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Chrm4 cholinergic receptor, muscarinic 4 [ Mus musculus (house mouse) ]

Gene ID: 12672, updated on 2-Nov-2024

Summary

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Official Symbol
Chrm4provided by MGI
Official Full Name
cholinergic receptor, muscarinic 4provided by MGI
Primary source
MGI:MGI:88399
See related
Ensembl:ENSMUSG00000040495 AllianceGenome:MGI:88399
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
M4; Chrm-4; AChR M4; mm4 mAChR
Summary
Enables G protein-coupled acetylcholine receptor activity. Acts upstream of or within adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway. Located in membrane. Is expressed in several structures, including alimentary system; eye; limb; nervous system; and reproductive system. Orthologous to human CHRM4 (cholinergic receptor muscarinic 4). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Biased expression in frontal lobe adult (RPKM 11.8), testis adult (RPKM 10.1) and 6 other tissues See more
Orthologs
human all
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Genomic context

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See Chrm4 in Genome Data Viewer
Location:
2 E1; 2 50.63 cM
Exon count:
2
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (91752531..91760180)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (91922189..91929835)

Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_05122 Neighboring gene harbinger transposase derived 1 Neighboring gene dyskeratosis congenita 1, dyskerin homolog pseudogene Neighboring gene autophagy/beclin 1 regulator 1 Neighboring gene STARR-seq mESC enhancer starr_05123 Neighboring gene predicted gene, 39876 Neighboring gene diacylglycerol kinase zeta Neighboring gene midkine Neighboring gene microRNA 6999

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Vagal-mAChR4 signaling promotes Friend virus complex (FV)-induced acute erythroleukemia. Song S, et al. Virol Sin, 2023 Jun. PMID 37172825, Free PMC Article
  2. Deletion analysis of the m4 muscarinic acetylcholine receptor. Molecular determinants for activation of but not coupling to the Gi guanine-nucleotide-binding regulatory protein regulate receptor internalization. Van Koppen CJ, et al. Eur J Biochem, 1994 Jun 1. PMID 8020490
  3. Enhancement of D1 dopamine receptor-mediated locomotor stimulation in M(4) muscarinic acetylcholine receptor knockout mice. Gomeza J, et al. Proc Natl Acad Sci U S A, 1999 Aug 31. PMID 10468635, Free PMC Article
  4. The M4 muscarinic receptor-selective effects on keratinocyte crawling locomotion. Chernyavsky AI, et al. Life Sci, 2003 Mar 28. PMID 12628458
  5. Loss of anti-cataleptic effect of scopolamine in mice lacking muscarinic acetylcholine receptor subtype 4. Karasawa H, et al. Eur J Pharmacol, 2003 May 2. PMID 12729838

