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Items: 3

1.

Quantification of differential gene expression by multiplexed targeted resequencing of cDNA

(Submitter supplied) We present an approach for quantification of differential mRNA expression by targeted resequencing of cDNA using single-molecule molecular inversion probes (cDNA-smMIPs), which enable highly multiplexed resequencing of cDNA target regions of ~100 nt and counting of individual molecules. We show that accurate estimates of differential expression can be obtained from molecule counts for hundreds of smMIPs per reaction and that smMIPs are also suitable for quantification of relative gene expression and allele-specific expression. more...
Organism:
Homo sapiens; synthetic construct
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL18573 GPL23058
92 Samples
Download data: TXT
Series
Accession:
GSE94800
ID:
200094800
2.

Illumina NextSeq 500 (Homo sapiens; synthetic construct)

Organism:
Homo sapiens; synthetic construct
6 Series
60 Samples
Download data
Platform
Accession:
GPL23058
ID:
100023058
3.

Sample ERCC2 capture replicate 2 with 10.0 ng cDNA input

Organism:
Homo sapiens; synthetic construct
Source name:
ERCC RNA mixed with PBMC RNA
Platform:
GPL23058
Series:
GSE94800
Download data
Sample
Accession:
GSM2483300
ID:
302483300
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