pxf-1 Rap guanine nucleotide exchange factor;Rap guanine nucleotide exchange factor 2 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: pxf-1
Official Full Name: Rap guanine nucleotide exchange factor;Rap guanine nucleotide exchange factor 2
Primary source: WormBase:WBGene00004254
Locus tag: CELE_T14G10.2
See related: AllianceGenome:WB:WBGene00004254
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables guanyl-nucleotide exchange factor activity. Involved in collagen and cuticulin-based cuticle development; epidermis development; and molting cycle. Located in collagen and cuticulin-based cuticle extracellular matrix. Is expressed in several structures, including cholinergic neurons; hermaphrodite somatic gonadal cell; hypodermis; intestine; and somatic nervous system. Human ortholog(s) of this gene implicated in familial adult myoclonic epilepsy 7. Orthologous to human RAPGEF2 (Rap guanine nucleotide exchange factor 2) and RAPGEF6 (Rap guanine nucleotide exchange factor 6). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See pxf-1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 17

Sequence:: Chromosome: IV; NC_003282.8 (10164288..10174341, complement)

Chromosome IV - NC_003282.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003282.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

We conclude that RAP-1 is a positive regulator that promotes Ras-dependent inductive fate decisions. We hypothesize that PXF-1 activation of RAP-1 serves as a minor parallel input into the major LET-60/Ras signal through LIN-45/Raf
Title: Ras-Dependent Cell Fate Decisions Are Reinforced by the RAP-1 Small GTPase in Caenorhabditiselegans.
the pxf-1/rap pathway in C. elegans is required for maintenance of epithelial integrity, in which it probably functions in polarized secretion
Title: Requirement of the Caenorhabditis elegans RapGEF pxf-1 and rap-1 for epithelial integrity.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

T14G10.2 (e-PCR)
- UniSTS:308031

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables GTPase activator activity	IEA Inferred from Electronic Annotation more info
enables guanyl-nucleotide exchange factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables guanyl-nucleotide exchange factor activity	IDA Inferred from Direct Assay more info	PubMed
enables guanyl-nucleotide exchange factor activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Ras protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in collagen and cuticulin-based cuticle development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in epidermis development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in molting cycle, collagen and cuticulin-based cuticle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
involved_in small GTPase-mediated signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in apical plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in collagen and cuticulin-based cuticle extracellular matrix	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: Rap guanine nucleotide exchange factor;Rap guanine nucleotide exchange factor 2

NP_001023389.1

Confirmed by transcript evidence

NP_001023390.1

Partially confirmed by transcript evidence

NP_001255465.1

Confirmed by transcript evidence

NP_001255466.1

Confirmed by transcript evidence

NP_001255467.1

Confirmed by transcript evidence

NP_001367298.1

Confirmed by transcript evidence

NP_001367299.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003282.8 Reference assembly

Range

10164288..10174341 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001028218.6 → NP_001023389.1 Rap guanine nucleotide exchange factor [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001023389.1

Status: REVIEWED

UniProtKB/Swiss-Prot

G5EBY1, G5EDB9, G5EEX2

Conserved Domains (6) summary

cd01785
Location:782 → 867

PDZ_GEF_RA; Ubiquitin-like domain of PDZ_GEF_RA

smart00100
Location:348 → 458

cNMP; Cyclic nucleotide-monophosphate binding domain

smart00229
Location:479 → 592

RasGEFN; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif

cd00038
Location:45 → 131

CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...

cd00155
Location:890 → 1118

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of the Ras superfamily function as molecular switches in fundamental events such as signal transduction, cytoskeleton dynamics and intracellular trafficking. ...

cd00992
Location:595 → 662

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
NM_001028219.3 → NP_001023390.1 Rap guanine nucleotide exchange factor [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001023390.1

