lin-35 Retinoblastoma-like protein homolog lin-35 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: lin-35
Official Full Name: Retinoblastoma-like protein homolog lin-35
Primary source: WormBase:WBGene00003020
Locus tag: CELE_C32F10.2
See related: AllianceGenome:WB:WBGene00003020
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Predicted to enable RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Involved in several processes, including negative regulation of cell cycle; negative regulation of vulval development; and positive regulation of brood size. Located in nucleus. Is expressed in several structures, including head and tail. Used to study intellectual disability. Human ortholog(s) of this gene implicated in several diseases, including high grade glioma (multiple); osteosarcoma; prostate cancer; retinoblastoma (multiple); and small cell carcinoma. Orthologous to several human genes including RBL1 (RB transcriptional corepressor like 1) and RBL2 (RB transcriptional corepressor like 2). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See lin-35 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 10

Sequence:: Chromosome: I; NC_003279.8 (5807709..5815637)

Chromosome I - NC_003279.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003279.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

LIN-35 is necessary in both the soma and germline for preserving fertility in Caenorhabditis elegans under moderate temperature stress.
Title: LIN-35 is necessary in both the soma and germline for preserving fertility in Caenorhabditis elegans under moderate temperature stress.
LIN-35 beyond its classical roles: its function in the stress response.
Title: LIN-35 beyond its classical roles: its function in the stress response.
Study reports that LIN-35 indeed mediates the association of E2F-DP and MuvB, a function that stabilizes (Dp/Retinoblastoma(Rb)-like/E2F/MuvB) or DREAM transcriptional repressor complex subunit occupancy at target genes. In the absence of LIN-35, the occupancy of E2F-DP and MuvB at most DREAM target genes decreases dramatically and many of those genes become upregulated.
Title: Loss of the Caenorhabditis elegans pocket protein LIN-35 reveals MuvB's innate function as the repressor of DREAM target genes.
Simultaneous mutation of lin-35 and fzr-1, an orthologue to Cdh1, completely eliminates the essential requirement of CDK4/6-cyclin D (CDK-4/CYD-1) in C. elegans.
Title: Rb and FZR1/Cdh1 determine CDK4/6-cyclin D requirement in C. elegans and human cancer cells.
CED-9/Bcl-2 downregulation via LIN-35/Rb triggers germ cell apoptosis in C. elegans in response to starvation.
Title: LIN-35/Rb causes starvation-induced germ cell apoptosis via CED-9/Bcl2 downregulation in Caenorhabditis elegans.
Tumor suppressor Rb maintains the "starvation-induced" transcriptome and represses the "refeeding-induced" transcriptome, including the repression of many pathogen-, toxin-, and oxidative-stress-inducible and metabolic genes.
Title: The tumor suppressor Rb critically regulates starvation-induced stress response in C. elegans.
The function of LIN-35/Rb is to prevent germline gene expression in the soma
Title: Repression of germline RNAi pathways in somatic cells by retinoblastoma pathway chromatin complexes.
mir-35-41 miRNA gene inhibits the exogenous RNAi pathway by positively regulating the expression of LIN-35/Rb protein
Title: The miR-35-41 family of microRNAs regulates RNAi sensitivity in Caenorhabditis elegans.
LIN-35 and UBC-18 act through distinct mechanisms to negatively regulate SUP-35 expression.
Title: A mechanistic basis for the coordinated regulation of pharyngeal morphogenesis in Caenorhabditis elegans by LIN-35/Rb and UBC-18-ARI-1.
both LIN-35 and SLR-2 act in the intestine to regulate the expression of many genes required for normal nutrient utilization
Title: Coordinated regulation of intestinal functions in C. elegans by LIN-35/Rb and SLR-2.

Submit: New GeneRIF Correction See all GeneRIFs (24)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

B0414.8 (e-PCR)
- UniSTS:308387

C32F10.2 (e-PCR)
- UniSTS:309214

C32F10.4 (e-PCR)
- UniSTS:309215

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cell cycle	IEA Inferred from Electronic Annotation more info
involved_in cell differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in embryo development ending in birth or egg hatching	IGI Inferred from Genetic Interaction more info	PubMed
involved_in embryonic digestive tract morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in gonad development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of DNA endoreduplication	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of G1/S transition of mitotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of cell cycle G1/S phase transition	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of mitotic cell cycle	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of transcription by RNA polymerase I	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in negative regulation of vulval development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in nematode larval development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in nematode larval development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of brood size	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of multicellular organism growth	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of cell cycle	IEA Inferred from Electronic Annotation more info
involved_in regulation of cell cycle G1/S phase transition	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of cell division	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in reproductive process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in reproductive process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chromatin	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of transcription regulator complex	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Retinoblastoma-like protein homolog lin-35

NP_491686.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003279.8 Reference assembly

Range

5807709..5815637

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_059285.4 → NP_491686.1 Retinoblastoma-like protein homolog lin-35 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_491686.1

Status: REVIEWED

UniProtKB/Swiss-Prot

G5EDT1

Conserved Domains (4) summary

pfam01857
Location:744 → 888

RB_B; Retinoblastoma-associated protein B domain

pfam01858
Location:507 → 690

RB_A; Retinoblastoma-associated protein A domain

pfam11934
Location:200 → 312

DUF3452; Domain of unknown function (DUF3452)

cl27354
Location:339 → 408

HIS2; Histidinol phosphatase or related hydrolase of the PHP family [Amino acid transport and metabolism, General function prediction only]