GEO DataSets

(Submitter supplied) We assessed changes in gene expression in response to zinc availability for the human pathogen Corynebacterium diphtheriae. Expression profiles of wild-type C. diphtheriae strain 1737 grown in semi-defined metal-poor media (mPGT) without and with 5 µM zinc chloride supplementation were compared; the expression profile of wild-type C. diphtheriae strain 1737 in zinc-replete conditions was also compared against an isogenic Δzur mutant grown in zinc-replete conditions. more...

Organism:

Corynebacterium diphtheriae NCTC 13129; Corynebacterium diphtheriae

Type:

Expression profiling by array

Platform:

GPL25539

9 Samples

Download data: TXT, XLSX

(Submitter supplied) We assessed changes in gene expression in response to manganese availability for the human pathogen Corynebacterium diphtheriae. Total RNA was harvested from wild-type C. diphtheriae strain 1737 and an isogenic ΔmntR (dip0619) grown in semi-defined metal-limited media (mPGT) without or with 5 µM manganese chloride supplementation. Three biological replicates were prepared and five genes were assessed by real-time PCR to validate the array results: dip0124, dip0169, dip0615, dip1923, and dip2261. more...

Organism:

Corynebacterium diphtheriae

Type:

Expression profiling by array

Platform:

GPL30087

12 Samples

Download data: TXT

(Submitter supplied) Background: The human pathogen Corynebacterium diphtheriae is the causative agent of the disease diphtheria. In the 1990s a large diphtheria outbreak in Eastern Europe was caused by the strain C. diphtheriae NCTC 13129. Although the genome was sequenced several years ago, not much is known about the transcriptome. Our aim was to use RNA Sequencing to close this knowledge gap and gain insights into the transcriptional landscape of C. more...

Organism:

Corynebacterium diphtheriae NCTC 13129

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23376

7 Samples

Download data: XLSX

(Submitter supplied) Intracellular zinc concentration needs to be maintained within strict limits due to its toxicity at high levels, and this is achieved by a finely regulated balance between uptake and efflux. Many bacteria use the Zinc Uptake Regulator Zur to orchestrate zinc homeostasis, but little is known regarding the transport of this metal across the bacterial outer membrane. In this work we determined the C. crescentus Zur regulon by global transcriptional and in silico analyses. more...

Organism:

Caulobacter vibrioides CB15; Caulobacter vibrioides NA1000

Type:

Expression profiling by array

Platform:

GPL10469

3 Samples

Download data: TXT

(Submitter supplied) We used DNA microarrays to define the M. tuberculosis FurB regulon. For this porpouse we constructed a M. tuberculosis strain where furB was disrupted and its transcriptional profile was compared on DNA microarray with the transcriptional profile of its parental strain (H37Rv). A total of 32 genes included in 16 putative transcription units were shown to be up-regulated in the mutant relative to H37Rv. more...

Organism:

Mycobacterium tuberculosis

Type:

Expression profiling by array

Platform:

GPL4057

6 Samples

Download data

(Submitter supplied) The zur regulon in Neisseria meningitidis was elucidated in the strain MC58 using a zur knockout strain and conditions which activate Zur ( zinc supplementation in the medium)

Organism:

Neisseria meningitidis

Type:

Expression profiling by array

Platform:

GPL8787

11 Samples

Download data: GPR, TXT

(Submitter supplied) We determined the genomic locations of the Zur binding sites by chromatin immuno-precipitation of cross-linked DNA and hybridization on a tilling oligonucleotide array on exponentially grown cultures of M145 and zur mutant.

Organism:

Streptomyces coelicolor; Streptomyces coelicolor A3(2)

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL14825

6 Samples

Download data: GFF, PAIR

(Submitter supplied) Investigation of whole genome gene expression level changes in a Clostridium difficile fur (ferric uptake regulator) mutant, compared to the wild type strain 630 erm. The fur mutant analyzed in this study is further described in Ho and Ellermeier (2015) J. Bacteriology

Organism:

Clostridioides difficile 630

Type:

Expression profiling by array

Platform:

GPL20243

12 Samples

Download data: PAIR

(Submitter supplied) The transcriptional factor Zur plays a key role in regulating zinc homeostasis in Bacillus subtilis. The genomic sites bound by Zur were mapped using a chromatine immunoprecipitation approach. This allowed the identification of 80 inter- and intragenic chromosomal sites bound by Zur.

Organism:

Bacillus subtilis subsp. subtilis str. 168

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL13168

2 Samples

Download data: PAIR

(Submitter supplied) In this study wild-type, fur mutant, and complemented fur mutant strains of the human pathogen Neisseria gonorrhoeae F62 were grown under high (100 uM iron) or low (100 uM desferal) iron conditions to identify genes whose expression was regulated by iron and/or Fur

Organism:

Neisseria gonorrhoeae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20721

10 Samples

Download data: TXT

(Submitter supplied) Response of Cupriavidus metallidurans AE104 and delta zur mutant to Zn stress

Organism:

Cupriavidus metallidurans CH34

Type:

Expression profiling by array

Platform:

GPL16115

9 Samples

Download data: TXT

(Submitter supplied) RNA-seq differential gene expression profiling of Corynebacterium pseudotuberculosis under iron limitation. We use two C. pseudotuberculosis strains, a ciuA Cp13 mutant and its parental wild type T1 strain to quantify the relative gene expression of these strains in cultures with low iron availability. The ciuA Cp13 mutant is deficient for an operon which encodes a iron siderophore uptake system. The Cp13 mutant also showed reduced virulence in CLA infection models. more...

Organism:

Corynebacterium pseudotuberculosis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24971

14 Samples

Download data: TXT

(Submitter supplied) RNA degradation is a crucial process in bacterial cells for maintaining proper transcriptome homeostasis and coping with changing environments. A specialized ribonuclease known as RNase J (RnJ) participates in mRNA turnover in many Gram-positive bacteria; however, nothing is known about this process in Corynebacterium diphtheriae, nor is the identity of this RNase. We report here that C. diphtheriae DIP1463 encodes a predicted RnJ homolog, comprised of an N-terminal beta-lactamase domain, followed by beta-CASP and C-terminal domains. more...

Organism:

Corynebacterium diphtheriae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29654

6 Samples

Download data: XLSX