GEO DataSets

(Submitter supplied) The Baculovirus Expression Vector System (BEVS) is highly valued in vaccine development, protein engineering, and drug metabolism research due to its biosafety, operational convenience, rapid scalability, and capacity for self-assembling virus-like particles. However, increasing cell density at the time of inoculation severely compromises the production capacity of BEVS, resulting in the “cell density effect”. more...

Organism:

Trichoplusia ni

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17310

36 Samples

Download data: TXT

(Submitter supplied) Cruciferous plants produce sulforaphane (SFN), an inhibitor of nuclear histone deacetylases (HDACs). In humans and other mammals, the consumption of SFN alters enzyme activities, DNA-histone binding, and gene expression within minutes. However, the ability of SFN to act as a HDAC inhibitor in nature, disrupting the epigenetic machinery of insects feeding on these plants, has not been explored. Here, we investigate the effect of SFN, as well as the pharmaceutical HDAC inhibitor Trichostatin A (TSA), consumed in the diet in the generalist grazer Spodoptera exigua and in the co-evolved specialist feeder Trichoplusia ni. more...

Organism:

Spodoptera exigua; Trichoplusia ni

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL33468 GPL29211

20 Samples

Download data: TXT

(Submitter supplied) We used RNA-Sequencing technology to analyze the transcriptomes of two ascovirus isolates, SfAV and TnAV, over three weeks.

Organism:

Spodoptera frugiperda; Trichoplusia ni

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL27985 GPL30114

48 Samples

Download data: XLSX

(Submitter supplied) Purpose: To compare the TnAV-6a1 variant infected Trichoplusia ni larval transcriptome of hemolymph viral vesicles versus those of other major larval tissues specifically, the fat body, epidermis and tracheal matrix (referred to as FET tissues).

Organism:

Trichoplusia ni

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23793

26 Samples

Download data: TXT, XLSX

(Submitter supplied) Despite the essentiality for faithful chromosome segregation, centromere architectures are diverse among eukaryotes and embody two main configurations: mono- and holocentromeres, referring respectively to localized or unrestricted distribution of centromeric activity. Of the two, some holocentromeres offer the curious condition of having arisen independently in multiple insects, most of which have lost the otherwise essential centromere-specifying factor CenH3 (first described as CENP-A in humans). more...

Organism:

Bombyx mori; Trichoplusia ni

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

5 related Platforms

41 Samples

Download data: BW

(Submitter supplied) We report on the transcriptome differences in the salivary glands of cabbage looper larvae fed on different diets

Organism:

Trichoplusia ni

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23793

9 Samples

Download data: TXT

(Submitter supplied) We solved the crystal structure of human ARGONAUTE1 (hAGO1) bound to endogenous 5'-phosphorylated guide RNAs. To identify structural changes that evolutionarily rendered hAGO1 inactive, we compared our structure with published structures of guide-RNA-containing and cleavage-active hAGO2. Aside from mutation of a catalytic tetrad residue, proline residues at positions 670 and 675 in hAGO1 introduce a kink in the cS7 loop and thereby forming a convex surface within the hAGO1 nucleic-acid-binding channel near the inactive catalytic site. more...

Organism:

Trichoplusia ni

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL17310

4 Samples

Download data: TXT