GEO DataSets

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Genome variation profiling by SNP array; SNP genotyping by SNP array; Genome variation profiling by genome tiling array

Platforms:

GPL6982 GPL3448

19 Samples

Download data: GFF, PAIR

(Submitter supplied) Highly specific amplification of complex DNA pools without bias or template-independent products (TIPs) remains a challenge. We have developed a procedure using phi29 DNA polymerase and trehalose and optimized control of amplification to create micrograms of specific amplicons without TIPs from down to sub-femtograms of DNA. The amplicons from 5 ng and 0.5 ng DNA, which were from originally good quality of gDNA (05-050), or partially degraded gDNA (04-018), faithfully demonstrated all previously known heterozygous segmental duplications and deletions (3 Mb to 18 kb) located on chromosome 22 and even a homozygous deletion smaller than 1 kb with high resolution chromosome-wide CGH. more...

Organism:

Homo sapiens

Type:

Genome variation profiling by genome tiling array

Platform:

GPL3448

9 Samples

Download data: GFF, PAIR

(Submitter supplied) Maskless array synthesis [1] was used to construct isothermal arrays. This array contains oligonucleotides that start every 85 bp and tiles the entire nonrepetitive part of human chromosome 22 (human genome build NCBI35/hg17 was utilized). Repetitive elements were identified using RepeatMasker [2] and oligonucleotide probes were designed to represent the nonrepetitive DNA sequence. The oligonucleotides were designed to be of variable length, adjusted to match a target melting temperature of 76°C according to the following formula: Tm = 81.5 + 16.6*(log10([Na+])) + 0.41*(%GC) - 600/length. more...

Organism:

Homo sapiens

4 Series

24 Samples

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