U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Pcp2 Purkinje cell protein 2 (L7) [ Mus musculus (house mouse) ]

Gene ID: 18545, updated on 2-Nov-2024

Summary

Go to the top of the page Help
Official Symbol
Pcp2provided by MGI
Official Full Name
Purkinje cell protein 2 (L7)provided by MGI
Primary source
MGI:MGI:97508
See related
Ensembl:ENSMUSG00000004630 AllianceGenome:MGI:97508
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
L7; Pcp-2
Summary
Enables guanyl-nucleotide exchange factor activity. Acts upstream of or within G protein-coupled opsin signaling pathway. Is expressed in central nervous system and retina. Orthologous to human PCP2 (Purkinje cell protein 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Restricted expression toward cerebellum adult (RPKM 249.4) See more
Orthologs
human all
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
See Pcp2 in Genome Data Viewer
Location:
8 A1.1; 8 1.92 cM
Exon count:
7
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 8 NC_000074.7 (3673369..3675547, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 8 NC_000074.6 (3623369..3625547, complement)

Chromosome 8 - NC_000074.7Genomic Context describing neighboring genes Neighboring gene calmodulin regulated spectrin-associated protein family, member 3 Neighboring gene STARR-seq mESC enhancer starr_20594 Neighboring gene XPA binding protein 2 Neighboring gene STARR-positive B cell enhancer ABC_E3766 Neighboring gene PET100 homolog Neighboring gene syntaxin binding protein 2 Neighboring gene resistin

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help
  • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Purkinje cell protein-2 regulatory regions and transgene expression in cerebellar compartments. Vandaele S, et al. Genes Dev, 1991 Jul. PMID 2065970
  2. Association and colocalization of G protein alpha subunits and Purkinje cell protein 2 (Pcp2) in mammalian cerebellum. Redd KJ, et al. J Neurosci Res, 2002 Dec 1. PMID 12424730
  3. Sensorimotor enhancement in mouse mutants lacking the Purkinje cell-specific Gi/o modulator, Pcp2(L7). Iscru E, et al. Mol Cell Neurosci, 2009 Jan. PMID 18930827, Free PMC Article
  4. Phenotypic analysis of mice lacking the highly abundant Purkinje cell- and bipolar neuron-specific PCP2 protein. Mohn AR, et al. Mol Cell Neurosci, 1997 Jan. PMID 9204480
  5. Sex-dependent behavioral functions of the Purkinje cell-specific Gαi/o binding protein, Pcp2(L7). Walton JC, et al. Cerebellum, 2012 Dec. PMID 22528962, Free PMC Article

See all (76) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. New observations from this study include altered expression of Pcp2 and Limp2 in Npc1 mutant mice relative to control, with Pcp2 exhibiting multiple isoforms and specific to the cerebella. This study provides valuable insight into pathways altered in the late-stage pathophysiology of NPC1
    Title: Quantitative, Label-Free Proteomics in the Symptomatic Niemann-Pick, Type C1 Mouse Model Using Standard Flow Liquid Chromatography and Thermal Focusing Electrospray Ionization.
  2. A novel Pcp2 transcript is particular to mouse cerebellar Purkinje cells and retinal bipolar neurons.
    Title: Expression of a novel splicing variant of Pcp2 in closely related laboratory rodents.
  3. Study describes alternative splicing of the pcp2/L7 gene; biochemical properties of short and long form differ
    Title: Developmental upregulation of an alternative form of pcp2 with reduced GDI activity.
  4. The results of this study showed that L7-PP2B mice, which suffer from impaired intrinsic plasticity and synaptic potentiation of their Purkinje cells.
    Title: Cerebellar potentiation and learning a whisker-based object localization task with a time response window.
  5. This study characterized the Cre-positive retinal ganglion cells in a PCP2 (Purkinje cell protein 2)-cre mouse line. We found that all of the Cre-positive retinal ganglion cells were bistratified retinal ganglion cells.
    Title: Characterization of multiple bistratified retinal ganglion cells in a purkinje cell protein 2-Cre transgenic mouse line.
  6. both L7 genotype and sex contribute to both acquisition and extinction of fear conditioning is affected in Pcp2(L7) mutants
    Title: Sex-dependent behavioral functions of the Purkinje cell-specific Gαi/o binding protein, Pcp2(L7).
  7. Results suggest that the Pcp2(L7) protein acts as a sensorimotor damper that modulates time- and sense-dependent changes in motor responses.
    Title: Sensorimotor enhancement in mouse mutants lacking the Purkinje cell-specific Gi/o modulator, Pcp2(L7).
  8. We conclude that PCP2 facilitates cation channels closure in the dark, shortens the rise time of the light response directly, and accelerates the decay time indirectly via the inhibitory network.
    Title: Retinal ON bipolar cells express a new PCP2 splice variant that accelerates the light response.
  9. Two mRNA forms predict two forms of the L7/Pcp-2 protein, which are both highly conserved across species.In mouse both mRNA forms are detectable in dendrites but their relative proportions change during development.
    Title: Conservation of the developmentally regulated dendritic localization of a Purkinje cell-specific mRNA that encodes a G-protein modulator: comparison of rodent and human Pcp2(L7) gene structure and expression.
  10. effect on expression of cre recombinase
    Title: L7/Pcp-2-specific expression of Cre recombinase using knock-in approach.
Submit: New GeneRIF Correction See all GeneRIFs (13)

