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Items: 1 to 20 of 1831

1.

A potent HDAC inhibitor blocks Toxoplasma gondii tachyzoite growth and profoundly disrupts parasite gene expression.

(Submitter supplied) Toxoplasmosis is a major health issue worldwide especially for immune-deficient individuals and the offspring of newly infected mothers. It is caused by a unicellular intracellular parasite called Toxoplasma gondii. Although the drugs commonly used to treat toxoplasmosis are efficient, they present serious side effects and adverse events are common. Therefore, there is a need for the discovery of new compounds with potent anti-T. more...
Organism:
Toxoplasma gondii
Type:
Expression profiling by high throughput sequencing
Platform:
GPL23377
6 Samples
Download data: XLSX
Series
Accession:
GSE175919
ID:
200175919
2.

Spermine-induced DNA methylation change in human macrophages

(Submitter supplied) Polyamines, crucial molecules involved in cell proliferation and growth, play a pivotal role in cancer development and progression. Within the tumor microenvironment, macrophages, key components of the immune system, exhibit a complex relationship with polyamines. Evidence suggests that polyamines can modulate macrophage polarization, influencing their functional phenotypes. Here, we detected the gene DNA methylation changes in spermine-stimulated human macrophages isolated from PBMCs and TAMs.
Organism:
Yersinia pseudotuberculosis; Rickettsia prowazekii; Bartonella quintana; Mycobacterium avium; Homo sapiens; Streptobacillus moniliformis; Bartonella henselae; Francisella tularensis subsp. tularensis; Francisella tularensis subsp. holarctica; Leptospira interrogans; Rickettsia typhi; Mycobacterium tuberculosis variant bovis; Mycobacterium tuberculosis; Mycobacterium tuberculosis variant microti; Mycobacterium canetti; Orthohantavirus seoulense; Yersinia enterocolitica; Toxoplasma gondii; Salmonella enterica subsp. enterica serovar Typhimurium; Mammarenavirus choriomeningitidis; Orthohantavirus puumalaense; Campylobacter jejuni; Francisella tularensis subsp. novicida; Yersinia pestis; Staphylococcus aureus; Mycobacterium avium subsp. paratuberculosis; Cowpox virus; Escherichia coli O157:H7; Francisella tularensis subsp. mediasiatica; Paslahepevirus balayani
Type:
Methylation profiling by array
Platform:
GPL21445
4 Samples
Download data: IDAT, TXT
Series
Accession:
GSE267014
ID:
200267014
3.

Cyclical transcription factor AP2XII-9 is a key activator for asexual division and apicoplast inheritance in Toxoplasma gondii tachyzoite

(Submitter supplied) Toxoplasma gondii is an intracellular parasitic protozoan that poses a significant risk to pregnant women and immunocompromised individuals. T. gondii tachyzoites duplicate rapidly in host cells during acute infection through endodyogeny. This highly regulated division process is accompanied by complex gene regulation networks. TgAP2XII-9 is a cyclical transcription factor, but its specific role in the parasite cell cycle is not fully understood. more...
Organism:
Toxoplasma gondii
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL26742
4 Samples
Download data: BW, NARROWPEAK
Series
Accession:
GSE266204
ID:
200266204
4.

Transcriptome analysis of cytotrophoblasts and syncytiotrophoblasts derived from trophoblast stem cells, mock-infected or infected with Toxoplasma gondii

(Submitter supplied) We tested the relevance of an established stem-cell-derived model of trophoblast development to Toxoplasma gondii infection. Human trophoblast stem cells were cultured under conditions suitable for cytotrophoblast cells or for the differentiation into syncytiotrophoblasts, and subsequently infected with T. gondii. RNA-seq data from both mock-treated and infected cells revealed differences between cell types and their respective responses to T. more...
Organism:
Toxoplasma gondii; Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL30173 GPL34372
12 Samples
Download data: TXT
Series
Accession:
GSE263615
ID:
200263615
5.

Translation initiation factor eIF1.2 orchestrates Toxoplasma gondii differentiation by regulating stage-specific gene expression

(Submitter supplied) Toxoplasma gondii persists in humans by converting from actively replicating acute stage tachyzoites to slow-growing chronic stage bradyzoites. The molecular mechanisms that mediate T. gondii differentiation remain poorly understood. Through a chemical mutagenesis screen, we identified translation initiation factor eIF1.2 as being critical for T. gondii differentiation. The presence of an F97L mutation in eIF1.2 identified in the screen or the complete lack eIF1.2 (∆eIF1.2) markedly impeded bradyzoite cyst formation in culture and in the brains of infected mice. more...
Organism:
Toxoplasma gondii
Type:
Expression profiling by high throughput sequencing; Other
Platform:
GPL23466
24 Samples
Download data: TXT
Series
Accession:
GSE245775
ID:
200245775
6.

