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Items: 3

1.

Profiling differentially expressed earthworm genes in response to TNT exposure.

(Submitter supplied) To understand molecular mechanisms of the chronic, sublethal toxicity of 2,4,6-trinitrotoluene (TNT), a widely used ordnance compound of public concerns, we constructed a microarray consisting of 4,032 cDNA isolated from the earthworm Eisenia fetida using the suppressive subtractive hybridization technique. Worms were exposed to a gradient of TNT-spiked soil for 28 days. Based on the reproduction response to TNT, four treatments, i.e., control, 7, 35 and 139 ppm, were selected for gene expression studies. more...
Organism:
Eisenia fetida
Type:
Expression profiling by array
Platform:
GPL4859
40 Samples
Download data: TIFF
Series
Accession:
GSE7024
ID:
200007024
2.

Earthworm 4K cDNA array version 1.0

(Submitter supplied) 1. A total of 4032 purified cDNA clones were loaded on 384-well plates and completely dried down in a Vacufuge Concentrator 5301 (Eppendorf, Westbury, NY). 2. The dried cDNA was re-suspended in 15 ?̬ of 1??printing buffer (ArrayIt, Sunnyvale, CA). 3. Each clone was spotted twice (i.e., in two super grids) on Ultra GAPS amino silane coated glass slides (Corning, Acton, MA) using 16 pins on a VersArray ChipWriter (Bio-Rad, Hercules, CA). more...
Organism:
Eisenia fetida
1 Series
40 Samples
Download data
Platform
Accession:
GPL4859
ID:
100004859
3.

TNT_35ppm_Rep3 vs TNT_7ppm_Rep4

Organism:
Eisenia fetida
Source name:
mRNA from worm (Rep#3) treated with 35 ppm of TNT labeled with Cyanine-3 (green) (channel 1) mRNA from worm (Rep#4) treated with 7 ppm of TNT labeled with Alex Fluor 647 (red) (channel 2)
Platform:
GPL4859
Series:
GSE7024
Download data: TIFF
Sample
Accession:
GSM161795
ID:
300161795
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