txid5833[Organism:exp] - GEO DataSets

Search results

Items: 1 to 20 of 13797

<< First< Prev

Page of 690

Select item 2001890341.

Global transcriptional changes of Δpfdozi parasite in different stages and conditions

(Submitter supplied) Purpose: In malaria parasites, the regulation of mRNA translation, storage and degradation is a critical aspect of gene expression, especially during the life stage transition. Since the DEAD-box helicases are involved in RNA metabolism, we wanted to determine whether pfdozi disruption led to altered mRNA abundance in P. falciparum. Methods: In this study, we functionally characterized the DEAD-box RNA helicase PfDOZI in the human malaria parasite Plasmodium falciparum and discovered its functions on mRNA metabolism during development. more...

Organism:: Plasmodium falciparum

Type:: Expression profiling by high throughput sequencing

Platforms:: GPL21078 GPL19269
44 Samples

Download data: FASTA, XLSX

Series

Accession:: GSE189034

ID:: 200189034

SRA Run Selector

Select item 2002280972.

Knockdown of Plasmodium falciparum Jumonji C2 histone demethylase leads to a global perturbance of gene expression but does not alter var gene transcription

(Submitter supplied) The gene expression of the human malaria parasite Plasmodium falciparum is dynamically controlled by multiple factors and events including chromatin modifiers. Here, we addressed the effect of a temporary knockdown of the non-essential putative chromatin modifier P. falciparum JumonjiC2 histone demethylase (JmjC2) during asexual blood stages. While ribozyme-mediated transcript-knockdown of PfJmjC2 resulted in the differential transcription of many genes culminating in a delay of cycle progression, ChIPseq analysis pointed to only a few loci with which JmjC2 seemed to associate, including variant gene encoding loci. more...

Organism:: Plasmodium falciparum

Type:: Expression profiling by high throughput sequencing

Platform:: GPL21298
6 Samples

Download data: CSV

Series

Accession:: GSE228097

ID:: 200228097

Select item 2002455963.

Expression analysis of Plasmodium falciparum with MYST overexpression

(Submitter supplied) Investigation of genome-wide gene expression changes in Plasmodium falciparum overexpressing PfMYST compared to the wild-type strain 3D7. After introducing a single copy of the full-length PfMYST expression cassette into the parasite genome, parasites showed higher levels of H4-K5, -K8, and -K12 acetylation, a faster progression of intraerythrocytic developmental cycle (IDC), and shorter schizont development time (duration), which led to significantly fewer merozoites developed in mature schizonts than the control. more...

Organism:: Plasmodium falciparum; Plasmodium falciparum 3D7

Type:: Expression profiling by array

Platform:: GPL15768
12 Samples

Download data: PAIR, TXT

Series

Accession:: GSE245596

ID:: 200245596

PubMed Similar studies

Select item 2002406434.

Distinct transcriptomic signatures define febrile malaria depending on initial infective states, asymptomatic or uninfected

(Submitter supplied) Cumulative malaria parasite exposure in endemic regions often results in the acquisition of partial immunity and asymptomatic infections. There is limited information on how host-parasite interactions mediate maintenance of chronic symptomless infections that sustain malaria transmission. Here, we have determined the gene expression profiles of the parasite population and the corresponding host peripheral blood mononuclear cells (PBMCs) from 21 children (<15 years). more...

Organism:: Plasmodium falciparum 3D7

Type:: Expression profiling by high throughput sequencing

Platform:: GPL28887
32 Samples

Download data: TXT

Series

Accession:: GSE240643

ID:: 200240643

PubMed Full text in PMC Similar studies

Select item 2002351425.

Effects of the G-quadruplex-binding drugs Quarfloxin and CX-5461 on the malaria parasite Plasmodium falciparum

(Submitter supplied) P. falciparum is the deadliest causative agent of human malaria. This parasite has historically developed resistance to many drugs, including the current frontline treatments, so new therapeutic targets are needed Our previous work on guanine quadruplexes (G4s) in the parasite’s DNA and RNA has highlighted their influence on parasite biology, and also revealed G4 stabilising compounds as promising candidates for drug repositioning. more...

Organism:: Plasmodium falciparum

Type:: Expression profiling by high throughput sequencing

Platform:: GPL21298
9 Samples

Download data: TXT

Series

Accession:: GSE235142

ID:: 200235142

PubMed Full text in PMC Similar studies

Select item 2002278736.

