3DW9


Conserved Protein Domain Family
SgrAI-like
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cd22360: SgrAI-like 
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Restriction endonuclease SgrAI and similar nucleases
The type II restriction endonuclease SgrAI binds and cleaves the target sequence CR|CCGGYG (| denotes the cleavage site, R stands for a purine and Y stands for a pyrimidine). It belongs to a superfamily of nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.
Links
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Statistics
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PSSM-Id: 411764
Aligned: 9 rows
Threshold Bit Score: 426.037
Created: 28-Mar-2011
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:3DW9: Streptomyces griseus restriction endonuclease SgrAI active site binds substrate DNA and two divalent cations; contacts at 4A
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                        #                               
3DW9_B        62 DYRDVIGRPVENAVTEGTHFFr--------dDFRVDSNAKAKVAGDIFEIVSSAVMWNCAARWNSLMVGEGWRSQPRYSR 133 Streptomyces gr...
ADZ31424      63 SFEEYLKKFTSNAMLAGETLFg--------gEFNVKGAAIAKVEGDVFELLEAAALWNATAAWNRLMDSGSWGASVFTCP 134 Pseudomonas stu...
WP_077962956  21 LFADYRRQAYENVDTAGHVIWp--------gAFELTEAQKAKVDGDIYETMEAAALWNAAAVWNSFMDTGVWSSAVFTQP 92  Ensifer adhaerens
AVT07712      21 TYDTYLNQFRSNALTAGSALFg--------tAFDVKSSAIAKVEGDVFELLEAGALWAAASAWNNFMNTGNWISEAFTCP 92  Acidovorax aven...
OJY44287      21 TFGEYLGQFRSNAHGALSVLYg--------aGFAFSGSALAKVEGDVFELMEAGAIWNAFAAWNKFMDGLPWPSKVFTTP 92  Rhizobiales bac...
WP_072296898  44 PFAIYRAQAIANLHAAGAINFpdvgdelgyrAGSINAQALAKVQGDIYEILEAAALWNAAADWNAFMDTGFWPAGPFEQP 123 Paracoccus sp. ...
WP_108742848  63 PFREVALQPVNNANDEGRLLFg--------vDYNVNANAVAKVTGDIYETVTSAVLWNAVAAWNRVMRGDAWQQSPRYAR 134 Clavibacter mic...
PRZ39649      63 PFQEVVRQPIANARAEGDYLYg--------sDFTLDSNAIAKVTGDIYETLTSAILWDAAAYWNSFMATGNWPTPRRYAR 134 Antricoccus suf...
WP_064919233  27 DIRELVQQGINNADEEGELRFq--------qDYVVSGNAKAKVLGDLYETITTAMLWNAAAMWNMYMTTGDWPATPRYTK 98  Mycolicibacteri...

Feature 1                      #                                      #                          
3DW9_B       134 PTLSPSPRRQVAVLNLPRSFDWVSLLVPESQEVIEEFRAGLRKDGLGLPTSTPDLAVVVLPEEfq-ndEMWREEIAGLTR 212 Streptomyces gr...
ADZ31424     135 QSAVPTPTRKIAVVTLPRGYDATKLFRDEIRSSIRAHEEALHLRGLSLGLSSPDIVGVRLPCPlpeelGCFMEPIENLGE 214 Pseudomonas stu...
WP_077962956  93 KNAVPSPARKVAIVKMARGADTTLILNDKARAEYKAFEVALQTRGMELKLSTPDILGLRMPDPmpegfRPFLSPLPDLTH 172 Ensifer adhaerens
AVT07712      93 PGAVATPTRKIAIVKLPRGYDATRLFKPEIRATIKAHESALRLRGMELGLSSPDIVGVRLPYPlppslSMFMSPVPDLKQ 172 Acidovorax aven...
OJY44287      93 NGTVATPSRKAAILKLPRGYDTTRLFKSEVRTRIQAHEQALKLRGMELGLSSPDIVGIRIPDPmppefAPFLDPLPNLGE 172 Rhizobiales bac...
WP_072296898 124 AGSVPTPLRRIAIVKLPRNYDSTRLLTNASRAAYQAFQQSLERQGMVLRLSSPDIVGVRIPDPmpdayQSFLRRLPDLSM 203 Paracoccus sp. ...
WP_108742848 135 PRIAGSLNRQIAVLNLPRRYDWVRLLTPRAQADIATMRGGLRAAGLGMPTSTPDVAIIVLPQSeq-gnPVWATSLDDLSL 213 Clavibacter mic...
PRZ39649     135 PSVGRSERRQVGIVNLPRRYDWVRLLEPNAKARIAVLRDKLAENNLSMPTSTPDIAVVVLPDEvr-kdDIWRTPFLNLDR 213 Antricoccus suf...
WP_064919233  99 PTLSPLPSRQVAVLNLPRGYDWVRLLNAEARQAIEVVRAPLRAKDLTIPTSTPDIAIVVLPDAasrldDRWRTPLDNLKL 178 Mycolicibacteri...