See all (103) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Vagal-mAChR4 signaling promotes Friend virus complex (FV)-induced acute erythroleukemia.
    Title: Vagal-mAChR4 signaling promotes Friend virus complex (FV)-induced acute erythroleukemia.
  2. Revealing a compulsive phenotype in cholinergic M4(-/-) mice depends on the inter-trial interval initiation settings in a five choice serial reaction time task.
    Title: Revealing a compulsive phenotype in cholinergic M(4)(-/-) mice depends on the inter-trial interval initiation settings in a five choice serial reaction time task.
  3. Lack of M4 muscarinic receptors in the striatum, thalamus and intergeniculate leaflet alters the biological rhythm of locomotor activity in mice.
    Title: Lack of M(4) muscarinic receptors in the striatum, thalamus and intergeniculate leaflet alters the biological rhythm of locomotor activity in mice.
  4. The Firing of Theta State-Related Septal Cholinergic Neurons Disrupt Hippocampal Ripple Oscillations via Muscarinic Receptors.
    Title: The Firing of Theta State-Related Septal Cholinergic Neurons Disrupt Hippocampal Ripple Oscillations via Muscarinic Receptors.
  5. Muscarinic acetylcholine receptor regulates self-renewal of early erythroid progenitors.
    Title: Muscarinic acetylcholine receptor regulates self-renewal of early erythroid progenitors.
  6. Muscarinic receptors (MR) are typical members of G protein-coupled receptors and can be divided into five subtypes (M1-M5), which activate different G proteins.
    Title: The deletion of M(4) muscarinic receptors increases motor activity in females in the dark phase.
  7. The findings of this study indicated that the cocaine-like S(D) effects of muscarinic antagonists are primarily mediated through M1 receptors, with a minor contribution of M4 receptors.
    Title: Effects of muscarinic receptor antagonists on cocaine discrimination in wild-type mice and in muscarinic receptor M(1), M(2), and M(4) receptor knockout mice.
  8. The three receptor sets considered (mAChR, AR and TrkB receptors) intervene in modulating the conditions of the competition between nerve endings.
    Title: Presynaptic muscarinic acetylcholine autoreceptors (M1, M2 and M4 subtypes), adenosine receptors (A1 and A2A) and tropomyosin-related kinase B receptor (TrkB) modulate the developmental synapse elimination process at the neuromuscular junction.
  9. In the present study, the functional coupling of Gi/o protein-coupled receptors to GalR1, and the CB1 receptor subtype for endocannabinoids were analyzed in the 3xTg-AD mice model of Alzheimer's disease in the prodromal and advanced stages. In addition, the activity mediated by Gi/o protein-coupled M2/4 muscarinic receptor subtypes was also analyzed in brain areas involved in anxiety and cognition.
    Title: Activity of muscarinic, galanin and cannabinoid receptors in the prodromal and advanced stages in the triple transgenic mice model of Alzheimer's disease.
  10. Muscarinic acetylcholine receptor subtype 4 is essential for cholinergic stimulation of duodenal bicarbonate secretion in mice - relationship to D cell/somatostatin
    Title: Muscarinic acetylcholine receptor subtype 4 is essential for cholinergic stimulation of duodenal bicarbonate secretion in mice - relationship to D cell/somatostatin.
Submit: New GeneRIF Correction See all GeneRIFs (31)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables G protein-coupled acetylcholine receptor activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables G protein-coupled acetylcholine receptor activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables guanyl-nucleotide exchange factor activity ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in chemical synaptic transmission IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of locomotion IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in asymmetric synapse ISO
Inferred from Sequence Orthology
more info
  
located_in axon terminus ISO
Inferred from Sequence Orthology
more info
  
is_active_in dendrite IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in glutamatergic synapse ISO
Inferred from Sequence Orthology
more info
  
located_in membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in neuronal cell body ISO
Inferred from Sequence Orthology
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in postsynaptic density membrane ISO
Inferred from Sequence Orthology
more info
  
located_in postsynaptic membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in presynaptic membrane ISO
Inferred from Sequence Orthology
more info
  
located_in sarcolemma ISO
Inferred from Sequence Orthology
more info
  
is_active_in synapse IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
muscarinic acetylcholine receptor M4
Names
muscarinic acetylcholine receptor 4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001416938.1 → NP_001403867.1  muscarinic acetylcholine receptor M4

    Status: VALIDATED

    Source sequence(s)
    AL731772
    UniProtKB/Swiss-Prot
    P32211, Q64056
    UniProtKB/TrEMBL
    Q0VBU3

  2. NM_007699.3 → NP_031725.1  muscarinic acetylcholine receptor M4

    See identical proteins and their annotated locations for NP_031725.1

    Status: VALIDATED

    Source sequence(s)
    AL731772
    Consensus CDS
    CCDS16439.1
    UniProtKB/Swiss-Prot
    P32211, Q64056
    UniProtKB/TrEMBL
    Q0VBU3
    Related
    ENSMUSP00000040808.4, ENSMUST00000045537.4
    Conserved Domains (2) summary
    pfam00001
    Location:48 → 453
    7tm_1; 7 transmembrane receptor (rhodopsin family)
    cl21561
    Location:40 → 172
    7tm_4; Olfactory receptor

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000068.8 Reference GRCm39 C57BL/6J

    Range
    91752531..91760180
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P32211.1 GenPept UniProtKB/Swiss-Prot:P32211

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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