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Mest mesoderm specific transcript [ Mus musculus (house mouse) ]

Gene ID: 17294, updated on 28-Oct-2024

Summary

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Official Symbol
Mestprovided by MGI
Official Full Name
mesoderm specific transcriptprovided by MGI
Primary source
MGI:MGI:96968
See related
Ensembl:ENSMUSG00000051855 AllianceGenome:MGI:96968
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Peg1
Summary
Predicted to enable hydrolase activity. Involved in regulation of lipid storage and response to retinoic acid. Located in endoplasmic reticulum. Is expressed in several structures, including central nervous system; embryo mesenchyme; extraembryonic component; heart and pericardium; and neural retina. Used to study Barth syndrome. Orthologous to human MEST (mesoderm specific transcript). [provided by Alliance of Genome Resources, Oct 2024]
Expression
Biased expression in limb E14.5 (RPKM 597.0), CNS E11.5 (RPKM 207.7) and 9 other tissues See more
Orthologs
human all
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Genomic context

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See Mest in Genome Data Viewer
Location:
6 A3.3; 6 12.53 cM
Exon count:
15
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 6 NC_000072.7 (30733505..30783580)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 6 NC_000072.6 (30733506..30748466)

Chromosome 6 - NC_000072.7Genomic Context describing neighboring genes Neighboring gene carboxypeptidase A1, pancreatic Neighboring gene centrosomal protein 41 Neighboring gene predicted gene 13782 Neighboring gene STARR-positive B cell enhancer mm9_chr6:30643637-30643938 Neighboring gene STARR-seq mESC enhancer starr_15474 Neighboring gene microRNA 335 Neighboring gene coatomer protein complex, subunit gamma 2 Neighboring gene coatomer protein complex, subunit gamma 2, opposite strand 2 Neighboring gene RIKEN cDNA 4930412F09 gene Neighboring gene predicted gene, 38781 Neighboring gene testis specific gene A13

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Mest/Peg1 imprinted gene enlarges adipocytes and is a marker of adipocyte size. Takahashi M, et al. Am J Physiol Endocrinol Metab, 2005 Jan. PMID 15353408
  2. Imprinted mesodermal specific transcript (MEST) and H19 genes in renal development and diabetes. Kanwar YS, et al. Kidney Int, 2003 May. PMID 12675841
  3. Peg1/Mest, an imprinted gene, is involved in mammary gland maturation. Yonekura S, et al. J Cell Physiol, 2019 Feb. PMID 30144363
  4. Mesoderm-specific transcript localization in the ER and ER-lipid droplet interface supports a role in adipocyte hypertrophy. Prudovsky I, et al. J Cell Biochem, 2018 Mar. PMID 29058774, Free PMC Article
  5. Expression of Peg1 (Mest) in the developing mouse heart: involvement in trabeculation. King T, et al. Dev Dyn, 2002 Oct. PMID 12242721

See all (132) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The paternally imprinted gene Peg1/Mest is involved in mammary alveoli formation.
    Title: Peg1/Mest, an imprinted gene, is involved in mammary gland maturation.
  2. Identification of MEST as an endoplasmic reticulum-specific protein that co-localizes with lipid droplets in cells undergoing adipogenic differentiation supports a function for MEST in the facilitation of lipid accumulation and storage in adipocytes.
    Title: Mesoderm-specific transcript localization in the ER and ER-lipid droplet interface supports a role in adipocyte hypertrophy.
  3. This study demonstrated that the Mest and its intron product, miR-335, play important roles in neuronal migration with Mest regulating the morphological transition of primary neurons required in the formation of the mammalian neocortex.
    Title: Defective neuronal migration and inhibition of bipolar to multipolar transition of migrating neural cells by Mesoderm-Specific Transcript, Mest, in the developing mouse neocortex.
  4. diet-induced ATE in MEST-deficient mice diminishes hypoxia and inflammation in WAT leading to improved glucose tolerance and insulin sensitivity
    Title: Diet-induced adipose tissue expansion is mitigated in mice with a targeted inactivation of mesoderm specific transcript (Mest).
  5. The androgenetic patterns of H19, Snrpn, and Mest were maintained even after differentiation of germline-derived pluripotent stem cells(gPS) into neural stem cells(NSC), whereas the fully unmethylated status of Ndn in SSCs was altered to somatic patterns in gPS cells and gPS-NSCs.
    Title: Epigenetic alteration of imprinted genes during neural differentiation of germline-derived pluripotent stem cells.
  6. The balance of factors controlling fat deposition can be evaluated in part by the differential expression profiles of Mest and Sfrp5 genes with functions linked to fat deposition as long as there is an active accumulation of fat mass.
    Title: Mest and Sfrp5 are biomarkers for healthy adipose tissue.
  7. the expression of Mest and Sfrp5 were tightly associated across the 5 mouse strains with the highest and lowest expression occurring in DBA/2J and C57BL/6J (B6) respectively suggesting a common mechanism for their regulation.
    Title: Adipose tissue Mest and Sfrp5 are concomitant with variations of adiposity among inbred mouse strains fed a non-obesogenic diet.
  8. Although both Mest and miR-335 are highly expressed during muscle development and regeneration, only Mest+/- (maternal/paternal) mice show retardation of body growth.
    Title: Mest but Not MiR-335 Affects Skeletal Muscle Growth and Regeneration.
  9. The paternally expressed imprinted gene Mest was only detected in haploid androgenetic blastocysts and was significantly lower in this group relative to the controls, indicating that the gene maintains expression patterns specific to its parental origin.
    Title: Analysis of imprinted gene expression and implantation in haploid androgenetic mouse embryos.
  10. analysis of modifications of H3 histone in H19 and MEST imprinted genes in blastocysts produced in vitro from non-vitrified and vitrified two-cell embryos
    Title: H19 and MEST gene expression and histone modification in blastocysts cultured from vitrified and fresh two-cell mouse embryos.
Submit: New GeneRIF Correction See all GeneRIFs (24)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (3) 
  • Targeted (3)  1 citation

