GEO DataSets

(Submitter supplied) This study describes the application of the LongSAGE methodology to study the gene expression profile in Leishmania infantum chagasi. A tag library was created using the LongSAGE method and consisted of 14,208 tags of 17 bases. Of these, 8,427 (59.3%) were distinct. BLAST research of the 1,645 most abundant tags showed that 12.8% of them identified the coding sequences of genes, while 82% (1,349/1,645) identified one or more genomic sequences that did not correspond with open reading frames. more...

Organism:

Leishmania chagasi

Type:

Expression profiling by SAGE

Platform:

GPL11414

1 Sample

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(Submitter supplied) Transcriptional analyses of L. infantum promastigote compared to L. infantum intracellular amastigote, and L. major promastigote compared to L. major intracellular amastigote The full-genome DNA microarray includes one 70mer-oligonucleotide probe for each gene of L. infantum and for each gene of L.major LV39 Keywords: stage-specific comparison

Organism:

Leishmania major; Leishmania infantum

Type:

Expression profiling by array

Platform:

GPL6855

10 Samples

Download data: GPR

(Submitter supplied) Leishmania infantum (Kinetoplastida:Trypanosomatidae) is the etiological agent of zoonotic visceral leishmaniasis in the Mediterranean basin. The motile promastigote stage infects the hematophagous sand fly vector host and amastigotes survives and multiplies within phagocytes of the mammalian host. Promastigotes are routinely cultured in liquid undefined media and are considered to mimic the environment within the sand fly gut. more...

Organism:

Leishmania infantum

Type:

Expression profiling by array

Platform:

GPL6781

1 Sample

Download data: TXT

(Submitter supplied) Leishmania infantum is the causative agent of zoonotic visceral leishmaniasis in Mediterranean areas and also acts as an opportunistic parasite in HIV patients. Metacyclic promastigotes are transmitted during bloodmeals of the sand-fly host after development. Metacyclogenesis can be micmiked in axenic cultures and peanut lectin (PNA) agglutination followed by two-step centrifugation allows the separation of procyclic and metacyclic promastigotes in L. more...

Organism:

Leishmania infantum

Type:

Expression profiling by array

Platform:

GPL6781

3 Samples

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(Submitter supplied) Transcriptional analysis of L. infantum promastigote compared to L. infantum intracellular amastigote and transcriptional analysis of L. infantum promastigote compared to L. infantum axenic amastigote. The full-genome DNA microarrays includes one 70-oligonucleotides probe for each gene of L.infantum. Keywords: stage and culture condition

Organism:

Leishmania major; Leishmania infantum

Type:

Expression profiling by array

Platform:

GPL6855

10 Samples

Download data: GPR

(Submitter supplied) A laboratory colony of Phlebotomus perniciosus sand flies was maintained. Sand flies were infected with cultured Leishmania infantum promastigotes in stationary phase. Ten infected sand flies were dissected after 5 days and promastigotes within the gut pooled. The cells were immediately washed in PBS once and lysed in TRIzol reagent (Life Technologies). RNA isolation was completed according to the manufacturer's instructions, obtaining 63ng. more...

Organism:

Leishmania infantum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23911

2 Samples

Download data: XLSX

(Submitter supplied) In this study, we examined the transcriptome of Leishmania donovani promastigotes and axenic amastigotes to identify differentially regulated mRNAs utilizing the serial analysis of gene expression Keywords: stage differentiation; axenic amastigotes

Organism:

Leishmania donovani

Type:

Expression profiling by SAGE

Platform:

GPL3252

2 Samples

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(Submitter supplied) APOE4 allele is a major risk factor for late-onset Alzheimer disease (AD). The mechanism of action of APOE in AD remains unclear. To study the effects of APOE alleles on gene expression in AD, we have analyzed the gene transcription patterns of human hippocampus from APOE3/3, APOE3/4, APOE4/4 AD patients and normal control using Serial Analysis of Gene Expression (SAGE). Using SAGE, we found gene expression patterns in hippocampus of APOE3/4 and APOE4/4 AD patients differ substantially from those of APOE3/3 AD patients. more...

