ADAM28 ADAM metallopeptidase domain 28 [Homo sapiens (human)] - Gene

Summary

Official Symbol: ADAM28provided by HGNC
Official Full Name: ADAM metallopeptidase domain 28provided by HGNC
Primary source: HGNC:HGNC:206
See related: Ensembl:ENSG00000042980 MIM:606188; AllianceGenome:HGNC:206
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: MDCL; MDC-L; eMDCII; ADAM 28; eMDC II
Summary: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is a lymphocyte-expressed ADAM protein. This gene is present in a gene cluster with other members of the ADAM family on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
Expression: Biased expression in stomach (RPKM 21.4), gall bladder (RPKM 11.1) and 12 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 8p21.2

Exon count:: 29

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	8	NC_000008.11 (24294069..24359014)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	8	NC_060932.1 (24569171..24634154)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	8	NC_000008.10 (24151582..24216527)

Chromosome 8 - NC_000008.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

LncRNA NEAT1 induces autophagy through the miR-128-3p/ADAM28 axis to suppress apoptosis of nonsmall-cell lung cancer.
Title: LncRNA NEAT1 induces autophagy through the miR-128-3p/ADAM28 axis to suppress apoptosis of nonsmall-cell lung cancer.
ADAM28 from both endothelium and gastric cancer cleaves von Willebrand Factor to eliminate von Willebrand Factor-induced apoptosis of gastric cancer cells.
Title: ADAM28 from both endothelium and gastric cancer cleaves von Willebrand Factor to eliminate von Willebrand Factor-induced apoptosis of gastric cancer cells.
ADAM28: Another ambivalent protease in cancer.
Title: ADAM28: Another ambivalent protease in cancer.
[Construction of ADAM28 shRNA interference vector and its inhibitory effect on human periodontal ligament stem cells].
Title: [Construction of ADAM28 shRNA interference vector and its inhibitory effect on human periodontal ligament stem cells].
Study demonstrates that ADAM28 is overexpressed in pancreatic cancer, and closely involved in the regulation of gemcitabine resistance. Overexpression of ADAM28 is a novel prognostic biomarker in pancreatic cancer.
Title: Oncogenic ADAM28 induces gemcitabine resistance and predicts a poor prognosis in pancreatic cancer.
ADAM28 regulates the growth and dissemination of acute myeloid leukemia through IGFBP-3 degradation and IGF-I-induced cell proliferation.
Title: ADAM28 promotes tumor growth and dissemination of acute myeloid leukemia through IGFBP-3 degradation and IGF-I-induced cell proliferation.
Within placental villi, ADAM28 mRNA levels were highest in HLA-G(+) column trophoblasts, and consistent with this, ADAM28 was preferentially localized to HLA-G(+) trophoblasts within distal anchoring columns and decidual tissue.
Title: ADAM28 localizes to HLA-G(+) trophoblasts and promotes column cell outgrowth.
There is a relationship between the serum level of ADAM28 and the degree of the colonic cancer clinical stage.
Title: Serum levels of ADAM10, ADAM12, ADAM17 AND ADAM28 in colorectal cancer patients.
miR-552 is an oncomir able to promote CRC metastasis in part through a mechanism of targeting ADAM28.
Title: MicroRNA-552 enhances metastatic capacity of colorectal cancer cells by targeting a disintegrin and metalloprotease 28.
The data demonstrate that ADAM28 is expressed by epithelial cells of several normal organs, and suggest that ADAM28 plays a role in cell survival by suppression of C1q-induced cytotoxicity in bronchial epithelial cells.
Title: ADAM28 is expressed by epithelial cells in human normal tissues and protects from C1q-induced cell death.

Submit: New GeneRIF Correction See all GeneRIFs (34)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Hundreds of variants clustered in genomic loci and biological pathways affect human height. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables immunoglobulin receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables metalloendopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables metallopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in proteolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in spermatogenesis	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	IDA Inferred from Direct Assay more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info

General protein information

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Preferred Names: disintegrin and metalloproteinase domain-containing protein 28

Names: epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II; epididymial metalloproteinase-like, disintegrin-like, and cysteine-rich protein II; epididymis secretory sperm binding protein; metalloproteinase-like, disintegrin-like, and cysteine-rich protein-L