Variation

Go to the top of the page Help

Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (1) 
  • Targeted (4)  1 citation

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables guanyl-nucleotide exchange factor activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables guanyl-nucleotide exchange factor activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables guanyl-nucleotide exchange factor activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within G protein-coupled opsin signaling pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 

General protein information

Go to the top of the page Help
Preferred Names
Purkinje cell protein 2
Names
Purkinje cell-specific protein L7
protein PCD-5
retinal Purkinje cell protein 2

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001129803.2 → NP_001123275.1  Purkinje cell protein 2 isoform 1

    See identical proteins and their annotated locations for NP_001123275.1

    Status: VALIDATED

    Source sequence(s)
    AC170806
    Consensus CDS
    CCDS52471.1
    UniProtKB/TrEMBL
    A8IK50
    Related
    ENSMUSP00000121079.2, ENSMUST00000136105.8
    Conserved Domains (2) summary
    smart00390
    Location:63 → 85
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases
    pfam02188
    Location:24 → 45
    GoLoco; GoLoco motif

  2. NM_001129804.2 → NP_001123276.1  Purkinje cell protein 2 isoform 2

    See identical proteins and their annotated locations for NP_001123276.1

    Status: VALIDATED

    Source sequence(s)
    AC170806
    Consensus CDS
    CCDS52470.1
    UniProtKB/Swiss-Prot
    P12660, P22941, Q3TV53
    Related
    ENSMUSP00000122902.2, ENSMUST00000133459.8
    Conserved Domains (2) summary
    smart00390
    Location:47 → 69
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases
    pfam02188
    Location:8 → 29
    GoLoco; GoLoco motif

  3. NM_001372442.1 → NP_001359371.1  Purkinje cell protein 2 isoform 4

    Status: VALIDATED

    Source sequence(s)
    AC170806
    Conserved Domains (2) summary
    smart00390
    Location:48 → 70
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases
    pfam02188
    Location:9 → 29
    GoLoco; GoLoco motif

  4. NM_001372443.1 → NP_001359372.1  Purkinje cell protein 2 isoform 5

    Status: VALIDATED

    Source sequence(s)
    AC170806
    UniProtKB/TrEMBL
    A0A140LHG1, A0A140LI57
    Related
    ENSMUSP00000146399.2, ENSMUST00000144977.2
    Conserved Domains (2) summary
    smart00390
    Location:63 → 85
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases
    pfam02188
    Location:25 → 45
    GoLoco; GoLoco motif

  5. NM_001372444.1 → NP_001359373.1  Purkinje cell protein 2 isoform 6

    Status: VALIDATED

    Source sequence(s)
    AC170806
    Conserved Domains (1) summary
    smart00390
    Location:27 → 49
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

