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Items: 1 to 20 of 160

1.

Discovery and characterization of the first known biological lanthanide chelator

(Submitter supplied) Many bacteria secrete metallophores, low-molecular weight organic compounds that bind ions with high selectivity and affinity, in order to access essential metals from the environment. The biosynthetic machinery to produce metallophores as well as their structures have been elucidated for iron, zinc, nickel, molybdenum, and copper specific molecules. No lanthanide-specific metallophore has been discovered despite the knowledge that lanthanide metals (Ln) have recently been revealed to be essential cofactors for certain alcohol dehydrogenases across a diverse range of phyla. more...
Organism:
Methylorubrum extorquens AM1
Type:
Expression profiling by high throughput sequencing
Platform:
GPL33789
9 Samples
Download data: CSV
Series
Accession:
GSE244060
ID:
200244060
2.

Improvement of dicarboxylic acid production with Methylorubrum extorquens by reduction of product reuptake

(Submitter supplied) In the past, two dicarboxylic acid products, namely mesaconic acid and 2‑methylsuccinic acid, were successfully produced by Methylorubrum extorquens AM1 with heterologous thioesterase YciA, but the yield was reduced by product reuptake. In our study we conducted comprehensive research on the uptake mechanism of those dicarboxylic acid products. To identify potential import factors, we performed transcriptome analysis of a strain harboring plasmid pCM160_RBS_yciAHI and an empty vector control strain. more...
Organism:
Methylorubrum extorquens AM1
Type:
Expression profiling by high throughput sequencing
Platform:
GPL32105
30 Samples
Download data: TXT, XLSX
Series
Accession:
GSE199961
ID:
200199961
3.

Hyperaccumulation of gadolinium by Methylorubrum extorquens AM1 reveals impacts of lanthanides on cellular processes beyond methylotrophy

(Submitter supplied) We report a genetic variant of Methylorubrum extorquens AM1 that hyperaccumulates the heavy lanthanide gadolinium. Using RNA-seq transcriptomics we identified wide-spread metabolic and physiological changes in this strain and experimentally validate several of them, including increased gadolinium transport and storage in an intracellular compartment we name the lanthasome.
Organism:
Methylorubrum extorquens AM1
Type:
Expression profiling by high throughput sequencing
Platform:
GPL31182
8 Samples
Download data: TXT
Series
Accession:
GSE193171
ID:
200193171
4.

Contrasting in vitro and in vivo methanol oxidation activities of lanthanide-dependent alcohol dehydrogenases XoxF1 and ExaF from Methylobacterium extorquens AM1

(Submitter supplied) We report gene expression profiles for cultures grown in minimal salts medium with 125 mM methanol with and without addition of 2 uM lanthanum chloride
Organism:
Methylorubrum extorquens AM1
Type:
Expression profiling by high throughput sequencing
Platform:
GPL26093
5 Samples
Download data: TXT
Series
Accession:
GSE125593
ID:
200125593
5.

