P24339     186 LIPRALYQLFSSLDns---nQEYAVKCSYYELYNEEIRDLLVsee----------------lrKPARVFEdt--srRGNV 244  Schizosaccharomy...
Feature 1                                                                                      
1II6_A     195 IIKGLEEITVHNKDEVYQILEKGAAKRTTAATLMNAYSSRSHSVFSVTIHMKETtid------------geELVKIGKLN 262  human
EAK86201   375 VIQGLEEVALKDAAHGLSVLRRGSQKRQIAATNCNEQSSRSHSVFTMTVFIKDKgsr------------geDVLKIGKLN 442  Ustilago maydis 521
AAR39442   187 KIPELEEIVVNDRQSILNVLAKSCKRRQTAETQYNKQSSRSHCIFSITVHVKETsvg------------geDLIKIGKLN 254  Dictyostelium di...
AAK91815   237 LVRGLEEEIVTNASEIFSLLERGSAKRRTAETLLNKQSSRSHSLFSITIHIKEAtpe------------geELIKCGKLN 304  maize
CAD98462   196 NVDRLEEIPVNKAQDILNILSTAVRKRRTAETLLNKSSSRSHCIFTITIHTKETnid------------geDVLKVGKLN 263  Cryptosporidium ...
AAG18583   188 VIQGLEEVTVHNKNEVYAILEKGAAKRKTAATLMNAHSSRSHSVFSVTIHIKENsie------------gdELLKTGKLN 255  purple urchin
P46863     191 IIQGLEEIPVHSKDDVYKLLEKGKERRKTATTLMNAQSSRSHTVFSIVVHIRENgie------------geDMLKIGKLN 258  fruit fly
P28742     242 MVKGMQEIFINSAHEGLNLLMQGSLKRKVAATKCNDLSSRSHTVFTITTNIVEQdskdh--------gqnkNFVKIGKLN 313  Saccharomyces ce...
EAK93953   218 GIQNLTEYTINNYNQGIKYLKMGLQKKKTSSTNLNETSSRSHTIFSVQLYQKISsssssss----endneeSVYRISKMN 293  Candida albicans...
P24339     245 VITGIEESYIKNAGDGLRLLREGSHRRQVAATKCNDLSSRSHSIFTITLHRKVSsgmtdetnsltinnnsdDLLRASKLH 324  Schizosaccharomy...
Feature 1        #                                                                             
1II6_A     263 LVDLAGSENIGRSGAvd--kRAREAGNINQSLLTLGRVITALVERT-PHVPYRESKLTRILQDSLGGRTRTSIIATISPA 339  human
EAK86201   443 LVDLAGSENIGRSGAen--kRAREAGMINQSLLTLGRVINALVEKN-SHIPYRESKLTRLLQESLGGRTKTCIIATVSQE 519  Ustilago maydis 521
AAR39442   255 LVDLAGSENAEKSGNn---dRLREAALINKSLLTLGKVITDLTNNE-KHIPYRSSQLTKILQDSLGGKTKTSIIATVSPS 330  Dictyostelium di...
AAK91815   305 LVDLAGSENISRSGAke--gRAREAGEINKSLLTLGRVITALVEHL-GHVPYRDSKLTRLLRDSLGGRTKTCIIATVSPS 381  maize
CAD98462   264 LVDLAGSENIQRSGAnavkdRAKEAGMINQSLLTLGRVINALVEHS-SYVPYRDSKLTRLLQDSLGGRTKTCIIATITAS 342  Cryptosporidium ...
AAG18583   256 LVDLAGSENIGRSGAvd--kRAREAGNINQSLLTLGRVITALVEHA-PHVPYRESKLTRLLQDSLGGRTKTSIIATVSPA 332  purple urchin
P46863     259 LVDLAGSENVSKAGNek-giRVRETVNINQSLLTLGRVITALVDRA-PHVPYRESKLTRLLQESLGGRTKTSIIATISPG 336  fruit fly
P28742     314 LVDLAGSENINRSGAen--kRAQEAGLINKSLLTLGRVINALVDHS-NHIPYRESKLTRLLQDSLGGMTKTCIIATISPA 390  Saccharomyces ce...
EAK93953   294 LVDLAGSENISRSGS-----IVKEAGGINQSLLTLGRVINSLNEKKlSHIPYRESKLTHILQDSLGGNTKTTLIATISPA 368  Candida albicans...
P24339     325 MVDLAGSENIGRSGAen--kRARETGMINQSLLTLGRVINALVEKA-HHIPYRESKLTRLLQDSLGGKTKTSMIVTVSST 401  Schizosaccharomy...
Feature 1                                   
1II6_A     340 SLNLEETLSTLEYAHRAKNILNKPEVNQK 368  human
EAK86201   520 RANIEETLSTLDYALRAKSIKNRPELNTR 548  Ustilago maydis 521
AAR39442   331 LVNLEETINTLEYALKAKNIKNTPQINQR 359  Dictyostelium discoideum
AAK91815   382 VHCLEETLSTLDYAHRAKSIKNRPEVNQK 410  maize
CAD98462   343 SIYLEETLNTLDYAHRAKNIKNMPVVNQK 371  Cryptosporidium parvum
AAG18583   333 SINVEETLSTLDYAHRAKHITNRPEINQK 361  purple urchin
P46863     337 HKDIEETLSTLEYAHRAKNIQNKPEVNQK 365  fruit fly
P28742     391 KISMEETASTLEYATRAKSIKNTPQVNQS 419  Saccharomyces cerevisiae S288c
EAK93953   369 QVNLSETCSTLDYASKAKNIKNAPMIGHD 397  Candida albicans SC5314
P24339     402 NTNLEETISTLEYAARAKSIRNKPQNNQL 430  Schizosaccharomyces pombe 972h-

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