wrn-1 ATP-dependent helicase wrn-1 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: wrn-1
Official Full Name: ATP-dependent helicase wrn-1
Primary source: WormBase:WBGene00006944
Locus tag: CELE_F18C5.2
See related: AllianceGenome:WB:WBGene00006944
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables 3'-5' DNA helicase activity. Involved in DNA metabolic process; determination of adult lifespan; and response to ionizing radiation. Located in nucleoplasm and site of double-strand break. Is expressed in several structures, including germ line and hypodermis. Used to study Werner syndrome. Human ortholog(s) of this gene implicated in Werner syndrome; breast cancer; coronary artery disease (multiple); diffuse scleroderma; and senile cataract. Orthologous to human WRN (WRN RecQ like helicase). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See wrn-1 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 16

Sequence:: Chromosome: II; NC_003280.10 (6554977..6559263)

Chromosome II - NC_003280.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003280.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

MUT-7 Provides Molecular Insight into the Werner Syndrome Exonuclease.
Title: MUT-7 Provides Molecular Insight into the Werner Syndrome Exonuclease.
WRN-1 plays a conserved role in the resection of DSB ends and mediates checkpoint signaling, thereby influencing levels of RPA and RAD-51
Title: The Caenorhabditis elegans WRN helicase promotes double-strand DNA break repair by mediating end resection and checkpoint activation.
WRN-1 functions downstream of RPA-1 and upstream of CHK-1 in the DSB checkpoint pathway and is also required for the repair of DSB.
Title: The Caenorhabditis elegans Werner syndrome protein participates in DNA damage checkpoint and DNA repair in response to CPT-induced double-strand breaks.
analysis of physical and functional interactions of Caenorhabditis elegans WRN-1 helicase with RPA-1
Title: Physical and functional interactions of Caenorhabditis elegans WRN-1 helicase with RPA-1.
WRN-1 functions together with RPA-1 upstream of ATM and ATR to trigger cell cycle arrest in response to double-strand DNA breaks.
Title: The Caenorhabditis elegans Werner syndrome protein functions upstream of ATR and ATM in response to DNA replication inhibition and double-strand DNA breaks.
This protein is able to unwind DNA structures may improve the access for DNA repair and replication proteins that are important for preventing the accumulation of abnormal structures.
Title: Biochemical characterization of the WRN-1 RecQ helicase of Caenorhabditis elegans.
WRN-1 acts as a checkpoint protein for DNA damage and replication blockage.
Title: A Werner syndrome protein homolog affects C. elegans development, growth rate, life span and sensitivity to DNA damage by acting at a DNA damage checkpoint.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

F18C5.2 (e-PCR)
- UniSTS:309821

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables 3'-5' DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3'-5' DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables 3'-5' DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables four-way junction helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables isomerase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA geometric change	IEA Inferred from Electronic Annotation more info
involved_in DNA metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA recombination	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA unwinding involved in DNA replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in G-quadruplex DNA unwinding	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular metabolic process	IEA Inferred from Electronic Annotation more info
involved_in determination of adult lifespan	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via homologous recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in nucleic acid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in response to ionizing radiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in chromosome	IBA Inferred from Biological aspect of Ancestor more info
located_in chromosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in site of double-strand break	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: ATP-dependent helicase wrn-1

NP_495324.2

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003280.10 Reference assembly

Range

6554977..6559263

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_062923.8 → NP_495324.2 ATP-dependent helicase wrn-1 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_495324.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q19546

Conserved Domains (7) summary

smart00341
Location:806 → 886

HRDC; Helicase and RNase D C-terminal

COG0514
Location:213 → 882

RecQ; Superfamily II DNA helicase RecQ [Replication, recombination and repair]

cd00079
Location:441 → 549

HELICc; Helicase superfamily c-terminal domain; associated with DEXDc-, DEAD-, and DEAH-box proteins, yeast initiation factor 4A, Ski2p, and Hepatitis C virus NS3 helicases; this domain is found in a wide variety of helicases and helicase related proteins; may ...

pfam00270
Location:232 → 394

DEAD; DEAD/DEAH box helicase

pfam09382
Location:632 → 730

RQC; RQC domain

pfam14493
Location:924 → 1002

HTH_40; Helix-turn-helix domain

pfam16124
Location:554 → 618

RecQ_Zn_bind; RecQ zinc-binding