GEO DataSets

(Submitter supplied) In a time course study, we characterized global gene expression profile of B. melitensis infection in bovine Peyer patches in the first 4 h p.i. Statistical analysis of microarray results reveal that 2,356 B. melitensis genes were detected as differentially expressed (z-score p < 0.025) compared with control between 15 min and 4 h post-infection. A group of 1,740 genes that were differentially expressed in at least 4 out of 5 time points was considered the core set of genes that reflect the major changes in B. more...

Organism:

Brucella melitensis; Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL7612

44 Samples

Download data: GPR, TXT

(Submitter supplied) A whole genome expression comparision between wild type B. melitensis 16M and ΔBM-LOV-HK (ΔBMEII0679) B. melitensis strains grown in broth culture to determine genes directly and indirectly regulated by BM-LOV-HK.

Organism:

Brucella melitensis bv. 1 str. 16M; Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL16337

6 Samples

Download data: CALLS, PAIR

(Submitter supplied) Hfq is a bacterial Sm-like protein which serves as a post-transcriptional regulator of global gene expression. It has been shown to be involved in a wide range of cellular processes and pathways. To characterize the regulation roles of Hfq in Brucella melitensis, the in vitro hfq induction conditions simulating intracellular environments were firstly defined, and then, the transcriptome of the hfq mutant was compared to that of the wild type strain. more...

Organism:

Brucella melitensis; Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL11117

4 Samples

Download data: GPR

(Submitter supplied) The four-carbon sugar erythritol is an important component of B. melitensis pathogenesis. To determine the transcriptional response to erythritol, B. melitensis strain 16M was grown in the presence of either glucose or erythritol as a sole carbon source.

Organism:

Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL15533

4 Samples

Download data: CALLS, PAIR

(Submitter supplied) Investigation of whole genome gene expression level changes in a Brucella melitensis delta prlr mutant compared to the wild type strain. The mutants analyzed in this study are further described in A. Mirabella, R-M Yanez, R.M. Delrue, S. Uzureau, M.S. Zygmunt, A. Cloeckaert, X. De Bolle, J.J. Letesson (2012). The two component system PrlS/PrlR of Brucella melitensis is required for persistence in mice and appears to respond to ionic strength. more...

Organism:

Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL5730

6 Samples

Download data: PAIR

(Submitter supplied) Brucella spp. is an intracellular pathogen in vivo. The intracellular B. melitensis transcriptome was determined by initially enriched and then amplified B. melitensis RNA from total RNA of B. melitensis-infected HeLa cells. Analysis of microarray results identified 161 and 115 genes differentially expressed at 4 and 12 h p.i., respectively. Most of the genes (78%) differentially expressed were down-regulated at the earliest time point, but up-regulated (75%) at 12 h p.i. more...

Organism:

Brucella melitensis; Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL8156

18 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Brucella ovis; Brucella melitensis; Brucella canis; Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platforms:

GPL15024 GPL15023

16 Samples

Download data: CALLS, PAIR

(Submitter supplied) Brucella melitensis and Brucella canis differ by ~75 genes yet B. melitensis is highly virulent for humans while B. canis is considered rarely pathogenic. No identified bacterial factors or mechanisms account for this difference in virulence. To identify functional differences of these two bacteria, gene transcription was examined during infection of murine macrophages and compared to bacteria grown in broth. more...

Organism:

Brucella melitensis bv. 1 str. 16M; Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL15023

4 Samples

Download data: CALLS, PAIR

(Submitter supplied) Quorum sensing system (QS) is a global regulator that regulates gene expression in response to cell density and other environmental factors. Two QS genes have been identified in Brucella, one of which (vjbR) is involved in Brucella intracellular survival and virulence. But the regulation mechanism of Brucella intracellular survival by the vjbR remains unknown.To characterize the regulation roles of vjbR, the in vitro vjbR induction conditions simulating intracellular environments were firstly defined, and then, transcriptome of the vjbR mutant is compared to that of the wild type strain. more...

Organism:

Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL11117

4 Samples

Download data: GPR

(Submitter supplied) The transcriptional regulator MucR was identified as highly attenuated in a mutagenesis screen conducted by this laboratory. MucR has been described as a key regulator of exopolysaccharide synthesis in closely related Rhizobials soil symbionts. The goal of this study was to apply custom Brucella melitensis oligonucleotide microarrays to identify the regulatory targets of MucR. These studies revealed that MucR's regulation of exopolysaccharide synthesis is also conserved in Brucella melitensis and is also a key regulator of iron sequestering/storage and denitrification. more...

