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Items: 3

1.

A Global Transcriptome Analysis of BMSC from different confluences, But Gene Expression Profiling Suggested Comprised Pro-angiogenesis Effects

(Submitter supplied) Ex vivo expansion of Bone Marrow Stromal Cells (BMSC) is required to obtain clinical dose for cellular therapy, and different labs use different confluence in their practice, however, the impacts of 100% confluence on the biological properties of BMSC remain controversial. In this study, we detected the changes occurred to BMSCs when they reached 100% confluence, including viability, population doubling time (PDT), apoptosis, colony formation, immunosuppression, proteins in the culture supernatant, and surface markers; we also performed gene expression profiling and microRNA profiling on 50%, 80% and 100% confluent BMSCs. more...
Organism:
Mus musculus; Human gammaherpesvirus 8; Homo sapiens; Rattus norvegicus; human gammaherpesvirus 4; Betapolyomavirus macacae
Type:
Non-coding RNA profiling by array
Platform:
GPL19084
15 Samples
Download data: GPR
Series
Accession:
GSE60449
ID:
200060449
2.

CCDTM miRNA-version 5

(Submitter supplied) Oligos sythesized by Operon with 3' amine modification and purified by desalting. Oligos are adjusted to the same concentration and resuspend in 2mM phosphate buffer at 25mM oligo concentration and spotted on CodeLink(TM) Activated Slides (GE Healthcare) using an Omnigrid robotic arrayer (Genomic Solutions).
Organism:
Homo sapiens; Mus musculus; Rattus norvegicus; human gammaherpesvirus 4; Human gammaherpesvirus 8; Betapolyomavirus macacae
1 Series
15 Samples
Download data
Platform
Accession:
GPL19084
ID:
100019084
3.

Donor 2 - 09FC43 - 100% confluence - mAdbID:107353

Organism:
human gammaherpesvirus 4; Homo sapiens
Source name:
EBV-infected lymphcytes (channel 1) Donor 2 - 09FC43 (channel 2)
Platform:
GPL19084
Series:
GSE60449
Download data: GPR
Sample
Accession:
GSM1480281
ID:
301480281
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