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Items: 4

1.

RNA-seq and in situ Hi-C of primary mouse B cell progenitor populations after activation with LPS

(Submitter supplied) During cellular differentiation, chromosome conformation is intricately remodelled to support the lineage-specific transcriptional programs required for initiating and maintaining lineage identity. When these changes occur in relation to cell cycle, division and time is unclear although it has been proposed they would occur during DNA synthesis 5 or after mitosis. Here we elucidate the chromosome conformational changes in B lymphocytes as they differentiate and massively expand from a naïve, quiescent state into antibody secreting plasma cells. more...
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing; Other
Platform:
GPL19057
20 Samples
Download data: MTX, TSV
Series
Accession:
GSE147497
ID:
200147497
2.

In situ HiC of primary mouse B cell progenitor populations after activation with LPS

(Submitter supplied) Naive resting B-cells were purified from the bone marrow of C57BL/6 mice using flow cytometry sorting. Naive B cells were activated with LPS. Activated but undivided cells were harvested 3 hours, 10 hours or 33 hours after the LPS treatment. The Fluorescent Ubiquitination-based Cell Cycle Indicator (FUCCI) system was also used to isolate imminently dividing B cells specifically in the G1 stage of the cell cycle prior to the S phase.
Organism:
Mus musculus
Type:
Other
Platform:
GPL19057
8 Samples
Download data: MTX
Series
Accession:
GSE147467
ID:
200147467
3.

Illumina NextSeq 500 (Mus musculus)

Platform
Accession:
GPL19057
ID:
100019057
4.

B cell activation 33 hours 1

Organism:
Mus musculus
Source name:
Activated B cells
Platform:
GPL19057
Series:
GSE147467 GSE147497
Download data: MTX
Sample
Accession:
GSM4431516
ID:
304431516
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