Organism:

Brucella melitensis bv. 1 str. 16M; Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL7612

6 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Brucella melitensis; Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL7612

30 Samples

Download data: GPR

(Submitter supplied) Quorum sensing (QS) is a communication system in bacteria that regulates gene expression in response to a small diffusible signal indicative of the bacterial population present. QS has been shown to regulate virulence genes, biofilm formation, cell division and secretion systems in a diverse set of bacteria. Brucella melitensis produces a QS signaling molecule, N-dodecanoyl homoserine lactone (C12-HSL), that interacts with a LuxR transcriptional regulator, VjbR. more...

Organism:

Brucella melitensis bv. 1 str. 16M; Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL7612

12 Samples

Download data: GPR

(Submitter supplied) Quorum sensing (QS) is a communication system in bacteria that regulates gene expression in response to a small diffusible signal indicative of the bacterial population present. QS has been shown to regulate virulence genes, biofilm formation, cell division and secretion systems in a diverse set of bacteria. Brucella melitensis produces a QS signaling molecule, N-dodecanoyl homoserine lactone (C12-HSL), that interacts with a LuxR transcriptional regulator, VjbR. more...

Organism:

Brucella melitensis; Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL7612

18 Samples

Download data: GPR

(Submitter supplied) Many pathogenic bacteria use a regulatory process termed Quorum Sensing (QS) to produce and detect small diffusible molecules to synchronize gene expression within a population. In Gram-negative bacteria, the detection and response to these molecules depend on transcriptional regulators belonging to the LuxR family. Such a system have been discovered in the intracellular pathogen Brucella melitensis, a Gram-negative bacteria responsible for brucellosis, a word-wide zoonosis remaining a serious public health concern in endemic countries. more...

Organism:

Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL5730

3 Samples

Download data: FTR

(Submitter supplied) NimbleGen design name BMEL-00001-X. The 3198 ORFs of B. melitensis are represented by seventeen 24-mer probe pairs (PM/MM) per gene. Protocol: High-density DNA array prepared with Maskless Array Synthesizer (MAS) technology. See manufacturer's website at http://www.nimblegen.com/.

Organism:

Brucella melitensis bv. 1 str. 16M

1 Series

4 Samples

Download data: NDF, TXT

(Submitter supplied) A set of unique 70-base oligonucleotides representing 3,227 ORFs of B. melitensis strain 16M and unique / divergent genes from B. abortus and B. suis were designed at the Pathogen Expression Core and purchased from Sigma-Genosys (The Woodland, TX). Oligonucleotides were suspended in 3X SSC (Ambion) at the final concentration of 40 uM before robotic arraying in triplicate onto ultraGAPS coated glass slides (Corning) using a Spotarray 72 microarray printer (Perkin Elmer, Downer’s Grove, ILL). more...

Organism:

Brucella melitensis bv. 1 str. 16M

1 Series

18 Samples

Download data

(Submitter supplied) 1.Primers specifc for each of the ORFs of 16M genome sequence are designed by Arraydesigner 3.0. Primers are synthesized in 96-well plates. 2. Products of each of the ORFs were amplified in 96-well plate. 3. PCR products are purified in 96-well plates. 4. PCR products are disolved in 50% DMSO, gyrB, glk and 16s rRNA are included in each of the blocks as positive contrl, and 50% DMSO as negative control. more...

Organism:

Brucella melitensis bv. 1 str. 16M

2 Series

8 Samples

Download data

(Submitter supplied) The design (.ndf) file and description (.ngd) file are available as Supplementary files (at the foot of this record). Protocol: Microarray design and experimentation were made by NimbleGen Systems, INC from catalogue design for B. melitensis 16M chromosomes I (NC_003317) and II (NC_003318) with 20 probes per gene (10 perfect matches and 10 mismatches). Each probe (24 mer) was replicated three times on chip (design includes random GC probes). more...

Organism:

Brucella melitensis bv. 1 str. 16M

2 Series

9 Samples

Download data: NDF, NGD