cyk-4 Rac GTPase-activating protein 1 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: cyk-4
Official Full Name: Rac GTPase-activating protein 1
Primary source: WormBase:WBGene00000875
Locus tag: CELE_K08E3.6
See related: AllianceGenome:WB:WBGene00000875
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables GTPase activator activity. Involved in mitotic cleavage furrow ingression; mitotic spindle midzone assembly; and polar body extrusion after meiotic divisions. Located in mitotic spindle midzone and plasma membrane. Part of centralspindlin complex. Is expressed in several structures, including germ cell; hypodermis; intestine; nervous system; and reproductive system. Human ortholog(s) of this gene implicated in congenital dyserythropoietic anemia. Orthologous to human RACGAP1 (Rac GTPase activating protein 1). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See cyk-4 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 6

Sequence:: Chromosome: III; NC_003281.10 (13768648..13771224, complement)

Chromosome III - NC_003281.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003281.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

CYK4 relaxes the bias in the off-axis motion by MKLP1 kinesin-6.
Title: CYK4 relaxes the bias in the off-axis motion by MKLP1 kinesin-6.
C. elegans CLASP/CLS-2 negatively regulates membrane ingression throughout the oocyte cortex and is required for polar body extrusion.
Title: C. elegans CLASP/CLS-2 negatively regulates membrane ingression throughout the oocyte cortex and is required for polar body extrusion.
Study shows that oocyte production by the syncytial Caenorhabditis elegans germline requires CYK-4 but not ZEN-4, which contrasts with cytokinesis, where both are essential.
Title: CYK-4 functions independently of its centralspindlin partner ZEN-4 to cellularize oocytes in germline syncytia.
Thus, counterintuitively, CYK-4 RhoGAP activity promotes RhoA activation.
Title: The RhoGAP activity of CYK-4/MgcRacGAP functions non-canonically by promoting RhoA activation during cytokinesis.
SPD-1-CYK-4 interaction through the CYK-4 C-terminal tail plays an important role in maintaining the mechanical integrity of the central spindle under tension
Title: Direct interaction between centralspindlin and PRC1 reinforces mechanical resilience of the central spindle.
The membrane-binding C1 domain of CYK-4, a centralspindlin component, promotes furrow initiation in C. elegans embryos and human cells.
Title: Aurora B kinase promotes cytokinesis by inducing centralspindlin oligomers that associate with the plasma membrane.
CYK-4 binding decreases the rate of ZEN-4-mediated microtubule gliding. These results constrain models for the molecular organization of centralspindlin.
Title: Binding of the CYK-4 subunit of the centralspindlin complex induces a large scale conformational change in the kinesin subunit.
CYK-4 subunit functions to recruit actin regulators and the scaffold anillin as well as to regulate RhoA and Rac via its intrinsic GAP domain, ultimately promoting actomyosin contractile ring assembly.
Title: A postmitotic function and distinct localization mechanism for centralspindlin at a stable intercellular bridge.
NOP-1 functions in parallel with CYK-4 to promote RhoA activation.
Title: RhoA activation during polarization and cytokinesis of the early Caenorhabditis elegans embryo is differentially dependent on NOP-1 and CYK-4.
The Rho GTPase-activating protein (GAP) domain of CYK-4 promotes activation of RhoA during cytokinesis.
Title: The RhoGAP domain of CYK-4 has an essential role in RhoA activation.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

K08E3.6 (e-PCR)
- UniSTS:312689

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables GTPase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Rho protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic cleavage furrow ingression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic cytokinesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic sister chromatid segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle midzone assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic spindle midzone assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in polar body extrusion after meiotic divisions	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of centralspindlin complex	IBA Inferred from Biological aspect of Ancestor more info
part_of centralspindlin complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in cleavage furrow	IBA Inferred from Biological aspect of Ancestor more info
is_active_in midbody	IBA Inferred from Biological aspect of Ancestor more info
located_in mitotic spindle midzone	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in spindle midzone	IBA Inferred from Biological aspect of Ancestor more info
located_in spindle midzone	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Rac GTPase-activating protein 1

NP_499845.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003281.10 Reference assembly

Range

13768648..13771224 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_067444.9 → NP_499845.1 Rac GTPase-activating protein 1 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_499845.1

Status: REVIEWED

UniProtKB/TrEMBL

Q9XUS9

Conserved Domains (3) summary

cd04382
Location:418 → 615

RhoGAP_MgcRacGAP; RhoGAP_MgcRacGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in MgcRacGAP proteins. MgcRacGAP plays an important dual role in cytokinesis: i) it is part of centralspindlin-complex, together with the mitotic kinesin ...

pfam17023
Location:33 → 184

DUF5098; Domain of unknown function (DUF5098)

cl00040
Location:352 → 402

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...