GEO DataSets

(Submitter supplied) The model prokaryote Escherichia coli can exist as a either a commensal or a pathogen in the gut of diverse mammalian hosts. These associations, coupled with its ease of cultivation and genetic variability, have made E. coli a popular indicator organism for tracking the origin of fecal water contamination. Source tracking accuracy is predicated on the assumption that E. coli isolates recovered from contaminated water present a genetic signature characteristic of the host from which they originated. more...

Organism:

Escherichia coli; Escherichia coli str. K-12 substr. MG1655

Type:

Genome variation profiling by array

Platform:

GPL8872

24 Samples

Download data: GPR

(Submitter supplied) Corning Gaps II aminosilane coated slides Protocol: Microarrays were produced using full-length open reading frame PCR products generated with the Sigma-Genosys ORFmers primer set and reaction conditions and cycling parameters recommended by the manufacturer (Sigma-Genosys, The Woodlands, TX). This set contains primer pairs for all 4290 known and hypothetical ORFs in E. coli K12 MG1655. PCR reactions were repeated and pooled as necessary to obtain at least 3 micrograms of DNA and pooled reactions were ethanol precipitated, resuspended and further purified using a Qiagen MinElute96 UF PCR purification kit. more...

Organism:

Escherichia coli str. K-12 substr. MG1655

4 Series

69 Samples

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