GEO DataSets

(Submitter supplied) We examined gene expression differences associated with changes in light cycle in Drosophila montana

Organism:

Drosophila montana

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18881

12 Samples

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(Submitter supplied) Most northern insect species experience a period of developmental arrest, diapause, which enables them to survive over the winter and postpone reproduction until favorable conditions. We studied the timing of reproductive diapause and its long-term effects on the cold tolerance of Drosophila montana, D. littoralis and D. ezoana females in seasonally varying environmental conditions. At the same time we traced expression levels of 219 genes in D. more...

Organism:

Drosophila virilis; Drosophila montana

Type:

Expression profiling by array

Platform:

GPL14008

23 Samples

Download data: TXT

(Submitter supplied) For many organisms the ability to cold acclimate with the onset of seasonal cold has major implications for their fitness. In insects, where this ability is widespread, the physiological changes associated with increased cold tolerance have been well studied. Despite this, little work has been done to trace changes in gene expression during cold acclimation that lead to an increase in cold tolerance. more...

Organism:

Drosophila virilis; Drosophila montana

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL18882 GPL18881

10 Samples

Download data: TXT

(Submitter supplied) We utilized a candidate gene approach using custom microarray constructed for our study species Drosophila montana and D. virilis to identify genes with modulated expression patterns under low temperature conditions. The flies were exposed to four different treatments (+5°C for 6 days, 0°C for 1 hour, two periods of recovery after cold stress and a control treatment). The aim of the study was to identify potential cold-responsive genes and to investigate differences in gene expression between the species. more...

Organism:

Drosophila montana; Drosophila virilis

Type:

Expression profiling by array

Platform:

GPL14008

24 Samples

Download data: TXT

(Submitter supplied) We created a custom-made microarray for D. montana with 101 genes known to affect traits important in diapause, photoperiodism, reproductive behaviour, circadian clock and stress tolerance in model Drosophila species. This array gave us a chance to filter out genes showing expression changes during photoperiodic reproductive diapause in a species adapted to live in northern latitudes with high seasonal changes.

Organism:

Drosophila montana

Type:

Expression profiling by array

Platform:

GPL9293

9 Samples

Download data: TXT

(Submitter supplied) The array contains multiple probes per a particular gene, designed separately for the two study species, using species-specific sequences (either D.montana or D. virilis). Protocol: Since there is no genome sequence available for D. montana (and only partial annotations for D. virilis genome), we selected genes on the array from the D. melanogaster genome using FlyBase gene ontology searches on biological response of interest (http://flybase.org/). more...

Organism:

Drosophila virilis; Drosophila montana

2 Series

47 Samples

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(Submitter supplied) The primers for the candidate genes of D. montana were designed for one to three unique 200-500 bp regions with Primer3 program (http://www-genome.wi.mit.edu/genome software/other/ primer3.html) or manually, using information on the gene alignments mentioned above. Next, an online program e-Array 5.3 (Agilent) was used to design one to four 60 bp oligonucleotide probes per gene using exon regions. Finally, the probes were synthesized in situ with liquid chemistry and arrayed on an “Agilent 60-mer Multi-Pack Gene Expression Microarray” platform with one-colour system. more...

Organism:

Drosophila montana

1 Series

9 Samples

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