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Items: 3

1.

Quantification of differential gene expression by multiplexed targeted resequencing of cDNA

(Submitter supplied) We present an approach for quantification of differential mRNA expression by targeted resequencing of cDNA using single-molecule molecular inversion probes (cDNA-smMIPs), which enable highly multiplexed resequencing of cDNA target regions of ~100 nt and counting of individual molecules. We show that accurate estimates of differential expression can be obtained from molecule counts for hundreds of smMIPs per reaction and that smMIPs are also suitable for quantification of relative gene expression and allele-specific expression. more...
Organism:
synthetic construct; Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL23058 GPL18573
92 Samples
Download data: TXT
Series
Accession:
GSE94800
ID:
200094800
2.

Illumina NextSeq 500 (Homo sapiens)

Platform
Accession:
GPL18573
ID:
100018573
3.

Sample K562HEK-5 capture replicate 2 with 10.0 ng cDNA input

Organism:
Homo sapiens
Source name:
K562 and HEK293 cell line
Platform:
GPL18573
Series:
GSE94800
Download data
Sample
Accession:
GSM2483360
ID:
302483360
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