P. falciparum-specific Genomic Context Protein-Binding Microarray

(Submitter supplied) Development of the human malaria parasite, Plasmodium falciparum is regulated by a limited number of sequence-specific transcription factors (TFs). However, the mechanisms by which these TFs recognize genome-wide binding sites to regulate target genes is still largely unknown. To address TF target specificity, we investigated the binding of two TF subsets that either bind CACACA or GTGCAC and further characterized PfAP2-G and PfAP2-EXP which bind unique DNA motifs (GTAC and TGCATGCA). more...

Organism:: Plasmodium falciparum

Type:: Other

Platform:: GPL33273
12 Samples

Download data: GPR, TXT

Series

Accession:: GSE227873

ID:: 200227873

Select item 2002413137.

Plasmodium falciparum MORC protein modulates gene expression through interaction with heterochromatin

(Submitter supplied) The MORC (microrchidia) family of proteins is highly conserved in all eukaryotic cells and have been shown to play diverse roles by forming protein-protein interactions with immune-responsive proteins, SWI chromatin remodeling complexes, histone deacetylases, and histone tail modifications across metazoans. To dissect the functional roles of MORC in the human malaria parasite, Plasmodium falciparum, we developed a glmS based ribozyme knockdown system to induce PfMORC knockdown upon glucosamine (GlcN) induction. more...

Organism:: Plasmodium falciparum

Type:: Expression profiling by high throughput sequencing

Platform:: GPL33701
12 Samples

Download data: SF, TXT

Series

Accession:: GSE241313

ID:: 200241313

Select item 2002412778.

RNAseq analysis of Plasmodium falciparum extracellular vesicles

(Submitter supplied) Plasmodium falciparum secretes extracellular vesicles that contain RNA. The biological benefit of this secretion to the secreting parasite is not known. Here, we sequenced the RNA content of extracellular vesicles and compared with that of the secreting whole parasites. The data suggests that extracellular vesicles might be part of a post-transcriptional regulatory mechanism that shapes intracellular RNA levels in the parasite.

Organism:: Plasmodium falciparum

Type:: Expression profiling by high throughput sequencing

Platform:: GPL27825
140 Samples

Download data: XLSX

Series

Accession:: GSE241277

ID:: 200241277

PubMed Full text in PMC Similar studies

Select item 2002302069.

PfAP2-P DNA-binding protein is a master regulator of parasite pathogenesis during malaria parasite blood stages (ChIP-Seq II)

(Submitter supplied) Malarial parasite pathogenicity results from its ability to invade and remodel red blood cells (RBCs), expressing antigenic variant proteins for immune evasion and survival, and then to egress from the host cell. These sequential processes require concerted actions of a large number of proteins during the intraerythrocytic developmental cycle (IDC), but the molecular basis of the required regulation is only partially understood. more...

Organism:: Plasmodium falciparum

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL26836
32 Samples

Download data: BED, BW

Series

Accession:: GSE230206

ID:: 200230206

PubMed Full text in PMC Similar studies

Select item 20019102510.

PfAP2-P DNA-binding protein is a master regulator of parasite pathogenesis during malaria parasite blood stages (scRNA-seq)

Organism:: Plasmodium falciparum

Type:: Expression profiling by high throughput sequencing

Platform:: GPL26836
4 Samples

Download data: TAR

Series

Accession:: GSE191025

ID:: 200191025

PubMed Full text in PMC Similar studies

Select item 20019051911.

PfAP2-P DNA-binding protein is a master regulator of parasite pathogenesis during malaria parasite blood stages

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Plasmodium falciparum

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:: GPL26836 GPL26835 GPL27825
59 Samples

Download data: BED, BW, TAR

Series

Accession:: GSE190519

ID:: 200190519

PubMed Full text in PMC Similar studies

Select item 20019049712.

PfAP2-P DNA-binding protein is a master regulator of parasite pathogenesis during malaria parasite blood stages (ChIP-Seq I)

Organism:: Plasmodium falciparum

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL26835
11 Samples

Download data: BED, BW

Series

Accession:: GSE190497

ID:: 200190497

PubMed Full text in PMC Similar studies

Select item 20019034213.

PfAP2-P DNA-binding protein is a master regulator of parasite pathogenesis during malaria parasite blood stages (RNA-seq)

Organism:: Plasmodium falciparum

Type:: Expression profiling by high throughput sequencing

Platform:: GPL27825
12 Samples

Download data: TXT

Series

Accession:: GSE190342

ID:: 200190342

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20022407314.

