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Items: 8

1.

Whole genome enzymatic methyl-seq of A20 mouse cells using a novel TETact activation of CD4

(Submitter supplied) Stably silenced genes that display a high level of CpG dinucleotide methylation are refractory to the current generation of CRISPR dCas9-based activation systems. To counter this, we created an improved activation system by coupling the catalytic domain of DNA demethylating enzyme TET1 with transcriptional activators (TETact). To evaluate the specificity of our TETact system, we conducted RNA-seq on A20 cells containing the TETact-v3 system targeting the CD4 promoter, along with non-targeting control TETact-v3 cells as well as wildtype A20. more...
Organism:
Mus musculus
Type:
Methylation profiling by high throughput sequencing
Platform:
GPL30172
6 Samples
Download data: COV
Series
Accession:
GSE211754
ID:
200211754
2.

NextSeq 2000 (Mus musculus)

Platform
Accession:
GPL30172
ID:
100030172
3.

A20 with non-targeting control sgRNA replicate 2

Organism:
Mus musculus
Source name:
A20 B cell lymphoma cell line
Platform:
GPL30172
Series:
GSE211754 GSE212345
Download data: COV
Sample
Accession:
GSM6500675
ID:
306500675
4.

A20 with non-targeting control sgRNA replicate 1

Organism:
Mus musculus
Source name:
A20 B cell lymphoma cell line
Platform:
GPL30172
Series:
GSE211754 GSE212345
Download data: COV
Sample
Accession:
GSM6500674
ID:
306500674
5.

A20 with sgRNA activating CD4 gene replicate 2

Organism:
Mus musculus
Source name:
A20 B cell lymphoma cell line
Platform:
GPL30172
Series:
GSE211754 GSE212345
Download data: COV
Sample
Accession:
GSM6500673
ID:
306500673
6.

A20 with sgRNA activating CD4 gene replicate 1

Organism:
Mus musculus
Source name:
A20 B cell lymphoma cell line
Platform:
GPL30172
Series:
GSE211754 GSE212345
Download data: COV
Sample
Accession:
GSM6500672
ID:
306500672
7.

Wild-type A20 replicate 2

Organism:
Mus musculus
Source name:
A20 B cell lymphoma cell line
Platform:
GPL30172
Series:
GSE211754 GSE212345
Download data: COV
Sample
Accession:
GSM6500671
ID:
306500671
8.

Wild-type A20 replicate 1

Organism:
Mus musculus
Source name:
A20 B cell lymphoma cell line
Platform:
GPL30172
Series:
GSE211754 GSE212345
Download data: COV
Sample
Accession:
GSM6500670
ID:
306500670
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Supplemental Content

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