Timecourse of transcriptional changes upon eIF4E1 depletion in Toxoplasma gondii

(Submitter supplied) The protozoan parasite Toxoplasma gondii causes serious opportunistic disease due to its ability to persist in patients as latent tissue cysts. The molecular mechanisms coordinating conversion between proliferative parasites (tachyzoites) and latent cysts (bradyzoites) are not fully understood. We previously showed that phosphorylation of eIF2α accompanies bradyzoite formation, suggesting that this clinically relevant process involves regulation of mRNA translation. more...
Organism:
Toxoplasma gondii
Type:
Expression profiling by high throughput sequencing
Platform:
GPL26742
18 Samples
Download data: TABULAR
Series
Accession:
GSE262241
ID:
200262241
7.

The transcription factor AP2XI-2 is a key negative regulator of Toxoplasma gondii merogony

(Submitter supplied) Commitment to and completion of sexual development are essential for Toxoplasma gondii to produce oocysts in the intestine of the feline host. Understanding of the molecular mechanism responsible for sexual commitment is extremely limited due to the lack of model systems. Here, we show that the transcription factors AP2XI-2 and AP2XII-1 associated with the epigenetic repressors MORC/HDAC3 complex are constitutively expressed in both tachyzoite and bradyzoite stages but not in the merozoite stage. more...
Organism:
Toxoplasma gondii
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL26742
6 Samples
Download data: BW
Series
Accession:
GSE248448
ID:
200248448
8.

Genome-wide binding of the bromdomain protein TgBDP3 in the protozoan parasite Toxoplasma gondii

(Submitter supplied) The bromdomain protein TgBDP3 is associated with the TFIID transcriptioanl initiation complex in Toxoplasma gondii. To determine the genes that are regulated by TgBDP3, the endogenous tgbdp3 locus was modified to introduce a C-terminal HA epitope tag. ChIP-seq was performed on intracellular, replicating tachyzoites expressing TgBDP3-HA. The protein was enriched in the upstream, promoter region of a subset of actively transcribed parasite genes indicating that TgBDP3 has a role in regulation of transcriptional initiation in Toxoplasma.
Organism:
Toxoplasma gondii
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL25973
5 Samples
Download data: BED
Series
Accession:
GSE124251
ID:
200124251
9.

Unveiling the Transcriptional Regulation of Development in Toxoplasma gondii: Insights into a Cascade AP2 Regulatory Network

(Submitter supplied) Toxoplasma gondii is a medically and veterinary important intracellular parasite that undergoes distinct developmental transitions in its intermediate and definitive hosts. The switch between stages of T. gondii is meticulously regulated by a variety of factors. Previous studies have explored the role of the microrchidia (MORC) protein complex as a transcriptional suppressor of sexual commitment. By utilizing immunoprecipitation and mass spectrometry, constituents of this protein complex have been identified, including MORC, Histone Deacetylase 3 (HDAC3), and several ApiApiAP2 transcription factors. more...
Organism:
Toxoplasma gondii
Type:
Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL26742
26 Samples
Download data: BW, TXT
Series
Accession:
GSE249604
ID:
200249604
10.

Depletion of mRNA cap-binding protein eIF4E1 drives bradyzoite formation in Toxoplasma gondii

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Toxoplasma gondii
Type:
Other; Expression profiling by high throughput sequencing
Platform:
GPL23466
24 Samples
Download data: BIGWIG, TXT
Series
Accession:
GSE243207
ID:
200243207
11.

Depletion of mRNA cap-binding protein eIF4E1 drives bradyzoite formation in Toxoplasma gondii [RIBO-seq]

(Submitter supplied) Ingestion of Toxoplasma gondii results in life-long infection due to its ability to convert from the rapidly disseminating tachyzoite stage to the chronic, encysted bradyzoite stage. The control of mRNA translation has been suggested to play a key role in the signaling required to trigger bradyzoite formation. In eukaryotes, translational control primarily operates at two key points during the assembly of translation initiation factors. more...
Organism:
Toxoplasma gondii
Type:
Other; Expression profiling by high throughput sequencing
Platform:
GPL23466
12 Samples
Download data: TXT
Series
Accession:
GSE243206
ID:
200243206
12.

Depletion of mRNA cap-binding protein eIF4E1 drives bradyzoite formation in Toxoplasma gondii [CLIP-seq]

(Submitter supplied) Ingestion of Toxoplasma gondii results in life-long infection due to its ability to convert from the rapidly disseminating tachyzoite stage to the chronic, encysted bradyzoite stage. The control of mRNA translation has been suggested to play a key role in the signaling required to trigger bradyzoite formation. In eukaryotes, translational control primarily operates at two key points during the assembly of translation initiation factors. more...
Organism:
Toxoplasma gondii
Type:
Other
Platform:
GPL23466
12 Samples
Download data: BED, BIGWIG
Series
Accession:
GSE243203
ID:
200243203
13.

In vitro production of cat-restricted Toxoplasma pre-sexual stages by epigenetic reprogramming

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Toxoplasma gondii
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platforms:
GPL23466 GPL26742
24 Samples
Download data: BW
Series
Accession:
GSE222835
ID:
200222835
14.

