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Items: 8

1.

AGO4 slicer activity reveals the passenger strand function of plant 23 nt siRNAs and the post-slicing retention of target RNAs mediating RNA-directed DNA methylation [smallRNA-seq]

(Submitter supplied) In plants, transcriptional silencing by RNA-directed DNA methylation (RdDM) is mediated by ARGONAUTE 4 (AGO4) and 24 nt short-interfering RNAs (siRNAs) that are generated in parallel with 23 nt RNAs of unknown function. We show that 23 nt RNAs serve as the passenger strands of 23/24 nt duplexes loaded into AGO4. The 24 nt siRNAs then guide AGO4 slicing of the passenger strands, generating 11 and 12 nt cleavage products. more...
Organism:
Arabidopsis thaliana
Type:
Non-coding RNA profiling by high throughput sequencing
Platform:
GPL19580
6 Samples
Download data: TXT
Series
Accession:
GSE201233
ID:
200201233
2.

Illumina NextSeq 500 (Arabidopsis thaliana)

Organism:
Arabidopsis thaliana
390 Series
8865 Samples
Download data
Platform
Accession:
GPL19580
ID:
100019580
3.

sRNA-seq, slicing-defective AGO4 immunoprecipitation, biol rep 2

Organism:
Arabidopsis thaliana
Source name:
Inflorescence
Platform:
GPL19580
Series:
GSE201233 GSE201235
Download data: TXT
Sample
Accession:
GSM6053233
ID:
306053233
4.

sRNA-seq, wild-type AGO4 immunoprecipitation, biol rep 2

Organism:
Arabidopsis thaliana
Source name:
Inflorescence
Platform:
GPL19580
Series:
GSE201233 GSE201235
Download data: TXT
Sample
Accession:
GSM6053232
ID:
306053232
5.

sRNA-seq, mock immunoprecipitation, biol rep 2

Organism:
Arabidopsis thaliana
Source name:
Inflorescence
Platform:
GPL19580
Series:
GSE201233 GSE201235
Download data: TXT
Sample
Accession:
GSM6053231
ID:
306053231
6.

sRNA-seq, slicing-defective AGO4 immunoprecipitation, biol rep 1

Organism:
Arabidopsis thaliana
Source name:
Inflorescence
Platform:
GPL19580
Series:
GSE201233 GSE201235
Download data: TXT
Sample
Accession:
GSM6053230
ID:
306053230
7.

sRNA-seq, wild-type AGO4 immunoprecipitation, biol rep 1

Organism:
Arabidopsis thaliana
Source name:
Inflorescence
Platform:
GPL19580
Series:
GSE201233 GSE201235
Download data: TXT
Sample
Accession:
GSM6053229
ID:
306053229
8.

sRNA-seq, mock immunoprecipitation, biol rep 1

Organism:
Arabidopsis thaliana
Source name:
Inflorescence
Platform:
GPL19580
Series:
GSE201233 GSE201235
Download data: TXT
Sample
Accession:
GSM6053228
ID:
306053228
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Supplemental Content

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