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Items: 3

1.

Next generation sequencing of RNA expression on total splenic B cells from NOD mice fed variable diets

(Submitter supplied) Purpose: The goals of this study is to examine if feeding NOD mice with diets supplemented with SCFAs causes altered gene expression in Splenic B cells Methods: B cell RNA profiles from NOD.LT mice fed fed different diets, a non-purified diet (n=4), a high amylose diet (n=4), a high amylose diet supplemented with acetate (n=4) or a high amylose diet supplemented with butyrate (n=4) were generated by deep sequencing, in triplicate, by Illumina 100 - base HT mode the sequence reads that passed quality filters were summarized into gene models using featureCounts from the Rsubread package, and differential gene expression for the various contrasts between dietary treatments was identified using the EdgeR analysis in R Results: Using an optimized data analysis workflow, we mapped about 20 million sequence reads per sample to the mouse genome (build GRCm38) and identified 16,257 transcripts present in B cells from NOD.LT mice fed different diet. more...
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing
Platform:
GPL21493
16 Samples
Download data: TXT
Series
Accession:
GSE94774
ID:
200094774
2.

Illumina HiSeq 3000 (Mus musculus)

Platform
Accession:
GPL21493
ID:
100021493
3.

HA6

Organism:
Mus musculus
Source name:
HA
Platform:
GPL21493
Series:
GSE94774
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Sample
Accession:
GSM2482759
ID:
302482759
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