The Epigenomic Landscape of Prokaryotes

(Submitter supplied) DNA methylation is an important regulator of genome function in the eukaryotes, but it is currently unclear if the same is true in prokaryotes. While regulatory functions have been demonstrated for a small number of bacteria, there have been no large-scale studies of prokaryotic methylomes and the full repertoire of targets and biological functions of DNA methylation remains unclear. Here we applied single-molecule, real-time sequencing to directly study the methylomes of 232 phylogenetically diverse prokaryotes. more...
Organism:
Lactococcus lactis subsp. lactis; Lactiplantibacillus plantarum; Lachnobacterium bovis; Clostridium perfringens ATCC 13124; Methanocaldococcus jannaschii DSM 2661; Methylorubrum extorquens AM1; Thermoplasma volcanium GSS1; Acidobacteriaceae bacterium TAA 166; Mycoplasmopsis bovis PG45; Methanospirillum hungatei JF-1; Actinobacillus succinogenes 130Z; Fervidobacterium nodosum Rt17-B1; Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088; Staphylothermus marinus F1; Thermoanaerobacter sp. X514; Xenorhabdus nematophila ATCC 19061; Galbibacter orientalis; Dyadobacter fermentans DSM 18053; Streptosporangium roseum DSM 43021; Pedobacter heparinus DSM 2366; Rhizobium etli CIAT 652; Meiothermus ruber DSM 1279; Planctopirus limnophila DSM 3776; Methanothermus fervidus DSM 2088; Sebaldella termitidis ATCC 33386; Methanohalophilus mahii DSM 5219; Aminobacterium colombiense DSM 12261; Acidobacteriaceae bacterium KBS 146; Pontibacter actiniarum DSM 19842; Thermobacillus composti KWC4; Marinithermus hydrothermalis DSM 14884; Bernardetia litoralis DSM 6794; Desulfobacca acetoxidans DSM 11109; Rikenella microfusus DSM 15922; Echinicola vietnamensis DSM 17526; Orenia marismortui DSM 5156; Sporocytophaga myxococcoides DSM 11118; Niabella soli DSM 19437; Sinorhizobium medicae WSM1115; Hippea alviniae EP5-r; Hippea sp. KM1; Sphingomonas melonis C3; Methylophilaceae bacterium 11; Thioalkalivibrio sp. ARh3; Thiomonas sp. FB-6; Oxalobacteraceae bacterium AB_14; Solidesulfovibrio cf. magneticus IFRC170; Desulfotignum balticum DSM 7044; Methylobacterium sp. EUR3 AL-11; Kallotenue papyrolyticum; Bryobacter aggregatus MPL3; Ruminococcus albus AD2013; Eubacterium sp. AB3007; Ruminococcaceae bacterium AE2021; Lachnospiraceae bacterium AC2031; Selenomonas ruminantium AC2024; Peptostreptococcaceae bacterium VA2; Ruminococcus sp. HUN007; Streptococcus equinus; Salmonella enterica subsp. arizonae serovar 62:z4,z23:-; Xylella fastidiosa Temecula1; Acetivibrio thermocellus ATCC 27405; Rhodopseudomonas palustris CGA009; Neisseria meningitidis FAM18; Thermoplasma acidophilum DSM 1728; Hydrogenovibrio crunogenus XCL-2; Chloroflexus aggregans DSM 9485; Thermosipho melanesiensis BI429; Shewanella woodyi ATCC 51908; Bradyrhizobium elkanii USDA 76; Dinoroseobacter shibae DFL 12 = DSM 16493; Parabacteroides distasonis ATCC 8503; Anoxybacillus flavithermus WK1; Escherichia coli str. K-12 substr. MG1655; Capnocytophaga ochracea DSM 7271; Haloterrigena turkmenica DSM 5511; Palaeococcus ferrophilus DSM 13482; Acetivibrio thermocellus DSM 1313; Gracilinema caldarium DSM 7334; Treponema succinifaciens DSM 2489; Caldithrix abyssi DSM 13497; Calidithermus chliarophilus DSM 9957; Cohnella panacarvi Gsoil 349; Methylobacterium sp. 10; Xanthobacter sp. 91; Geopsychrobacter electrodiphilus DSM 16401; Hydrogenovibrio marinus DSM 11271; Nocardia sp. BMG111209; Klebsiella oxytoca BRL6-2; Polaribacter sp. Hel_I_88; Methylohalobius crimeensis 10Ki; Streptomyces sp. WMMB 714; Ruminiclostridium josui JCM 17888; Alteromonas sp. ALT199; Aminiphilus circumscriptus DSM 16581; Caldicoprobacter oshimai DSM 21659; Microbacterium sp. KROCY2; Thermogemmatispora carboxidivorans; Ruminococcus flavefaciens AE3010; Selenomonas ruminantium AB3002; Butyrivibrio sp. FCS014; Polycyclovorans algicola TG408; Clostridium sp. KNHs205; Lachnospiraceae bacterium AC2029; Enterococcus faecalis 68A; Butyrivibrio sp. AE3004; Teredinibacter purpureus; Teredinibacter