GSM86391[Accession] - GEO DataSets

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Items: 3

Select item 2000037581.

Interactions between Saccharomyces cerevisiae WW domains and proteins discovered using protein microarrays

(Submitter supplied) A total of 12 WW domains from Saccharomyces cerevisiae were expressed and purified as fusion proteins to either GST or MBP. The fusion proteins were chemically biotinylated and applied to duplicate protein microarrays. Data processing revealed a total of 587 interactions between the domains and 207 proteins. Most of these interactions have not been previously observed. Keywords: Protein microarray analysis of yeast WW domains

Organism:: Saccharomyces cerevisiae

Type:: Protein profiling by protein array

Platform:: GPL3234
24 Samples

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Accession:: GSE3758

ID:: 200003758

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 1000032342.

Invitrogen Yeast Protoarray

(Submitter supplied) The Yeast ProtoArray™ high-density functional protein microarray is the first microarray product that enables rapid elucidation of proteinprotein interactions on a proteome scale. The Yeast ProtoArray™ contains 4,088 S. cerevisiae open reading frames (ORFs) expressed as 5'-GST fusions, purified and spotted in duplicate on a 1 inch x 3 inch nitrocellulose-coated slide (Figure 1).With the Yeast ProtoArray™ PPI Kit, you can identify novel protein interactions in a single day, instead of the several weeks required using alternatives such as yeast two-hybrid systems. more...