In vitro production of cat-restricted Toxoplasma pre-sexual stages by epigenetic reprogramming [ATAC-seq]

(Submitter supplied) Here we show that AP2XII-1 and AP2XI-2, two transcription factors expressed in tachyzoites, a stage that causes acute toxoplasmosis, silence genes specific to merozoites, a developmental stage critical for sexual commitment and transmission to the next host, including humans. Their conditional and simultaneous depletion leads to a drastic change in the transcriptional program, promoting a complete transition from tachyzoites to merozoites. more...
Organism:
Toxoplasma gondii
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL26742
4 Samples
Download data: BW, TXT
Series
Accession:
GSE222832
ID:
200222832
15.

In vitro production of cat-restricted Toxoplasma pre-sexual stages by epigenetic reprogramming [ChIP-seq]

(Submitter supplied) Here we show that AP2XII-1 and AP2XI-2, two transcription factors expressed in tachyzoites, a stage that causes acute toxoplasmosis, silence genes specific to merozoites, a developmental stage critical for sexual commitment and transmission to the next host, including humans. Their conditional and simultaneous depletion leads to a drastic change in the transcriptional program, promoting a complete transition from tachyzoites to merozoites. more...
Organism:
Toxoplasma gondii
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL23466
20 Samples
Download data: BW
Series
Accession:
GSE222819
ID:
200222819
16.

An ex vivo model of Toxoplasma recrudescence reveals the developmental plasticity of the bradyzoite stage

(Submitter supplied) Reactivation of toxoplasmosis poses a significant health risk to chronically infected people especially those with compromised immune systems. Molecular studies of reactivation have been difficult due to the lack of accurate models of bradyzoite recrudescence, which initiates disease reactivation. Here, we performed single cell RNA-sequencing (scRNA-seq) on parasite populations that form after bradyzoite infection of two host-cell types: primary mouse astrocytes and human foreskin fibroblasts. more...
Organism:
Toxoplasma gondii
Type:
Expression profiling by high throughput sequencing
Platform:
GPL26742
6 Samples
Download data: MTX, TSV
Series
Accession:
GSE210671
ID:
200210671
17.

Profound effect of a ubiquitin activating enzyme (UAE1) on genomic expression of Toxoplasma gondii

(Submitter supplied) Toxoplasma gondii is an intracellular parasite that relies on intricate molecular mechanisms for its proliferation and survival. Here, we investigate the role of TgUAE1 in these processes and shed light on the underlying regulatory network. We demonstrate that TgUAE1 is crucial for maintaining the proliferation of T. gondii and the normal morphology of mitochondria. To elucidate the molecular basis of TgUAE1's essential functions, we conducted transcriptome sequencing to identify the genes regulated by this factor. more...
Organism:
Toxoplasma gondii
Type:
Expression profiling by high throughput sequencing
Platform:
GPL26742
8 Samples
Download data: XLSX
Series
Accession:
GSE240648
ID:
200240648
18.

In vitro analysis of pH responsiveness of T. gondii and H. hammondi sporozoites, and of T. gondii tachyzoites lacking either the BFD1 or the ROCY1 gene.

(Submitter supplied) There are two experiments included in this particular GEO accession: For the first, freshly excysted sporozoites of T. gondii VEG or H. hammondi Amer1 were put into culture for 2 days, and then either allowed to continue growth in control (pH 7.2, 5% C02, 10% FCS) or high pH, bradyzoite-inducing (pH 8.2, low C02, 1% FCS) conditions for 3 days and then harvested for RNAseq analysis. In the second, T. more...
Organism:
Toxoplasma gondii; Hammondia hammondi
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL32894 GPL25973
27 Samples
Download data: TXT
Series
Accession:
GSE218921
ID:
200218921
19.

Brain transcriptome of mice infected with T. gondii parasites

(Submitter supplied) Mice were infected with up to 250,000 tachyzoites of T. gondii VEG strain parasites genetically engineered to be deficient in either the ROCY1 gene (TGVEG_311100) or the BFD1 gene (TGVEG_200385). Mice were infected intraperitoneally and monitored using in vivo bioluminescence imaging. Mice were sacrificed at either 4 weeks or 9 weeks post infection as indicated, and RNA was isolated from their brains and transcript abundance was determined using RNAseq.
Organism:
Toxoplasma gondii; Mus musculus
Type:
Expression profiling by high throughput sequencing
Platform:
GPL32893
19 Samples
Download data: XLSX
Series
Accession:
GSE218920
ID:
200218920
20.

ROCY1 binding sites in Toxoplasma gondii

(Submitter supplied) Cyst formation is a key feature of the T. gondii life cycle but the genetic networks that drive this process are not yet fully characterized. To identify new components of this network, we compared T. gondii to its nearest extant relative Hammondia hammondi given the critical differences between these species in the timing and efficiency of cyst formation. Using transcriptional data from critical developmental and pH exposure time points from both species, we identified the gene TGVEG_311100, which we named Regulator of Cystogenesis 1 (ROCY1), as being both necessary and sufficient for cyst formation in T. more...
Organism:
Toxoplasma gondii
Type:
Other
Platform:
GPL23377
6 Samples
Download data: BED, BIGWIG
Series
Accession:
GSE211957
ID:
200211957
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