turnerae; Escherichia coli CFT073; Salmonella bongori NCTC 12419; Treponema denticola ATCC 35405; Akkermansia muciniphila ATCC BAA-835; Phaeobacter inhibens DSM 17395; Actinosynnema mirum DSM 43827; Staphylococcus aureus subsp. aureus USA300_TCH1516; Sphaerobacter thermophilus DSM 20745; Veillonella parvula DSM 2008; Streptobacillus moniliformis DSM 12112; Allomeiothermus silvanus DSM 9946; Sedimentitalea nanhaiensis DSM 24252; Sediminispirochaeta smaragdinae DSM 11293; Hirschia baltica ATCC 49814; Coraliomargarita akajimensis DSM 45221; Syntrophothermus lipocalidus DSM 12680; Stutzerimonas stutzeri RCH2; Syntrophobotulus glycolicus DSM 8271; Bacillus spizizenii str. W23; Phocaeicola salanitronis DSM 18170; Pseudofrankia sp. DC12; Nitratifractor salsuginis DSM 16511; Cellulophaga lytica DSM 7489; Asinibacterium sp. OR53; Solitalea canadensis DSM 3403; Patulibacter minatonensis DSM 18081; Acetobacterium woodii DSM 1030; Nocardia sp. BMG51109; Halomicrobium katesii DSM 19301; Nitriliruptor alkaliphilus DSM 45188; Methylophilus sp. 1; Pseudomonas aeruginosa NCAIM B.001380; Kangiella aquimarina DSM 16071; Pelobacter seleniigenes DSM 18267; Thiomicrospira pelophila DSM 1534; Desulfurobacterium sp. TC5-1; Bacteroides sp. 14(A); Clostridium sp. 12(A); Hydrogenovibrio kuenenii DSM 12350; Leptolyngbya sp. PCC 6406; Maribacter sp. Hel_I_7; Desulfospira joergensenii DSM 10085; Tolumonas lignilytica; Cellvibrionaceae bacterium 1162T.S.0a.05; [Clostridium] methoxybenzovorans SR3; [Clostridium] indolis DSM 755; Desulforegula conservatrix Mb1Pa; Oceanicola sp. HL-35; Algoriphagus marincola HL-49; Desulfohalovibrio reitneri; Alicyclobacillus macrosporangiidus CPP55; Pseudacidobacterium ailaaui; Mediterraneibacter gnavus AGR2154; Sediminibacter sp. Hel_I_10; Hydrogenovibrio sp. MA2-6; Pseudobutyrivibrio ruminis HUN009; Lachnoclostridium phytofermentans KNHs212; Robinsoniella sp. KNHs210; Enterococcus gallinarum; Clostridium algidicarnis; Pyrococcus horikoshii OT3; Methylocystis sp. LW5; Agrobacterium fabrum str. C58; Persephonella; Mastigocladopsis repens PCC 10914; Neisseria gonorrhoeae FA 1090; Clostridioides difficile 630; Thiobacillus denitrificans ATCC 25259; Salmonella enterica subsp. enterica serovar Paratyphi A str. ATCC 9150; Sulfurimonas denitrificans DSM 1251; Sulfolobus acidocaldarius DSM 639; Flavobacterium psychrophilum JIP02/86; Methanocorpusculum labreanum Z; Cronobacter; Pseudarthrobacter chlorophenolicus A6; Saccharomonospora viridis DSM 43017; Verrucomicrobia bacterium LP2A; Thermanaerovibrio acidaminovorans DSM 6589; Corynebacterium aurimucosum ATCC 700975; Zymomonas mobilis subsp. pomaceae ATCC 29192; Klebsiella aerogenes FGI35; Cellulophaga algicola DSM 14237; Flexistipes sinusarabici DSM 4947; Sulfurospirillum barnesii SES-3; Gillisia limnaea DSM 15749; Spirochaeta thermophila DSM 6578; Ruminococcus sp. NK3A76; Spirochaeta africana DSM 8902; Holophaga foetida DSM 6591; Salmonella enterica subsp. enterica serovar Paratyphi B str. SPB7; Acetivibrio clariflavus 4-2a; Thermacetogenium phaeum DSM 12270; Methylophilus sp. 5; Arthrobacter sp. 31Y; Methylophilus sp. 42; Methylotenera versatilis 79; Psychrilyobacter atlanticus DSM 19335; Prevotella sp. 10(H); Methylotenera sp. 73s; Acidovorax sp. JHL-3; Gillisia sp. JM1; Cellulomonas sp. KRMCY2; Clostridium sp. ASBs410; Limisalsivibrio acetivorans; Polaromonas sp. EUR3 1.2.1; Levilactobacillus brevis AG48; Pediococcus acidilactici AGR20; Exiguobacterium chiriqhucha; Prevotella sp. HUN102; Flavimarina sp. Hel_I_48; Lachnospiraceae bacterium AC2012; Clostridioides mangenotii LM2; Exiguobacterium aurantiacum DSM 6208; Exiguobacterium acetylicum DSM 20416; Exiguobacterium oxidotolerans JCM 12280; Exiguobacterium antarcticum DSM 14480; Methylobacter tundripaludum 21/22; Lachnoclostridium phytofermentans KNHs2132; Staphylococcus epidermidis AG42; Butyrivibrio sp. AE3003
Type:
Methylation profiling by high throughput sequencing
228 related Platforms
237 Samples
Download data: CSV, GFF
Series
Accession:
GSE69872
ID:
200069872
6.