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platforms:

GPL4 GPL1485

6 Samples

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(Submitter supplied) CGH analysis of the drug resistant strain L. major MTX60.4 vs the wild-type strain LV39 The full-genome DNA microarrays includes one 70-oligonucleotides probe for each gene of L.major LV39 Keywords: drug resistance

Organism:

Leishmania major; Leishmania infantum

Type:

Genome variation profiling by array

Platform:

GPL6855

3 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Leishmania infantum; Leishmania major

Type:

Expression profiling by array

Platform:

GPL6855

8 Samples

Download data: GPR

(Submitter supplied) Transcriptional analysis of the drug resistant strain L. major MTX60.4 compared to the wild-type strain LV39 The full-genome DNA microarrays includes one 70-oligonucleotides probe for each gene of L.major LV39 Keywords: drug resistance

Organism:

Leishmania infantum; Leishmania major

Type:

Expression profiling by array

Platform:

GPL6855

4 Samples

Download data: GPR

(Submitter supplied) Transcriptional analysis of the drug resistant strain L. infantum MTX20.5 compared to the wild-type strain The full-genome DNA microarrays includes one 70-oligonucleotides probe for each gene of L. infantum Keywords: drug resistance

Organism:

Leishmania infantum; Leishmania major

Type:

Expression profiling by array

Platform:

GPL6855

4 Samples

Download data: GPR

(Submitter supplied) Owing to its increased tag length, LongSAGE tags are expected to be more reliable in direct assignment to genome sequences. Therefore, we evaluated the use of LongSAGE data in genome annotation by using our LongSAGE dataset of 202 015 tags (consisting of 41 718 unique tags), experimentally generated from mouse embryonic tail libraries. RESULTS: A fraction of LongSAGE tags could not be unambiguously assigned to its gene, due to the presence of widely conserved sequences downstream of particular CATG anchor sites. more...

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL1349

1 Sample

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(Submitter supplied) A genome wide microarray platform containing the predicted coding sequences (putative genes) for the ciliated protozoan Tetrahymena thermophila is described, validated and used to study gene expression in growing, starved and conjugating cells. Of the ~27,400 predicted open reading frames in Tetrahymena, transcripts homologous to 5876 are not detectable in one or more of these life cycle stages, indicating that this organism does indeed contain a large number of functional genes. more...

Organism:

Tetrahymena thermophila

Type:

Expression profiling by array

Platform:

GPL6759

50 Samples

Download data: TXT

(Submitter supplied) Leishmania (L) are intracellular protozoan parasites which are able to survive and replicate within the harsh and potentially hostile phago-lysosomal environment of mammalian mononuclear phagocytes. A complex interplay then takes place between the macrophage (MΦ) striving to eliminate the pathogen and the parasite struggling for its own survival. To investigate, at the transcriptional level, this host-parasite conflict in the context of monocyte-derived human MΦs (MDM) infection by L. more...

Organism:

Leishmania major; Homo sapiens

Type:

Expression profiling by SAGE

Platforms:

GPL6471 GPL4

3 Samples

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(Submitter supplied) Using the RL-SAGE method (Gowda et al. 2004), a maize leaf longSAGE library (cv. inbred line B73) was constructed. Leaf tissues were harvested from 4-week old B73 plants for RNA isolation. The conditions in the growth chamber were 12 h light (500 µmol photons m-2 sec-1), 20oC at night, 26oC in the day and 85% relative humidity. A total of 44,870 unique tags (17 bases +CATG) were identified from 232,948 individual tags in the maize leaf library.

Organism:

Zea mays

Type:

Expression profiling by SAGE

Platform:

GPL1462

1 Sample

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