fbf-2 Fem-3 mRNA-binding factor 2 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: fbf-2
Official Full Name: Fem-3 mRNA-binding factor 2
Primary source: WormBase:WBGene00001402
Locus tag: CELE_F21H12.5
See related: AllianceGenome:WB:WBGene00001402
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables mRNA 3'-UTR binding activity. Involved in negative regulation of translation. Located in P granule. Is expressed in germ line. Used to study obesity. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See fbf-2 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 8

Sequence:: Chromosome: II; NC_003280.10 (6088863..6091480, complement)

Chromosome II - NC_003280.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003280.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

COP9 signalosome component CSN-5 stabilizes PUF proteins FBF-1 and FBF-2 in Caenorhabditis elegans germline stem and progenitor cells.
Title: COP9 signalosome component CSN-5 stabilizes PUF proteins FBF-1 and FBF-2 in Caenorhabditis elegans germline stem and progenitor cells.
Antagonistic control of Caenorhabditis elegans germline stem cell proliferation and differentiation by PUF proteins FBF-1 and FBF-2.
Title: Antagonistic control of Caenorhabditis elegans germline stem cell proliferation and differentiation by PUF proteins FBF-1 and FBF-2.
LST-1 weakens FBF-2 binding affinity for short and long motifs, which may increase target selectivity.
Title: A crystal structure of a collaborative RNA regulatory complex reveals mechanisms to refine target specificity.
Data indicate that a 10-amino-acid region in germline development defective-3 (GLD-3) is required for fem-3 binding factor (FBF) binding, while a 7-amino-acid loop in FBF between PUF repeats 7 and 8 is necessary for GLD-3 binding.
Title: A protein.protein interaction platform involved in recruitment of GLD-3 to the FBF.fem-3 mRNA complex.
Data show that CPB-1 (cytoplasmic polyadenylation element binding protein homolog-1) alters the affinity of FBF (fem-3 mRNA binding factor) for specific RNA sequences.
Title: Biochemical characterization of the Caenorhabditis elegans FBF.CPB-1 translational regulation complex identifies conserved protein interaction hotspots.
findings show FBF-1 and FBF-2 have different effects on target mRNAs; FBF-2 prevents translation; FBF-2 activity depends on PGL-1; PGL-1 is required to localize FBF-2 to perinuclear P granules and for efficient binding of FBF-2 to its mRNA targets
Title: The P granule component PGL-1 promotes the localization and silencing activity of the PUF protein FBF-2 in germline stem cells.
Data suggest that the FBF-2/GLD-2 protein directed polyadenylation of the endogenous mRNA, compensating for that mRNA's natural deadenylation.
Title: Targeted translational regulation using the PUF protein family scaffold.
The Puf RNA-binding proteins FBF-1 and FBF-2 inhibit the expression of synaptonemal complex proteins in germline stem cells.
Title: The Puf RNA-binding proteins FBF-1 and FBF-2 inhibit the expression of synaptonemal complex proteins in germline stem cells.
fbf-2 regulates the size of the mitotic region in helminth germ cells.
Title: FBF-1 and FBF-2 regulate the size of the mitotic region in the C. elegans germline.
FBF may coordinately repress sperm-specifying mRNAs to direct oogenesis and it represses the fog-1 mRNA to maintain FOG-1 at a low level appropriate for proliferation
Title: Dose-dependent control of proliferation and sperm specification by FOG-1/CPEB.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

F21H12.5 (e-PCR)
- UniSTS:309877

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables mRNA 3'-UTR binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA 3'-UTR binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of translation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in post-transcriptional regulation of gene expression	IBA Inferred from Biological aspect of Ancestor more info
involved_in sex differentiation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in P granule	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Fem-3 mRNA-binding factor 2

NP_001367592.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003280.10 Reference assembly

Range

6088863..6091480 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001381445.2 → NP_001367592.1 Fem-3 mRNA-binding factor 2 [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/Swiss-Prot

Q09312

Conserved Domains (1) summary

cd07920
Location:182 → 562

Pumilio; Pumilio-family RNA binding domain