Formaldehyde stress response in Methylobacterium extorquens AM1

(Submitter supplied) The goal of this study was to use microarrays to identify genes differentially regulated under conditions of formaldehyde stress relative to two other stress conditions (oxidative, osmotic) in an effort to identify genes that might be involved in a formaldehyde-specific stress response, rather than a general stress response, in the model methylotroph Methylobacterium extorquens AM1.
Organism:
Methylorubrum extorquens AM1
Type:
Expression profiling by array
Platform:
GPL6262
18 Samples
Download data: TXT
Series
Accession:
GSE15631
ID:
200015631
7.

Engineered Methylobacterium methanol

(Submitter supplied) Engineered Methylobacterium methanol batch exponential
Organism:
Methylorubrum extorquens AM1
Type:
Expression profiling by array
Platform:
GPL6262
1 Sample
Download data: TXT
Series
Accession:
GSE34662
ID:
200034662
8.

Ethylmalonyl-CoA mutase Operates as a Metabolic Control Point in Methylobacterium extorquens AM1

(Submitter supplied) We report RNA-seq datasets profiling the transcriptional response to a sudden change in growth substrate, from succinate to ethylamine. This detailed combined dataset provides a dynamic assessment of the transcriptional response to a metabolic perturbation. These datasets are the first reported RNA-seq datasets for gene expression in Methylobacterium extorquens AM1
Organism:
Methylorubrum extorquens AM1
Type:
Expression profiling by high throughput sequencing
Platform:
GPL19397
12 Samples
Download data: TXT
Series
Accession:
GSE63115
ID:
200063115
9.

Restoration and reinforcement of acclimatizing responses during the adaptation of an unfit, metabolically engineered strain

(Submitter supplied) Organisms cope with physiological stressors through acclimatizing mechanisms in the short-term, and through adaptive mechanisms over evolutionary timescales. Whereas the former offer a consistent and largely predictable buffer against stressors, myriad paths of adaptation are often possible. Our work examined whether knowledge of acclimatizing responses could be informative of  aspects of future adaptation, using as a model system a strain of Methylobacterium extorquens AM1 that was experimentally engineered and then evolved with a novel central metabolism. more...
Organism:
Methylorubrum extorquens AM1
Type:
Expression profiling by array
Platform:
GPL16255
30 Samples
Download data: TXT
Series
Accession:
GSE42116
ID:
200042116
10.

