tat-1 Phospholipid-transporting ATPase tat-1 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: tat-1
Official Full Name: Phospholipid-transporting ATPase tat-1
Primary source: WormBase:WBGene00013034
Locus tag: CELE_Y49E10.11
See related: AllianceGenome:WB:WBGene00013034
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables ATPase-coupled intramembrane lipid transporter activity. Involved in several processes, including lysosome organization; phospholipid translocation; and regulation of phagocytosis, engulfment. Located in several cellular components, including apical plasma membrane; basolateral plasma membrane; and endosome. Is expressed in several structures, including intestine. Human ortholog(s) of this gene implicated in cerebellar ataxia, impaired intellectual development, and dysequilibrium syndrome 4. Orthologous to several human genes including ATP8A2 (ATPase phospholipid transporting 8A2). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See tat-1 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 20

Sequence:: Chromosome: III; NC_003281.10 (12398901..12421718, complement)

Chromosome III - NC_003281.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003281.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Disruption of phosphatidylserine asymmetry by tat-1 mutation leads to redistribution of positively charged biosensors from plasma membranes to endomembranes.
Title: Inactivation of Caenorhabditis elegans aminopeptidase DNPP-1 restores endocytic sorting and recycling in tat-1 mutants.
TAT-1 is differentially spliced during development and that TAT-1C-specific splicing occurs in the intestine where NUM-1A is known to function.
Title: Caenorhabditis elegans numb inhibits endocytic recycling by binding TAT-1 aminophospholipid translocase.
CHAT-1 and TAT-1 maintain membrane phosphatidylserine asymmetry, thus promoting membrane tubulation and regulating endocytic sorting and recycling.
Title: Endocytic sorting and recycling require membrane phosphatidylserine asymmetry maintained by TAT-1/CHAT-1.
TAT-1 is required at multiple steps of the endolysosomal pathway, at least in part by ensuring proper trafficking of cell-specific effector proteins
Title: The C. elegans P4-ATPase TAT-1 regulates lysosome biogenesis and endocytosis.
study reports that TAT-1 is critical for maintaining cell surface asymmetry of phosphatidylserine (PS); tat-1 appears to function in preventing appearance of PS in the outer leaflet of plasma membrane
Title: Role of C. elegans TAT-1 protein in maintaining plasma membrane phosphatidylserine asymmetry.
Apoptosis, triggered by cell surface phosphatidylserine exposure, requires a functional TAT1 protein.
Title: Aminophospholipid translocase TAT-1 promotes phosphatidylserine exposure during C. elegans apoptosis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Y49E10.11 (e-PCR)
- UniSTS:320406

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATPase-coupled intramembrane lipid transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATPase-coupled intramembrane lipid transporter activity	IEA Inferred from Electronic Annotation more info
enables ATPase-coupled intramembrane lipid transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables magnesium ion binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylserine floppase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lysosome organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of phagocytosis, engulfment	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phospholipid translocation	IBA Inferred from Biological aspect of Ancestor more info
involved_in phospholipid translocation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phospholipid transport	IEA Inferred from Electronic Annotation more info
involved_in phospholipid transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of phagocytosis, engulfment	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in basolateral plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in early endosome	IDA Inferred from Direct Assay more info	PubMed
located_in early endosome membrane	IEA Inferred from Electronic Annotation more info
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in late endosome	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in recycling endosome	IDA Inferred from Direct Assay more info	PubMed
located_in recycling endosome membrane	IEA Inferred from Electronic Annotation more info
is_active_in trans-Golgi network	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Phospholipid-transporting ATPase tat-1

NP_001022894.1

Confirmed by transcript evidence

NP_001255165.1

Confirmed by transcript evidence

NP_001367088.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003281.10 Reference assembly

Range

12398901..12421718 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001027723.3 → NP_001022894.1 Phospholipid-transporting ATPase tat-1 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001022894.1

Status: REVIEWED

UniProtKB/Swiss-Prot

C0P289, Q7JK70, Q9U280

Conserved Domains (5) summary

TIGR01652
Location:28 → 1036

ATPase-Plipid; phospholipid-translocating P-type ATPase, flippase

pfam13246
Location:446 → 545

Cation_ATPase; Cation transport ATPase (P-type)

pfam16209
Location:16 → 79

PhoLip_ATPase_N; Phospholipid-translocating ATPase N-terminal

pfam16212
Location:791 → 1043

PhoLip_ATPase_C; Phospholipid-translocating P-type ATPase C-terminal

cl21460
Location:745 → 789

HAD_like; Haloacid Dehalogenase-like Hydrolases
NM_001268236.3 → NP_001255165.1 Phospholipid-transporting ATPase tat-1 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001255165.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9U280

Conserved Domains (6) summary

TIGR01652
Location:28 → 1036

ATPase-Plipid; phospholipid-translocating P-type ATPase, flippase

pfam00122
Location:85 → 178

E1-E2_ATPase; E1-E2 ATPase

pfam13246
Location:446 → 545

Cation_ATPase; Cation transport ATPase (P-type)

pfam16209
Location:16 → 79

PhoLip_ATPase_N; Phospholipid-translocating ATPase N-terminal

pfam16212
Location:791 → 1043

PhoLip_ATPase_C; Phospholipid-translocating P-type ATPase C-terminal

cl21460
Location:745 → 789

HAD_like; Haloacid Dehalogenase-like Hydrolases
NM_001379952.2 → NP_001367088.1 Phospholipid-transporting ATPase tat-1 [Caenorhabditis elegans]

Status: REVIEWED

Conserved Domains (1) summary

cd02073
Location:30 → 922

P-type_ATPase_APLT_Dnf-like; Aminophospholipid translocases (APLTs), similar to Saccharomyces cerevisiae Dnf1-3p, Drs2p, and human ATP8A2, -10D, -11B, -11C