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Items: 3

1.

Systematic evaluation of genome-wide methylated DNA enrichment using a CpG island array

(Submitter supplied) Methylated DNA enrichment is a key step in a microarray based genome-wide methylation profiling study, and even for future high-throughput sequencing based methylome analysis. In order to evaluate the sensitivity and accuracy of methylated DNA enrichment, we investigated and optimized a number of important parameters to improve the performance of several enrichment assays, including differential methylation hybridization (DMH), microarray-based methylation assessment of single samples (MMASS), and methylated DNA immunoprecipitation (MeDIP). more...
Organism:
Homo sapiens
Type:
Methylation profiling by array
Platform:
GPL9952
17 Samples
Download data: LSR
Series
Accession:
GSE19974
ID:
200019974
2.

Human 9K CGI Array

(Submitter supplied) Clone:pGEM-5Zf(-)/Primers:5'-CGG CCG CCT GCA GGT CGA CCT TAA- 3';5'-AAC GCG TTG GGA GCT CTC CCT TAA- 3';oligo synthesize/strandedness:Methylation tolerant E.coli strain XLI-BLUE MRF'/Length:760 bp(Average insert size);Arrayer Harderware:Aminosilane Coated Glass Slides/Software:Arrays were scanned with a confocal LuxScanTM scanner (CapitalBio Corp.), and obtained images were analyzed with LuxScan3.0 TM software (CapitalBio Corp);Spotting protocol:The PCR products were precipitated with ethanol and re-dissolved in EasyArrayTM Spotting Solution (CapitalBio Corp.), then printed in triplicate on Aminosilane Coated Glass Slides using a SmartArrayTM microarrayer (All from CapitalBio Corp.).
Organism:
Homo sapiens
1 Series
17 Samples
Download data: GAL
Platform
Accession:
GPL9952
ID:
100009952
3.

MeDIP 1st antibody incubation time 12h, 2nd antibody incubation time 1h - 803

Organism:
Homo sapiens
Source name:
Input (methylated + unmethylated) DNA of gastric adenocarcinoma cell line MGC-803 (channel 1) IP (methylated) DNA of gastric adenocarcinoma cell line MGC-803 (channel 2)
Platform:
GPL9952
Series:
GSE19974
Download data: LSR
Sample
Accession:
GSM499098
ID:
300499098
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