dpy-18 Prolyl 4-hydroxylase subunit alpha-1 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: dpy-18
Official Full Name: Prolyl 4-hydroxylase subunit alpha-1
Primary source: WormBase:WBGene00001077
Locus tag: CELE_Y47D3B.10
See related: AllianceGenome:WB:WBGene00001077
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables procollagen-proline 4-dioxygenase activity. Involved in several processes, including cuticle development involved in collagen and cuticulin-based cuticle molting cycle; embryo development; and embryonic body morphogenesis. Located in endoplasmic reticulum. Is expressed in hypodermis; muscle cell; neurons; and pharynx. Human ortholog(s) of this gene implicated in myopia. Orthologous to human P4HA1 (prolyl 4-hydroxylase subunit alpha 1) and P4HA2 (prolyl 4-hydroxylase subunit alpha 2). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See dpy-18 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 7

Sequence:: Chromosome: III; NC_003281.10 (11372886..11377980, complement)

Chromosome III - NC_003281.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003281.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

data suggest that DPY-18 regulates ephrin expression to direct axon guidance, a role for P4Hs that may be conserved in higher organisms.
Title: Regulation of axonal midline guidance by prolyl 4-hydroxylation in Caenorhabditis elegans.
although collagen P4Hs, PHY-1/PHY-2/(PDI)(2) mixed tetramer and PHY/PDI dimers, do not act on peptide substrates by a processive mechanism, prevention of hydroxylation at one catalytic site in the tetramer impairs the function of the other catalytic site
Title: Collagen prolyl 4-hydroxylase tetramers and dimers show identical decreases in Km values for peptide substrates with increasing chain length: mutation of one of the two catalytic sites in the tetramer inactivates the enzyme by more than half.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Y47D3B.10 (e-PCR)
- UniSTS:313318

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables L-ascorbic acid binding	IEA Inferred from Electronic Annotation more info
enables dioxygenase activity	IEA Inferred from Electronic Annotation more info
enables iron ion binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	IEA Inferred from Electronic Annotation more info
enables peptidyl-proline 4-dioxygenase activity	IDA Inferred from Direct Assay more info	PubMed
enables procollagen-proline 4-dioxygenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables procollagen-proline 4-dioxygenase activity	IDA Inferred from Direct Assay more info	PubMed
enables procollagen-proline 4-dioxygenase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in IRE1-mediated unfolded protein response	HEP more info	PubMed
involved_in collagen and cuticulin-based cuticle development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cuticle development involved in collagen and cuticulin-based cuticle molting cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in embryo development ending in birth or egg hatching	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in embryonic body morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in macromolecule modification	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of multicellular organism growth	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum lumen	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Prolyl 4-hydroxylase subunit alpha-1

NP_499464.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003281.10 Reference assembly

Range

11372886..11377980 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_067063.6 → NP_499464.1 Prolyl 4-hydroxylase subunit alpha-1 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_499464.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O18153, Q10576

Conserved Domains (2) summary

smart00702
Location:338 → 511

P4Hc; Prolyl 4-hydroxylase alpha subunit homologues

pfam08336
Location:22 → 100

P4Ha_N; Prolyl 4-Hydroxylase alpha-subunit, N-terminal region