Genome-wide binding sites of MCM6 in Plasmodium falciparum

(Submitter supplied) Genome-wide ChIP-sequencing analysis of PfMCM6 was carried out in trophozoite stage parasites using PfMCM6 antibodies. We have observed that PfMCM6 is highly enriched at the exon regions. Moreover, PfMCM6 was also found in promoter-TSS, transcription termination site (TTS), and intergenic regions in minimal proportion. This study shed some light on PfMCM6 binding sites in Plasmodium falciparum genome.

Organism:: Plasmodium falciparum

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL21078
2 Samples

Download data: BROADPEAK

Series

Accession:: GSE224073

ID:: 200224073

PubMed Similar studies

Select item 20023373915.

Transcriptional profiling of primary human erythroblasts infected with Plasmodium falciparum

(Submitter supplied) In malaria infection, Plasmodium spp. parasites accumulate in the bone marrow near sites of erythroid development. While it has been observed that Plasmodium falciparum infection of late-stage erythroblasts can delay terminal erythroid differentiation and enucleation, the mechanism(s) underlying this phenomenon are unknown. Here, we apply RNA-seq after fluorescence-activated cell sorting (FACS) of infected erythroblasts to identify transcriptional responses to direct and indirect interaction with P. more...

Organism:: Plasmodium falciparum; Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platforms:: GPL24676 GPL33445
83 Samples

Download data: TXT

Series

Accession:: GSE233739

ID:: 200233739

PubMed Full text in PMC Similar studies

Select item 20019791616.

Metabolic competition between lipid metabolism and histone methylation regulates sexual differentiation in human malaria parasites

(Submitter supplied) Like most pathogens, malaria parasites balance persistence in the current host with transmission to the next. For Plasmodium falciparum, the most widespread and virulent human malaria parasite, persistence depends on continuous asexual replication in red blood cells while transmission requires differentiation into non-replicating gametocytes, the male and female cells able to infect the mosquito vector. more...

Organism:: Plasmodium falciparum

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL32020
30 Samples

Download data: BED, BEDGRAPH

Series

Accession:: GSE197916

ID:: 200197916

PubMed Similar studies

Select item 20021453517.

Chromatin profiling of H3R2me2s in human malaria parasite

(Submitter supplied) Purpose: this study is to analyze the chromatin landscape of H3R2mes in Plasmodium falciparum. Methods: In this study, H3R2me2s enrichment in the chromatin of wildtype parasite at schizont stage was analyzed by Cleavage Under Targets and Tagmentation (CUT&Tag) coupled with next generation sequencing (CUT&Tag-seq). Synchronized WT 3D7 at 40–46 hpi schizonts were harvested and fixed with 1% formaldehyde for 1 min at room temperature, followed by lysis with 0.06% saponin. more...

Organism:: Plasmodium falciparum

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL26920
6 Samples

Download data: XLSX

Series

Accession:: GSE214535

ID:: 200214535

PubMed Full text in PMC Similar studies

Select item 20019941918.

Transcriptional alteration in malaria parasite after disruption of PfPRMT5 [Array]

(Submitter supplied) Purpose: this study is to analyze the change of overall transctiption after disruption of Protein Arginine Methyltranferase 5 (PRMT5) in Plasmodium falciparum. Methods: In this study, the transcriptomes of a PfPRMT5 gene knockout (KO) parasite line with its wildtype control were analyzed a custom-desinged Agilent microarray.Total RNA were harvested from the asexual parasites at four stages (ring, early trophozoite, late trophozoite, and schizont)(12h, 24h, 36h, and 46h post-invasion) using the Quick-RNA MiniPrep kit (Zymo Research).

Organism:: Plasmodium falciparum

Type:: Expression profiling by array

Platform:: GPL21379
24 Samples

Download data: TXT, XLSX

Series

Accession:: GSE199419

ID:: 200199419

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20019936619.

Transcriptome of ΔPfPRMT5 parasite during malaria parasite asexual development [PRMT5_RNAseq]

(Submitter supplied) Purpose: this study is to analyze the change of RNA splicing events after disruption of Protein Arginine Methyltranferase 5 (PRMT5) in Plasmodium falciparum. Methods: In this study, the transcriptomes of a PfPRMT5 gene knockout (KO) parasite line with its wildtype control were analyzed by RNAseq.Total RNA were harvested from the asexual parasites at four stages (ring, early trophozoite, late trophozoite, and schizont)(12h, 24h, 36h, and 46h post-invasion) using the Quick-RNA MiniPrep kit (Zymo Research). more...