Cntn2 contactin 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cntn2provided by MGI
Official Full Name: contactin 2provided by MGI
Primary source: MGI:MGI:104518
See related: Ensembl:ENSMUSG00000053024 AllianceGenome:MGI:104518
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Tax; TAG1; TAG-1; D130012K04Rik
Summary: This gene encodes a member of the contactin family of proteins, part of the immunoglobulin superfamily of cell adhesion molecules. The encoded glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein plays a role in the proliferation, migration, and axon guidance of neurons of the developing cerebellum. Mice lacking a functional copy of this gene exhibit epileptic seizures and elevated expression of A1 adenosine receptors. [provided by RefSeq, Sep 2016]
Expression: Biased expression in cerebellum adult (RPKM 27.9), CNS E14 (RPKM 19.9) and 5 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1 E4; 1 57.42 cM

Exon count:: 26

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (132437163..132470989, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (132509425..132543251, complement)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Using the Allen gene expression atlas of the adult mouse brain to gain further insight into the physiological significance of TAG-1/Contactin-2.
Title: Using the Allen gene expression atlas of the adult mouse brain to gain further insight into the physiological significance of TAG-1/Contactin-2.
TAG-1 Multifunctionality Coordinates Neuronal Migration, Axon Guidance, and Fasciculation.
Title: TAG-1 Multifunctionality Coordinates Neuronal Migration, Axon Guidance, and Fasciculation.
we identify an additional variant (N407S) with a dominant-negative effect on axon growth although it is well-localized at the membrane and properly binds to Contactin2
Title: Genetic variants in autism-related CNTNAP2 impair axonal growth of cortical neurons.
During development, CNTN2 absence can transiently affect the expression levels of myelin and myelin-regulating genes, and results in impaired oligodendrocyte branching and hypomyelination of fiber tracts. During de- and remyelination, Cntn2 absence did not affect oligodendrocyte survival or the extent of remyelination.
Title: The function of contactin-2/TAG-1 in oligodendrocytes in health and demyelinating pathology.
We further demonstrate that Cre expression initiates after birth with preservation of native Cntn2 protein. Finally, we show that Cntn23'UTR-IRES-Cre-EGFP/+ mice maintain normal cardiac mechanical and electrical function.
Title: Phenotypically silent Cre recombination within the postnatal ventricular conduction system.
Tag1 is crucial for the olfactory circuit formation in mice.
Title: Tag1 deficiency results in olfactory dysfunction through impaired migration of mitral cells.
Interaction proteomics revealed the interactors of Caspr2, including CNTN2, KCNAs, members of the ADAM family (ADAM22, ADAM23 and ADAM11), members of LGI family and MAGUKs (DLGs and MPPs).
Title: Interaction proteomics of canonical Caspr2 (CNTNAP2) reveals the presence of two Caspr2 isoforms with overlapping interactomes.
Our results clearly demonstrate that mouse and human contactin-2 are physiological substrates for BACE1
Title: BACE1 activity regulates cell surface contactin-2 levels.
the TAG-1-mediated cell-to-cell interaction between the unpolarized multipolar cells and the pioneering axons regulates the polarization of multipolar cells partly through Lyn kinase and Rac1
Title: Pioneering axons regulate neuronal polarization in the developing cerebral cortex.
This study demonistrated that knockdown of the cell-surface molecule TAG-1 resulted in retraction of neocortical progenitors' basal processes.
Title: TAG-1-assisted progenitor elongation streamlines nuclear migration to optimize subapical crowding.

Submit: New GeneRIF Correction See all GeneRIFs (25)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (5)
Targeted (7) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Cntn2 (e-PCR), detects polymorphism
- UniSTS:531332