Transition from succinate to methanol growth in Methylobacterium Extorquens AM1

(Submitter supplied) In order to provide information about the gene expression response that occurs when cells experience a change in carbon source, succinate limited chemostat cultures of Methylobacterium extorquens AM1 were grown to and maintained at an OD of ~0.63, transferred to flasks and methanol was added. Cells were harvested for RNA extraction at time: 0 min, 10 min, 30 min, 1 hr, 2 hr, 4 hr and 6 hr post transition. more...
Organism:
Methylorubrum extorquens AM1
Type:
Expression profiling by array
Platform:
GPL6262
24 Samples
Download data: TXT, XLS
Series
Accession:
GSE22031
ID:
200022031
11.

Ethylamine versus succinate cells of Methylobacterium extorquens

(Submitter supplied) In order to provide global information on gene expression during growth on C2 compounds, microarray analysis of M. extorquens AM1 cells was carried out, comparing ethylamine-grown cells to succinate-grown cells. This comparison has confirmed previous observations on the inducible nature of some of the enzymes involved in C2 metabolism, such as methylamine (and ethylamine) utilization system (mau), putative enzymes for converting acetaldehyde into acetate and acetyl-CoA, and enzymes of the ethylmalonyl-CoA pathway that has been proposed to operate for assimilation of acetyl-CoA into cell biomass.
Organism:
Methylorubrum extorquens AM1; Methylorubrum extorquens
Type:
Expression profiling by array
Platforms:
GPL6262 GPL10058
4 Samples
Download data: TXT
Series
Accession:
GSE20365
ID:
200020365
12.

Fast Growth Increases the Selective Advantage of a Mutation Arising Recurrently during Evolution under Metal Limitation

(Submitter supplied) The goal was to use microarray to compare changes in the global transcription profiles between an engineered bacterial strain and one of its descendants subject to 600 generations of experimental evolution in batch culture. This comparison allowed us to identify a beneficial mutation that substantially increased expression of a novel cobalt transporter cassette in this descendant.
Organism:
Methylorubrum extorquens AM1
Type:
Expression profiling by array
Platform:
GPL6262
1 Sample
Download data: TXT
Series
Accession:
GSE14875
ID:
200014875
13.

Illumina NovaSeq 6000 (Methylorubrum extorquens AM1)

Organism:
Methylorubrum extorquens AM1
1 Series
9 Samples
Download data
Platform
Accession:
GPL33789
ID:
100033789
14.

Illumina NextSeq 500 (Methylorubrum extorquens AM1)

Organism:
Methylorubrum extorquens AM1
1 Series
30 Samples
Download data
Platform
Accession:
GPL32105
ID:
100032105
15.

Illumina MiSeq (Methylorubrum extorquens AM1)

Organism:
Methylorubrum extorquens AM1
1 Series
8 Samples
Download data
Platform
Accession:
GPL31182
ID:
100031182
16.

Illumina HiSeq 2500 (Methylorubrum extorquens AM1)

Organism:
Methylorubrum extorquens AM1
1 Series
5 Samples
Download data
Platform
Accession:
GPL26093
ID:
100026093
17.

PacBio RS II (Methylobacterium extorquens AM1)

Organism:
Methylorubrum extorquens AM1
1 Series
1 Sample
Download data
Platform
Accession:
GPL20450
ID:
100020450
18.

Illumina HiSeq 2000 (Methylobacterium extorquens AM1)

Organism:
Methylorubrum extorquens AM1
1 Series
12 Samples
Download data
Platform
Accession:
GPL19397
ID:
100019397
19.

Marx lab 2012 Harvard University custom Agilent 60-mer array with 15K+ features for Methylobacterium extorquens AM1

(Submitter supplied) see manufacturer's website
Organism:
Methylorubrum extorquens AM1
1 Series
30 Samples
Download data
Platform
Accession:
GPL16255
ID:
100016255
20.

Agilent-019314 UW_Lidstrom_2008 Methylobacterium extorquens AM1 11k array

(Submitter supplied) Methylobacterium extorquens strain AM1 Platform annotation based on June 2002 annotation (genome sequencing done by Integrated Genomics, http://www.integratedgenomics.com/genomereleases.html). Protocol: See manufacturer's web site at http://www.agilent.com/
Organism:
Methylorubrum extorquens AM1
1 Series
2 Samples
Download data
Platform
Accession:
GPL10058
ID:
100010058
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