NP_001291280.1

EC 3.4.24.-

NP_055080.2

EC 3.4.24.-

NP_068547.2

EC 3.4.24.-

XP_005273437.1

EC 3.4.24.-

XP_005273439.1

EC 3.4.24.-

XP_006716336.1

EC 3.4.24.-

XP_006716337.1

EC 3.4.24.-

XP_011542669.1

EC 3.4.24.-

XP_011542670.1

EC 3.4.24.-

XP_011542671.1

EC 3.4.24.-

XP_011542673.1

EC 3.4.24.-

XP_016868463.1

EC 3.4.24.-

XP_016868464.1

EC 3.4.24.-

XP_047277226.1

EC 3.4.24.-

XP_047277227.1

EC 3.4.24.-

XP_047277228.1

EC 3.4.24.-

XP_047277229.1

EC 3.4.24.-

XP_047277230.1

EC 3.4.24.-

XP_054215572.1

EC 3.4.24.-

XP_054215573.1

EC 3.4.24.-

XP_054215574.1

EC 3.4.24.-

XP_054215575.1

EC 3.4.24.-

XP_054215576.1

EC 3.4.24.-

XP_054215577.1

EC 3.4.24.-

XP_054215578.1

EC 3.4.24.-

XP_054215579.1

EC 3.4.24.-

XP_054215580.1

EC 3.4.24.-

XP_054215581.1

EC 3.4.24.-

XP_054215582.1

EC 3.4.24.-

XP_054215583.1

EC 3.4.24.-

XP_054215584.1

EC 3.4.24.-

XP_054215585.1

EC 3.4.24.-

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_029394.2 RefSeqGene

Range

5003..69948

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001304351.2 → NP_001291280.1 disintegrin and metalloproteinase domain-containing protein 28 isoform 4 preproprotein

Status: REVIEWED

Description

Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. It encodes isoform 4 which is shorter than isoform 1.

Source sequence(s)

AC120193, AJ242015, AK225921, BC144140, BE833805, BM992381, DA933009

UniProtKB/TrEMBL

A0A8V8TMM6

Conserved Domains (5) summary

smart00608
Location:493 → 620

ACR; ADAM Cysteine-Rich Domain

cd04269
Location:204 → 397

ZnMc_adamalysin_II_like; Zinc-dependent metalloprotease; adamalysin_II_like subfamily. Adamalysin II is a snake venom zinc endopeptidase. This subfamily contains other snake venom metalloproteinases, as well as membrane-anchored metalloproteases belonging to the ADAM family. ...

pfam00200
Location:416 → 488

Disintegrin; Disintegrin

pfam01421
Location:204 → 399

Reprolysin; Reprolysin (M12B) family zinc metalloprotease

pfam01562
Location:40 → 155

Pep_M12B_propep; Reprolysin family propeptide
NM_014265.6 → NP_055080.2 disintegrin and metalloproteinase domain-containing protein 28 isoform 1 preproprotein

See identical proteins and their annotated locations for NP_055080.2

Status: REVIEWED

Description

Transcript Variant: This variant (1, also known as MDC-Lm) encodes the longest isoform (1).

Source sequence(s)

AC120193, AJ242015, BE833805, BM992381, DA933009

Consensus CDS

CCDS34865.1

UniProtKB/Swiss-Prot

B2RMV5, Q9UKQ2, Q9Y339, Q9Y3S0

UniProtKB/TrEMBL

A0A8V8TMM6

Related

ENSP00000265769.4, ENST00000265769.9

Conserved Domains (5) summary

smart00608
Location:493 → 620

ACR; ADAM Cysteine-Rich Domain

cd04269
Location:204 → 397

ZnMc_adamalysin_II_like; Zinc-dependent metalloprotease; adamalysin_II_like subfamily. Adamalysin II is a snake venom zinc endopeptidase. This subfamily contains other snake venom metalloproteinases, as well as membrane-anchored metalloproteases belonging to the ADAM family. ...

pfam00200
Location:416 → 488

Disintegrin; Disintegrin

pfam01421
Location:204 → 399

Reprolysin; Reprolysin (M12B) family zinc metalloprotease

pfam01562
Location:40 → 155

Pep_M12B_propep; Reprolysin family propeptide
NM_021777.5 → NP_068547.2 disintegrin and metalloproteinase domain-containing protein 28 isoform 3 preproprotein

See identical proteins and their annotated locations for NP_068547.2

Status: REVIEWED

Description

Transcript Variant: This variant (3, also known as MDC-Ls) lacks several exons and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 3 which is shorter and has a distinct C-terminus, compared to isoform 1.