RH142422 (e-PCR)
- UniSTS:231669

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables carbohydrate binding	IDA Inferred from Direct Assay more info	PubMed
enables cell adhesion mediator activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables cell-cell adhesion mediator activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of G protein-coupled adenosine receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within adult walking behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axon guidance	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within axon guidance	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within axonal fasciculation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axonogenesis	ISO Inferred from Sequence Orthology more info
involved_in brain development	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in cell-cell adhesion	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell-matrix adhesion	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within central nervous system myelination	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cerebral cortex GABAergic interneuron migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in clustering of voltage-gated potassium channels	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within establishment of localization in cell	IDA Inferred from Direct Assay more info	PubMed
involved_in establishment of protein localization to juxtaparanode region of axon	IDA Inferred from Direct Assay more info	PubMed
involved_in fat cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within learning	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within microtubule cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of neuron differentiation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of neuron differentiation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within neuron migration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neuron projection development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within positive regulation of adenosine receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of protein processing	IDA Inferred from Direct Assay more info	PubMed
involved_in presynaptic membrane organization	ISO Inferred from Sequence Orthology more info
involved_in presynaptic membrane organization	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within protein localization to juxtaparanode region of axon	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within protein localization to juxtaparanode region of axon	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to juxtaparanode region of axon	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of protein processing	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within receptor internalization	IDA Inferred from Direct Assay more info	PubMed
involved_in reduction of food intake in response to dietary excess	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of astrocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of axon diameter	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of neuronal synaptic plasticity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to dietary excess	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in axon	IBA Inferred from Biological aspect of Ancestor more info
located_in axon	IDA Inferred from Direct Assay more info	PubMed
is_active_in axon initial segment	IDA Inferred from Direct Assay more info	PubMed
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
located_in juxtaparanode region of axon	IDA Inferred from Direct Assay more info	PubMed
located_in myelin sheath	IDA Inferred from Direct Assay more info	PubMed
located_in neuron projection	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in node of Ranvier	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in postsynaptic membrane	IEA Inferred from Electronic Annotation more info
is_active_in postsynaptic membrane	ISO Inferred from Sequence Orthology more info
located_in side of membrane	IEA Inferred from Electronic Annotation more info
located_in synapse	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: contactin-2

Names: TAX-1; axonal glycoprotein TAG-1; axonin; axonin-1; transient axonal glycoprotein 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_177129.5 → NP_796103.2 contactin-2 precursor

See identical proteins and their annotated locations for NP_796103.2

Status: REVIEWED

Source sequence(s)

AK083808, BC066106, BC079585, CJ108593

Consensus CDS

CCDS15288.1

UniProtKB/Swiss-Prot

Q61330, Q6NZJ4, Q7TSJ5

UniProtKB/TrEMBL

Q3UH39

Related

ENSMUSP00000083707.5, ENSMUST00000086521.11

Conserved Domains (5) summary

cd05727
Location:133 → 230

Ig2_Contactin-2-like; Second Ig domain of the neural cell adhesion molecule contactin-2 and similar proteins

cd05728
Location:330 → 414

Ig4_Contactin-2-like; Fourth Ig domain of the neural cell adhesion molecule contactin-2 and similar proteins

cd00063
Location:817 → 909

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd04969
Location:435 → 507

Ig5_Contactin; Fifth immunoglobulin (Ig) domain of contactin

cl11960
Location:38 → 131

Ig; Immunoglobulin domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000067.7 Reference GRCm39 C57BL/6J

Range

132437163..132470989 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006529362.2 → XP_006529425.1 contactin-2 isoform X1

See identical proteins and their annotated locations for XP_006529425.1

UniProtKB/Swiss-Prot

Q61330, Q6NZJ4, Q7TSJ5

UniProtKB/TrEMBL

Q3UH39

Conserved Domains (5) summary

cd05727
Location:133 → 230

Ig2_Contactin-2-like; Second Ig domain of the neural cell adhesion molecule contactin-2 and similar proteins

cd05728
Location:330 → 414

Ig4_Contactin-2-like; Fourth Ig domain of the neural cell adhesion molecule contactin-2 and similar proteins

cd00063
Location:817 → 909

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd04969
Location:435 → 507

Ig5_Contactin; Fifth immunoglobulin (Ig) domain of contactin

cl11960
Location:38 → 131

Ig; Immunoglobulin domain

RNA

XR_878318.3 RNA Sequence

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_011531.3: Suppressed sequence

Description

NM_011531.3: This RefSeq was permanently suppressed because it is a nonsense-mediated decay (NMD) candidate.