GEO DataSets

(Submitter supplied) Methylated DNA enrichment is a key step in a microarray based genome-wide methylation profiling study, and even for future high-throughput sequencing based methylome analysis. In order to evaluate the sensitivity and accuracy of methylated DNA enrichment, we investigated and optimized a number of important parameters to improve the performance of several enrichment assays, including differential methylation hybridization (DMH), microarray-based methylation assessment of single samples (MMASS), and methylated DNA immunoprecipitation (MeDIP). more...

Organism:

Homo sapiens

Type:

Methylation profiling by array

Platform:

GPL9952

17 Samples

Download data: LSR

(Submitter supplied) Clone:pGEM-5Zf(-)/Primers:5'-CGG CCG CCT GCA GGT CGA CCT TAA- 3';5'-AAC GCG TTG GGA GCT CTC CCT TAA- 3';oligo synthesize/strandedness:Methylation tolerant E.coli strain XLI-BLUE MRF'/Length:760 bp(Average insert size);Arrayer Harderware:Aminosilane Coated Glass Slides/Software:Arrays were scanned with a confocal LuxScanTM scanner (CapitalBio Corp.), and obtained images were analyzed with LuxScan3.0 TM software (CapitalBio Corp);Spotting protocol:The PCR products were precipitated with ethanol and re-dissolved in EasyArrayTM Spotting Solution (CapitalBio Corp.), then printed in triplicate on Aminosilane Coated Glass Slides using a SmartArrayTM microarrayer (All from CapitalBio Corp.).

Organism:

Homo sapiens

1 Series

17 Samples

Download data: GAL