Source sequence(s)

AC120193, AF137335, AJ242015, AK225921, DA933009

Consensus CDS

CCDS47830.1

UniProtKB/TrEMBL

A0A384NKT9

Related

ENSP00000393699.2, ENST00000437154.6

Conserved Domains (4) summary

smart00050
Location:416 → 490

DISIN; Homologues of snake disintegrins

cd04269
Location:204 → 397

ZnMc_adamalysin_II_like; Zinc-dependent metalloprotease; adamalysin_II_like subfamily. Adamalysin II is a snake venom zinc endopeptidase. This subfamily contains other snake venom metalloproteinases, as well as membrane-anchored metalloproteases belonging to the ADAM family. ...

pfam01562
Location:29 → 156

Pep_M12B_propep; Reprolysin family propeptide

cl15456
Location:493 → 531

ADAM_CR; ADAM cysteine-rich

RNA

NR_130709.2 RNA Sequence

Status: REVIEWED

Description

Transcript Variant: This variant (5) lacks several alternate exons and its 3' terminal exon extends past a splice site that is used in variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC120193, AF137335, AK293382, AK303437
NR_130710.2 RNA Sequence

Status: REVIEWED

Description

Transcript Variant: This variant (6) lacks one alternate internal exon, contains one alternate internal exon and uses an alternate splice site in an internal exon compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC120193, AJ242015, AK293382, AK303437, BC144140, BE833805, BM992381

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000008.11 Reference GRCh38.p14 Primary Assembly

Range

24294069..24359014

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_005273380.5 → XP_005273437.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X4

UniProtKB/TrEMBL

A0A8V8TMM6

Conserved Domains (5) summary

smart00608
Location:493 → 620

ACR; ADAM Cysteine-Rich Domain

cd04269
Location:204 → 397

ZnMc_adamalysin_II_like; Zinc-dependent metalloprotease; adamalysin_II_like subfamily. Adamalysin II is a snake venom zinc endopeptidase. This subfamily contains other snake venom metalloproteinases, as well as membrane-anchored metalloproteases belonging to the ADAM family. ...

pfam00200
Location:416 → 488

Disintegrin; Disintegrin

pfam01421
Location:204 → 399

Reprolysin; Reprolysin (M12B) family zinc metalloprotease

pfam01562
Location:40 → 155

Pep_M12B_propep; Reprolysin family propeptide
XM_017012974.3 → XP_016868463.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X3

UniProtKB/TrEMBL

A0A8V8TMM6
XM_011544368.4 → XP_011542670.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X6

See identical proteins and their annotated locations for XP_011542670.1

UniProtKB/TrEMBL

A0A8V8TMM6

Conserved Domains (5) summary

smart00608
Location:493 → 620

ACR; ADAM Cysteine-Rich Domain

cd04269
Location:204 → 397

ZnMc_adamalysin_II_like; Zinc-dependent metalloprotease; adamalysin_II_like subfamily. Adamalysin II is a snake venom zinc endopeptidase. This subfamily contains other snake venom metalloproteinases, as well as membrane-anchored metalloproteases belonging to the ADAM family. ...

pfam00200
Location:416 → 488

Disintegrin; Disintegrin

pfam01421
Location:204 → 399

Reprolysin; Reprolysin (M12B) family zinc metalloprotease

pfam01562
Location:40 → 155

Pep_M12B_propep; Reprolysin family propeptide
XM_017012975.3 → XP_016868464.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X5

UniProtKB/TrEMBL

A0A8V8TMM6
XM_006716273.4 → XP_006716336.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X1

UniProtKB/TrEMBL

A0A8V8TMM6

Conserved Domains (5) summary

smart00608
Location:493 → 620

ACR; ADAM Cysteine-Rich Domain

cd04269
Location:204 → 397

ZnMc_adamalysin_II_like; Zinc-dependent metalloprotease; adamalysin_II_like subfamily. Adamalysin II is a snake venom zinc endopeptidase. This subfamily contains other snake venom metalloproteinases, as well as membrane-anchored metalloproteases belonging to the ADAM family. ...

pfam00200
Location:416 → 488

Disintegrin; Disintegrin

pfam01421
Location:204 → 399

Reprolysin; Reprolysin (M12B) family zinc metalloprotease

pfam01562
Location:40 → 155

Pep_M12B_propep; Reprolysin family propeptide
XM_011544367.4 → XP_011542669.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X2

UniProtKB/TrEMBL

A0A8V8TMM6

Conserved Domains (5) summary

smart00608
Location:493 → 620

ACR; ADAM Cysteine-Rich Domain

cd04269
Location:204 → 397

ZnMc_adamalysin_II_like; Zinc-dependent metalloprotease; adamalysin_II_like subfamily. Adamalysin II is a snake venom zinc endopeptidase. This subfamily contains other snake venom metalloproteinases, as well as membrane-anchored metalloproteases belonging to the ADAM family. ...