Feature 1                                  # #             #                                     
3DW9_B       213 PNQILLSGAYQRLQGRVQPGEISLAVAFKRSLRSDRLYQPLYEANVMQLLLEGKLGAPKVEFEVHTLAPEGTNAFVtyEA 292 Streptomyces gr...
ADZ31424     215 ENRVKLEEAYKLLEGKIEATGFLFAIAVKRTIRSDRLYQPLFEANVLKYLIEVVLKGAAFRFYAHFNSFEGADVEGhyKA 294 Pseudomonas stu...
WP_077962956 173 GSQEILDTAWQGIQGTLEGRHFLFAIAVKTSTRSDRLYQALFEANVLKYILGYVLRGPAIKFHAHLETFANADVVGryKA 252 Ensifer adhaerens
AVT07712     173 PNLDLLEGAHRLLEDTIDATGFLLAIAVKRTTRSDRLYQPLFESNILKYLIEFVLRGAAFRFYAHLGSFEGADVVGryRA 252 Acidovorax aven...
OJY44287     173 QARLILEKTHEKLEGTLEGRSFLFAIAVKRTTRSDRLYQPLFEANVLKYLIEEVLRGAAFRFHVHMGSFEGADVEGhyNA 252 Rhizobiales bac...
WP_072296898 204 ANYRLLQGAHQAIEGTLDGRNFLFAIAVKTSTRSDRLYQPLFEANVLKFIIGFVLRGSSLRFHAHMENFDGADVEGayRA 283 Paracoccus sp. ...
WP_108742848 214 ANQRVLDHAHTLVEHKVEPGEIILAIALKTSLRSDRLYQPLYEANVMEFLLEEHLGAPRVEFEVHTLDSSGTRAAEiyQA 293 Clavibacter mic...
PRZ39649     214 GSQGTLSTAHTRLADSVEPGEIILAMALKSSLRSDRLYQPLYEANVMQLLLERHLGAPRVEFEVHALTAEGTGATAiyTA 293 Antricoccus suf...
WP_064919233 179 PQQTILNSSYGELAGRIEPGELILAIAAKRSLRSDRLYQPLYEANIMQLLLEHFLRAPRVDFEVHTLSIEGTAAMDi-YA 257 Mycolicibacteri...

Feature 1                                                      
3DW9_B       293 ASLYGLAEgr-saVHRAIRELYVPPTa--aDLARRFFAFLNERMEL 335 Streptomyces griseus
ADZ31424     295 ASLISLARgg--tPTKAIDVLHLAES----PLASAQAVLNDFPLFH 334 Pseudomonas stutzeri
WP_077962956 253 ASMTSLLAgg--tPAKAIDETYLALS----PRDTAQHILDQLPEFP 292 Ensifer adhaerens
AVT07712     253 ASLISLIRgg--tPGPAIDSLYHAER----PRDSAQSVMNDFPLFP 292 Acidovorax avenae subsp. avenae
OJY44287     253 ASLVSLMRgg--ePTKAVTSTYLAER----PVECAQTILNDLPLFP 292 Rhizobiales bacterium 64-17
WP_072296898 284 ASLPSLLLgg--rPQKAIDKLYQAVN----PRATAQEILNEMPLFP 323 Paracoccus sp. SM22M-07
WP_108742848 294 AALSSAAGp---aPHRAIRELYTPANat-eVVQRFLAFLNDRMALV 335 Clavibacter michiganensis
PRZ39649     294 ASLHSAGE-----PTAHRAVRELYEPanatQIVQRFFAFLNQRMAT 334 Antricoccus suffuscus
WP_064919233 258 AATIYAVAvnspkVHRSVRELYIPTTp--mPLVDRFLAFLDQRMAS 301 Mycolicibacterium elephantis

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