GEO DataSets

(Submitter supplied) A prototype oligonucleotide microarray was designed to detect and identify viable bacterial species with the potential to grow of common beer spoilage microorganisms from the genera Lactobacillus, Megasphaera, Pediococcus and Pectinatus. Probes targeted the intergenic spacer regions (ISR) between 16S and 23S rRNA, which were amplified in a combination of reverse transcriptase (RT) and polymerase chain reaction (PCR) prior to hybridization. more...

Organism:

Lacticaseibacillus casei; Megasphaera cerevisiae; Pectinatus cerevisiiphilus; Loigolactobacillus coryniformis; Schleiferilactobacillus perolens; Pediococcus inopinatus; Pectinatus frisingensis; Levilactobacillus brevis; Pediococcus damnosus

Type:

Expression profiling by array

Platforms:

GPL5157 GPL5158

12 Samples

Download data: GPR

(Submitter supplied) Each oligonucleotide species-specific probe was spotted in 70 replicate spots on the microarray. Nine single beer spoilage bacteria species were detected with the developed prototype oligonucleotide microarray and also three mixes of two different beer spoilage bacteria species. Protocol: Standard glass microscope slides were coated with a poly-L-lysine solution and stored for several weeks to allow the surface to become sufficiently hydrophobic. more...

Organism:

Pectinatus frisingensis; Levilactobacillus brevis; Lacticaseibacillus casei; Loigolactobacillus coryniformis; Megasphaera cerevisiae; Pediococcus damnosus; Pectinatus cerevisiiphilus; Schleiferilactobacillus perolens; Pediococcus inopinatus

1 Series

8 Samples

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