pfam00200
Location:416 → 488

Disintegrin; Disintegrin

pfam01421
Location:204 → 399

Reprolysin; Reprolysin (M12B) family zinc metalloprotease

pfam01562
Location:40 → 155

Pep_M12B_propep; Reprolysin family propeptide
XM_047421270.1 → XP_047277226.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X4
XM_006716274.2 → XP_006716337.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X6

See identical proteins and their annotated locations for XP_006716337.1

UniProtKB/TrEMBL

A0A8V8TMM6

Conserved Domains (5) summary

smart00608
Location:493 → 620

ACR; ADAM Cysteine-Rich Domain

cd04269
Location:204 → 397

ZnMc_adamalysin_II_like; Zinc-dependent metalloprotease; adamalysin_II_like subfamily. Adamalysin II is a snake venom zinc endopeptidase. This subfamily contains other snake venom metalloproteinases, as well as membrane-anchored metalloproteases belonging to the ADAM family. ...

pfam00200
Location:416 → 488

Disintegrin; Disintegrin

pfam01421
Location:204 → 399

Reprolysin; Reprolysin (M12B) family zinc metalloprotease

pfam01562
Location:40 → 155

Pep_M12B_propep; Reprolysin family propeptide
XM_047421271.1 → XP_047277227.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X7
XM_011544369.3 → XP_011542671.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X9

UniProtKB/TrEMBL

A0A8V8TMM6

Conserved Domains (5) summary

smart00608
Location:493 → 620

ACR; ADAM Cysteine-Rich Domain

cd04269
Location:204 → 397

ZnMc_adamalysin_II_like; Zinc-dependent metalloprotease; adamalysin_II_like subfamily. Adamalysin II is a snake venom zinc endopeptidase. This subfamily contains other snake venom metalloproteinases, as well as membrane-anchored metalloproteases belonging to the ADAM family. ...

pfam00200
Location:416 → 488

Disintegrin; Disintegrin

pfam01421
Location:204 → 399

Reprolysin; Reprolysin (M12B) family zinc metalloprotease

pfam01562
Location:40 → 155

Pep_M12B_propep; Reprolysin family propeptide
XM_005273382.2 → XP_005273439.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X10

Conserved Domains (5) summary

smart00608
Location:493 → 610

ACR; ADAM Cysteine-Rich Domain

cd04269
Location:204 → 397

ZnMc_adamalysin_II_like; Zinc-dependent metalloprotease; adamalysin_II_like subfamily. Adamalysin II is a snake venom zinc endopeptidase. This subfamily contains other snake venom metalloproteinases, as well as membrane-anchored metalloproteases belonging to the ADAM family. ...

pfam00200
Location:416 → 488

Disintegrin; Disintegrin

pfam01421
Location:204 → 399

Reprolysin; Reprolysin (M12B) family zinc metalloprotease

pfam01562
Location:40 → 155

Pep_M12B_propep; Reprolysin family propeptide
XM_047421272.1 → XP_047277228.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X8
XM_047421273.1 → XP_047277229.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X11
XM_011544371.4 → XP_011542673.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X12

Conserved Domains (3) summary

smart00050
Location:163 → 237

DISIN; Homologues of snake disintegrins

smart00608
Location:240 → 367

ACR; ADAM Cysteine-Rich Domain

cd04269
Location:1 → 144

ZnMc_adamalysin_II_like; Zinc-dependent metalloprotease; adamalysin_II_like subfamily. Adamalysin II is a snake venom zinc endopeptidase. This subfamily contains other snake venom metalloproteinases, as well as membrane-anchored metalloproteases belonging to the ADAM family. ...
XM_047421274.1 → XP_047277230.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X13

Related

ENSP00000429484.1, ENST00000521629.5

Alternate T2T-CHM13v2.0

Genomic

NC_060932.1 Alternate T2T-CHM13v2.0

Range

24569171..24634154

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_054359601.1 → XP_054215576.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X4
XM_054359599.1 → XP_054215574.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X3
XM_054359604.1 → XP_054215579.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X6
XM_054359602.1 → XP_054215577.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X5
XM_054359597.1 → XP_054215572.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X1
XM_054359598.1 → XP_054215573.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X2
XM_054359600.1 → XP_054215575.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X4
XM_054359603.1 → XP_054215578.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X6
XM_054359605.1 → XP_054215580.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X7
XM_054359607.1 → XP_054215582.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X9
XM_054359608.1 → XP_054215583.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X10
XM_054359606.1 → XP_054215581.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X8
XM_054359609.1 → XP_054215584.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X11
XM_054359610.1 → XP_054215585.1 disintegrin and metalloproteinase domain-containing protein 28 isoform X12