Status: REVIEWED

UniProtKB/Swiss-Prot

G5EDB9

Conserved Domains (6) summary

cd01785
Location:782 → 867

PDZ_GEF_RA; Ubiquitin-like domain of PDZ_GEF_RA

smart00100
Location:348 → 458

cNMP; Cyclic nucleotide-monophosphate binding domain

smart00229
Location:479 → 592

RasGEFN; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif

cd00038
Location:45 → 131

CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...

cd00155
Location:890 → 1118

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of the Ras superfamily function as molecular switches in fundamental events such as signal transduction, cytoskeleton dynamics and intracellular trafficking. ...

cd00992
Location:595 → 662

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
NM_001268536.3 → NP_001255465.1 Rap guanine nucleotide exchange factor [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001255465.1

Status: REVIEWED

UniProtKB/Swiss-Prot

G5EDB9

Conserved Domains (5) summary

cd01785
Location:659 → 744

PDZ_GEF_RA; Ubiquitin-like domain of PDZ_GEF_RA

smart00100
Location:225 → 335

cNMP; Cyclic nucleotide-monophosphate binding domain

smart00229
Location:356 → 469

RasGEFN; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif

cd00155
Location:767 → 995

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of the Ras superfamily function as molecular switches in fundamental events such as signal transduction, cytoskeleton dynamics and intracellular trafficking. ...

cd00992
Location:472 → 539

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
NM_001268537.3 → NP_001255466.1 Rap guanine nucleotide exchange factor 2 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001255466.1

Status: REVIEWED

UniProtKB/TrEMBL

G5EEE0

Conserved Domains (5) summary

cd01785
Location:617 → 702

PDZ_GEF_RA; Ubiquitin-like domain of PDZ_GEF_RA

smart00100
Location:183 → 293

cNMP; Cyclic nucleotide-monophosphate binding domain

smart00229
Location:314 → 427

RasGEFN; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif

cd00155
Location:725 → 953

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of the Ras superfamily function as molecular switches in fundamental events such as signal transduction, cytoskeleton dynamics and intracellular trafficking. ...

cd00992
Location:430 → 497

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
NM_001268538.3 → NP_001255467.1 Rap guanine nucleotide exchange factor [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001255467.1

Status: REVIEWED

UniProtKB/TrEMBL

F5GUA2
NM_001380435.1 → NP_001367298.1 Rap guanine nucleotide exchange factor 2 [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/TrEMBL

G5ECE2

Conserved Domains (5) summary

smart00100
Location:225 → 335

cNMP; Cyclic nucleotide-monophosphate binding domain

smart00229
Location:356 → 469

RasGEFN; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif

cd00155
Location:767 → 995

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of the Ras superfamily function as molecular switches in fundamental events such as signal transduction, cytoskeleton dynamics and intracellular trafficking. ...

cd00992
Location:472 → 539

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

cd01785
Location:661 → 744

RA_PDZ-GEF1; Ras-associating (RA) domain found in PDZ domain-containing guanine nucleotide exchange factor 1 (PDZ-GEF1) and similar proteins
NM_001380436.1 → NP_001367299.1 Rap guanine nucleotide exchange factor 2 [Caenorhabditis elegans]

Status: REVIEWED

UniProtKB/TrEMBL

G5ECB8

Conserved Domains (5) summary

smart00100
Location:183 → 293

cNMP; Cyclic nucleotide-monophosphate binding domain

smart00229
Location:314 → 427

RasGEFN; Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif

cd00155
Location:725 → 953

RasGEF; Guanine nucleotide exchange factor for Ras-like small GTPases. Small GTP-binding proteins of the Ras superfamily function as molecular switches in fundamental events such as signal transduction, cytoskeleton dynamics and intracellular trafficking. ...

cd00992
Location:430 → 497

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

cd01785
Location:619 → 702

RA_PDZ-GEF1; Ras-associating (RA) domain found in PDZ domain-containing guanine nucleotide exchange factor 1 (PDZ-GEF1) and similar proteins