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in regulation of lipid storage IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in response to retinoic acid IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
is_active_in endoplasmic reticulum IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in endoplasmic reticulum IDA
Inferred from Direct Assay
more info
 PubMed 
located_in endoplasmic reticulum membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
mesoderm-specific transcript protein
Names
paternally-expressed gene 1 protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001252292.1 → NP_001239221.1  mesoderm-specific transcript protein isoform 1

    See identical proteins and their annotated locations for NP_001239221.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform 1).
    Source sequence(s)
    AK146457, AK168732, AK169266, BF608470
    Consensus CDS
    CCDS80512.1
    UniProtKB/TrEMBL
    D3Z383
    Related
    ENSMUSP00000117713.3, ENSMUST00000124665.3
    Conserved Domains (1) summary
    COG0596
    Location:54 → 328
    MhpC; Pimeloyl-ACP methyl ester carboxylesterase [Coenzyme transport and metabolism, General function prediction only]

  2. NM_001252293.1 → NP_001239222.1  mesoderm-specific transcript protein isoform 1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) has a different 5' end, compared to variant 1. The resulting protein (isoform 3) has a shorter N-terminus when it is compared to isoform 1.
    Source sequence(s)
    AF482999, AK146457, BF608470, CF916557
    Consensus CDS
    CCDS80513.1
    UniProtKB/Swiss-Prot
    Q07646
    Related
    ENSMUSP00000119038.3, ENSMUST00000157040.8
    Conserved Domains (1) summary
    COG0596
    Location:38 → 312
    MhpC; Pimeloyl-ACP methyl ester carboxylesterase [Coenzyme transport and metabolism, General function prediction only]

  3. NM_008590.2 → NP_032616.1  mesoderm-specific transcript protein isoform 2

    See identical proteins and their annotated locations for NP_032616.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1.
    Source sequence(s)
    AK032881, BF608470, CJ162886
    Consensus CDS
    CCDS19979.1
    UniProtKB/Swiss-Prot
    Q07646, Q792T8, Q8BS88, Q8K463, Q99KT5
    Related
    ENSMUSP00000129639.3, ENSMUST00000163949.9
    Conserved Domains (1) summary
    COG0596
    Location:47 → 321
    MhpC; Pimeloyl-ACP methyl ester carboxylesterase [Coenzyme transport and metabolism, General function prediction only]

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000072.7 Reference GRCm39 C57BL/6J

    Range
    30733505..30783580
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036165900.1 → XP_036021793.1  mesoderm-specific transcript protein isoform X2

    UniProtKB/Swiss-Prot
    Q07646, Q792T8, Q8BS88, Q8K463, Q99KT5
    Conserved Domains (1) summary
    COG0596
    Location:47 → 321
    MhpC; Pimeloyl-ACP methyl ester carboxylesterase [Coenzyme transport and metabolism, General function prediction only]

  2. XM_036165902.1 → XP_036021795.1  mesoderm-specific transcript protein isoform X4

    Conserved Domains (1) summary
    COG0596
    Location:47 → 295
    MhpC; Pimeloyl-ACP methyl ester carboxylesterase [Coenzyme transport and metabolism, General function prediction only]

  3. XM_036165901.1 → XP_036021794.1  mesoderm-specific transcript protein isoform X3

    Conserved Domains (1) summary
    COG0596
    Location:54 → 302
    MhpC; Pimeloyl-ACP methyl ester carboxylesterase [Coenzyme transport and metabolism, General function prediction only]

  4. XM_036165899.1 → XP_036021792.1  mesoderm-specific transcript protein isoform X1

    UniProtKB/TrEMBL
    D3Z383
    Conserved Domains (1) summary
    COG0596
    Location:54 → 328
    MhpC; Pimeloyl-ACP methyl ester carboxylesterase [Coenzyme transport and metabolism, General function prediction only]
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q07646.1 GenPept UniProtKB/Swiss-Prot:Q07646

Gene LinkOut

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