GEO DataSets

(Submitter supplied) Background: Most asthmatic patients have high serum levels of IgE directed against common environmental allergens such as house dust mite, animal danders and moulds. However the presence of specific IgE against individual allergens alone does not account for asthma. Many individuals are atopic but not asthmatic. Precise knowledge of the serum IgE specificity repertoire in asthmatic and non-asthmatic patients would substantially help in understanding the pathogenesis of the disease and at the same time facilitate the treatment and the implementation of preventive measures. more...

Organism:

Dermatophagoides pteronyssinus; Homo sapiens

Type:

Protein profiling by protein array

Platform:

GPL9968

872 Samples

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(Submitter supplied) The array contained 103 allergens printed in duplicate onto aldehyde-activated glass microscope slides (CEL Associates) using high-speed robotics (Microgrid Compact; Biorobotics). Arrays were printed at 23°C/60°C humidity and stored overnight inside the printing cabinet. Allergens (provided from Allergopharma) were initially reconstituted in PBS pH7.4 (reconstitution buffer) with a final concentration ranging form 0.4 to 40 mg/ml and after that spotted onto the arrays in the following spotting buffer PBS pH7.4, glycine pH2.4, Borate pH9.4, glycerol 10%, DTT 5mM, SDS (0.2%; 0.05%), Tween 20 (0.01%). more...

Organism:

Dermatophagoides pteronyssinus

1 Series

872 Samples

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