  6. NM_001372445.1 → NP_001359374.1  Purkinje cell protein 2 isoform 6

    Status: VALIDATED

    Source sequence(s)
    AC170806
    Conserved Domains (1) summary
    smart00390
    Location:27 → 49
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

  7. NM_001372446.1 → NP_001359375.1  Purkinje cell protein 2 isoform 6

    Status: VALIDATED

    Source sequence(s)
    AC170806
    Conserved Domains (1) summary
    smart00390
    Location:27 → 49
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

  8. NM_001372447.1 → NP_001359376.1  Purkinje cell protein 2 isoform 6

    Status: VALIDATED

    Source sequence(s)
    AC170806
    Conserved Domains (1) summary
    smart00390
    Location:27 → 49
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

  9. NM_001372448.1 → NP_001359377.1  Purkinje cell protein 2 isoform 6

    Status: VALIDATED

    Source sequence(s)
    AC170806
    Conserved Domains (1) summary
    smart00390
    Location:27 → 49
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

  10. NM_001372449.1 → NP_001359378.1  Purkinje cell protein 2 isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC170806
    Consensus CDS
    CCDS22066.1
    UniProtKB/TrEMBL
    A0A0R4IZX8
    Conserved Domains (1) summary
    smart00390
    Location:26 → 48
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

  11. NM_001372450.1 → NP_001359379.1  Purkinje cell protein 2 isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC170806
    UniProtKB/TrEMBL
    A0A0R4IZX8
    Related
    ENSMUSP00000121403.2, ENSMUST00000142431.8
    Conserved Domains (1) summary
    smart00390
    Location:26 → 48
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

  12. NM_001372451.1 → NP_001359380.1  Purkinje cell protein 2 isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC170806
    UniProtKB/TrEMBL
    A0A0R4IZX8
    Conserved Domains (1) summary
    smart00390
    Location:26 → 48
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

  13. NM_001372452.1 → NP_001359381.1  Purkinje cell protein 2 isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC170806
    UniProtKB/TrEMBL
    A0A0R4IZX8
    Conserved Domains (1) summary
    smart00390
    Location:26 → 48
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

  14. NM_001372453.1 → NP_001359382.1  Purkinje cell protein 2 isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC170806
    UniProtKB/TrEMBL
    A0A0R4IZX8
    Conserved Domains (1) summary
    smart00390
    Location:26 → 48
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

  15. NM_001372454.1 → NP_001359383.1  Purkinje cell protein 2 isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC170806
    UniProtKB/TrEMBL
    A0A0R4IZX8
    Conserved Domains (1) summary
    smart00390
    Location:26 → 48
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

  16. NM_001372455.1 → NP_001359384.1  Purkinje cell protein 2 isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC170806
    UniProtKB/TrEMBL
    A0A0R4IZX8
    Conserved Domains (1) summary
    smart00390
    Location:26 → 48
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

  17. NM_001372456.1 → NP_001359385.1  Purkinje cell protein 2 isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC170806
    UniProtKB/TrEMBL
    A0A0R4IZX8
    Conserved Domains (1) summary
    smart00390
    Location:26 → 48
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

  18. NM_008790.3 → NP_032816.1  Purkinje cell protein 2 isoform 3

    See identical proteins and their annotated locations for NP_032816.1

    Status: VALIDATED

    Source sequence(s)
    AC170806
    Consensus CDS
    CCDS22066.1
    UniProtKB/TrEMBL
    A0A0R4IZX8
    Related
    ENSMUSP00000004749.7, ENSMUST00000004749.7
    Conserved Domains (1) summary
    smart00390
    Location:26 → 48
    GoLoco; LGN motif, putative GEFs specific for G-alpha GTPases

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000074.7 Reference GRCm39 C57BL/6J

    Range
    3673369..3675547 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P12660.2 GenPept UniProtKB/Swiss-Prot:P12660

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Chemical Information
Molecular Biology Databases
Research Materials
Tools