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Afap1 actin filament associated protein 1 [ Rattus norvegicus (Norway rat) ]

Gene ID: 140935, updated on 6-Jun-2024

Summary

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Official Symbol
Afap1provided by RGD
Official Full Name
actin filament associated protein 1provided by RGD
Primary source
RGD:619959
See related
Ensembl:ENSRNOG00000060665 AllianceGenome:RGD:619959
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as
Afap
Summary
Enables SH2 domain binding activity and SH3 domain binding activity. Involved in cellular response to mechanical stimulus and regulation of signal transduction. Located in actin filament. Human ortholog(s) of this gene implicated in open-angle glaucoma. Orthologous to human AFAP1 (actin filament associated protein 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in Heart (RPKM 69.0), Kidney (RPKM 49.5) and 9 other tissues See more
Orthologs
human mouse all
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Genomic context

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See Afap1 in Genome Data Viewer
Location:
14q21
Exon count:
19
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 14 NC_086032.1 (78967961..79081032, complement)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 14 NC_051349.1 (74743322..74856300, complement)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 14 NC_005113.4 (79923132..80035096, complement)

Chromosome 14 - NC_086032.1Genomic Context describing neighboring genes Neighboring gene sortilin-related VPS10 domain containing receptor 2 Neighboring gene uncharacterized LOC134481778 Neighboring gene prosaposin-like 1 Neighboring gene uncharacterized LOC134481856 Neighboring gene uncharacterized LOC134481855 Neighboring gene actin binding LIM protein family, member 2

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. Phosphorylation of AFAP-110 affects podosome lifespan in A7r5 cells. Dorfleutner A, et al. J Cell Sci, 2008 Jul 15. PMID 18577577
  2. Molecular cloning of actin filament-associated protein: a putative adaptor in stretch-induced Src activation. Lodyga M, et al. Am J Physiol Lung Cell Mol Physiol, 2002 Aug. PMID 12114187
  3. AFAP1 Is a Novel Downstream Mediator of TGF-β1 for CCN2 Induction in Osteoblasts. Cho Y, et al. PLoS One, 2015. PMID 26340021, Free PMC Article
  4. Tks5 recruits AFAP-110, p190RhoGAP, and cortactin for podosome formation. Crimaldi L, et al. Exp Cell Res, 2009 Sep 10. PMID 19540230
  5. Common variants near ABCA1, AFAP1 and GMDS confer risk of primary open-angle glaucoma. Gharahkhani P, et al. Nat Genet, 2014 Oct. PMID 25173105, Free PMC Article

See all (7) citations in PubMed

GeneRIFs: Gene References Into Functions

General gene information

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Markers

Homology

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables SH2 domain binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables SH2 domain binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables SH3 domain binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables SH3 domain binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables actin binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in cellular response to mechanical stimulus IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in regulation of signal transduction IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in actin cytoskeleton IEA
Inferred from Electronic Annotation
more info
  
located_in actin cytoskeleton ISO
Inferred from Sequence Orthology
more info
  
located_in actin filament IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in focal adhesion IEA
Inferred from Electronic Annotation
more info
  
located_in focal adhesion ISO
Inferred from Sequence Orthology
more info
  
located_in stress fiber IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
actin filament-associated protein 1
Names
110 kDa actin filament-associated protein
AFAP-110

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_080900.2 → NP_543176.2  actin filament-associated protein 1

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000014
    UniProtKB/Swiss-Prot
    Q8VH46
    UniProtKB/TrEMBL
    A0A0G2JVI5, A6IJY7
    Related
    ENSRNOP00000069497.2, ENSRNOT00000090597.2
    Conserved Domains (3) summary
    cd13306
    Location:142 → 248
    PH1_AFAP; Actin filament associated protein family Pleckstrin homology (PH) domain, repeat 1
    cd13307
    Location:348 → 448
    PH2_AFAP; Actin filament associated protein family Pleckstrin homology (PH) domain, repeat 2
    pfam08317
    Location:561 → 649
    Spc7; Spc7 kinetochore protein

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086032.1 Reference GRCr8

    Range
    78967961..79081032 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_063272812.1 → XP_063128882.1  actin filament-associated protein 1 isoform X5

    UniProtKB/Swiss-Prot
    Q8VH46

  2. XM_008770247.4 → XP_008768469.1  actin filament-associated protein 1 isoform X2

    UniProtKB/Swiss-Prot
    Q8VH46
    UniProtKB/TrEMBL
    A0A8I6ALM6
    Related
    ENSRNOP00000095531.1
    Conserved Domains (4) summary
    cd13306
    Location:142 → 248
    PH1_AFAP; Actin filament associated protein family Pleckstrin homology (PH) domain, repeat 1
    cd13307
    Location:348 → 448
    PH2_AFAP; Actin filament associated protein family Pleckstrin homology (PH) domain, repeat 2
    pfam00169
    Location:154 → 246
    PH; PH domain
    pfam10211
    Location:635 → 707
    Ax_dynein_light; Axonemal dynein light chain

  3. XM_039091572.2 → XP_038947500.1  actin filament-associated protein 1 isoform X3

    UniProtKB/Swiss-Prot
    Q8VH46
    Conserved Domains (3) summary
    cd13306
    Location:142 → 248
    PH1_AFAP; Actin filament associated protein family Pleckstrin homology (PH) domain, repeat 1
    cd13307
    Location:348 → 448
    PH2_AFAP; Actin filament associated protein family Pleckstrin homology (PH) domain, repeat 2
    pfam08317
    Location:650 → 738
    Spc7; Spc7 kinetochore protein

  4. XM_063272813.1 → XP_063128883.1  actin filament-associated protein 1 isoform X6

    UniProtKB/Swiss-Prot
    Q8VH46

  5. XM_063272815.1 → XP_063128885.1  actin filament-associated protein 1 isoform X8

    UniProtKB/Swiss-Prot
    Q8VH46
    UniProtKB/TrEMBL
    G3V6Z3

  6. XM_008770245.4 → XP_008768467.1  actin filament-associated protein 1 isoform X7

    UniProtKB/Swiss-Prot
    Q8VH46
    Related
    ENSRNOP00000069806.2
    Conserved Domains (4) summary
    cd13306
    Location:142 → 248
    PH1_AFAP; Actin filament associated protein family Pleckstrin homology (PH) domain, repeat 1
    cd13307
    Location:348 → 448
    PH2_AFAP; Actin filament associated protein family Pleckstrin homology (PH) domain, repeat 2
    pfam00169
    Location:154 → 246
    PH; PH domain
    pfam10211
    Location:546 → 618
    Ax_dynein_light; Axonemal dynein light chain

  7. XM_039091573.2 → XP_038947501.1  actin filament-associated protein 1 isoform X4

    UniProtKB/Swiss-Prot
    Q8VH46
    Conserved Domains (3) summary
    cd13306
    Location:142 → 248
    PH1_AFAP; Actin filament associated protein family Pleckstrin homology (PH) domain, repeat 1
    cd13307
    Location:348 → 448
    PH2_AFAP; Actin filament associated protein family Pleckstrin homology (PH) domain, repeat 2
    pfam08317
    Location:579 → 667
    Spc7; Spc7 kinetochore protein

  8. XM_017599057.3 → XP_017454546.1  actin filament-associated protein 1 isoform X1

    UniProtKB/Swiss-Prot
    Q8VH46
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8VH46.1 GenPept UniProtKB/Swiss-Prot:Q8VH46

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