apn-1 Apurinic-apyrimidinic endonuclease [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: apn-1
Official Full Name: Apurinic-apyrimidinic endonuclease
Primary source: WormBase:WBGene00000151
Locus tag: CELE_T05H10.2
See related: AllianceGenome:WB:WBGene00000151
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables 3'-tyrosyl-DNA phosphodiesterase activity; DNA-(apurinic or apyrimidinic site) endonuclease activity; and double-stranded DNA 3'-5' DNA exonuclease activity. Involved in base-excision repair. Located in nucleus. Is expressed in hermaphrodite gonad and male gonad. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See apn-1 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 3

Sequence:: Chromosome: II; NC_003280.10 (8047564..8049063)

Chromosome II - NC_003280.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003280.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The authors show that APN-1-deficient mutant animals are sensitive to 5-hydroxymethyluracil exposure.
Title: UNG-1 and APN-1 are the major enzymes to efficiently repair 5-hydroxymethyluracil DNA lesions in C. elegans.
Base excision repair endonucleases APN-1 and EXO-3 function in the same pathway as DNA mismatch repair, to elicit DNA-directed toxicity in response to 5-fluorouracil.
Title: Base excision repair AP endonucleases and mismatch repair act together to induce checkpoint-mediated autophagy.
Purified APN-1 displays AP endonuclease activity, 3'-diesterase activity, 3'- to 5'-exonuclease activity and the nucleotide incision repair activity.
Title: Functional characterization of the Caenorhabditis elegans DNA repair enzyme APN-1.
results show that apurinic/apyrimidinic endonuclease 1/EXO-3, p53/CEP-1, and thioredoxin affect each other and that these interactions determine aging as well as neuronal structure and function
Title: Apurinic/apyrimidinic endonuclease 1, p53, and thioredoxin are linked in control of aging in C. elegans.
APN-1 is an important apurinic/apyrimidinc endonuclease for repair of misincorporated uracil.
Title: Caenorhabditis elegans APN-1 plays a vital role in maintaining genome stability.
Apn-1 expresses apurinic/apyrimidinic endonuclease in a Saccharomyces cerevisiae mutant.
Title: Identification of two apurinic/apyrimidinic endonucleases from Caenorhabditis elegans by cross-species complementation.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

T05H10.1 (e-PCR)
- UniSTS:307935

T05H10.2 (e-PCR)
- UniSTS:307936

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables 3'-tyrosyl-DNA phosphodiesterase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-(apurinic or apyrimidinic site) endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-(apurinic or apyrimidinic site) endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables double-stranded DNA 3'-5' DNA exonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphoric diester hydrolase activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphoric diester hydrolase activity	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in base-excision repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in base-excision repair	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Apurinic-apyrimidinic endonuclease

NP_495687.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003280.10 Reference assembly

Range

8047564..8049063

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_063286.8 → NP_495687.1 Apurinic-apyrimidinic endonuclease [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_495687.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q10002

Conserved Domains (2) summary

smart00518
Location:119 → 393

AP2Ec; AP endonuclease family 2

PTZ00372
Location:26 → 393

PTZ00372; endonuclease 4-like protein; Provisional