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MARCHF8 membrane associated ring-CH-type finger 8 [ Homo sapiens (human) ]

Gene ID: 220972, updated on 3-Nov-2024

Summary

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Official Symbol
MARCHF8provided by HGNC
Official Full Name
membrane associated ring-CH-type finger 8provided by HGNC
Primary source
HGNC:HGNC:23356
See related
Ensembl:ENSG00000165406 MIM:613335; AllianceGenome:HGNC:23356
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
MIR; CMIR; c-MIR; MARCH8; RNF178; MARCH-VIII
Summary
MARCH8 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH8 induces the internalization of several membrane glycoproteins (Goto et al., 2003 [PubMed 12582153]; Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010]
Expression
Ubiquitous expression in testis (RPKM 18.4), bone marrow (RPKM 12.0) and 25 other tissues See more
Orthologs
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Genomic context

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See MARCHF8 in Genome Data Viewer
Location:
10q11.21-q11.22
Exon count:
15
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 10 NC_000010.11 (45454585..45594907, complement)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 10 NC_060934.1 (46335573..46475913, complement)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 10 NC_000010.10 (45950033..46090355, complement)

Chromosome 10 - NC_000010.11Genomic Context describing neighboring genes Neighboring gene olfactory receptor family 13 subfamily A member 1 Neighboring gene ReSE screen-validated silencer GRCh37_chr10:45839254-45839455 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr10:45869432-45870042 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr10:45870043-45870651 Neighboring gene arachidonate 5-lipoxygenase Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr10:45914387-45915080 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr10:45915081-45915772 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr10:45915801-45916302 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr10:45921315-45921815 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr10:45923135-45923798 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr10:45928754-45929292 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr10:45928214-45928753 Neighboring gene MED14-independent group 3 enhancer GRCh37_chr10:45933181-45934380 Neighboring gene ReSE screen-validated silencer GRCh37_chr10:45937030-45937261 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr10:45937618-45938388 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr10:45938389-45939157 Neighboring gene uncharacterized LOC102724323 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr10:45958734-45959933 Neighboring gene uncharacterized LOC105378286 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 3321 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr10:45970297-45970798 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr10:45970799-45971298 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr10:45998823-45999323 Neighboring gene VISTA enhancer hs2065 Neighboring gene OCT4-NANOG-H3K27ac hESC enhancer GRCh37_chr10:46070300-46070960 Neighboring gene H3K27ac hESC enhancer GRCh37_chr10:46086550-46087050 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 2344 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 2345 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 2346 Neighboring gene zinc finger AN1-type containing 4 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 3322 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 2347 Neighboring gene ArfGAP with GTPase domain, ankyrin repeat and PH domain 10, pseudogene

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. The Salmonella Effector SteD Mediates MARCH8-Dependent Ubiquitination of MHC II Molecules and Inhibits T Cell Activation. Bayer-Santos E, et al. Cell Host Microbe, 2016 Nov 9. PMID 27832589, Free PMC Article
  2. MARCH8 Inhibits Ebola Virus Glycoprotein, Human Immunodeficiency Virus Type 1 Envelope Glycoprotein, and Avian Influenza Virus H5N1 Hemagglutinin Maturation. Yu C, et al. mBio, 2020 Sep 15. PMID 32934085, Free PMC Article
  3. MARCH8 inhibits viral infection by two different mechanisms. Zhang Y, et al. Elife, 2020 Aug 11. PMID 32778221, Free PMC Article
  4. Sh-MARCH8 Inhibits Tumorigenesis via PI3K Pathway in Gastric Cancer. Yin J, et al. Cell Physiol Biochem, 2018. PMID 30138931
  5. MARCH8 Restricts Influenza A Virus Infectivity but Does Not Downregulate Viral Glycoprotein Expression at the Surface of Infected Cells. Villalón-Letelier F, et al. mBio, 2021 Oct 26. PMID 34517760, Free PMC Article

See all (62) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. MARCH8 inhibits pseudorabies virus replication by trapping the viral cell-to-cell fusion complex in the trans-Golgi network.
    Title: MARCH8 inhibits pseudorabies virus replication by trapping the viral cell-to-cell fusion complex in the trans-Golgi network.
  2. The membrane-associated ubiquitin ligase MARCHF8 stabilizes the human papillomavirus oncoprotein E7 by degrading CUL1 and UBE2L3 in head and neck cancer.
    Title: The membrane-associated ubiquitin ligase MARCHF8 stabilizes the human papillomavirus oncoprotein E7 by degrading CUL1 and UBE2L3 in head and neck cancer.
  3. Human MARCH1, 2, and 8 block Ebola virus envelope glycoprotein cleavage via targeting furin P domain.
    Title: Human MARCH1, 2, and 8 block Ebola virus envelope glycoprotein cleavage via targeting furin P domain.
  4. EGR1 functions as a new host restriction factor for SARS-CoV-2 to inhibit virus replication through the E3 ubiquitin ligase MARCH8.
    Title: EGR1 functions as a new host restriction factor for SARS-CoV-2 to inhibit virus replication through the E3 ubiquitin ligase MARCH8.
  5. E3 Ubiquitin Ligase MARCH8 Promotes Pancreatic Cancer Growth and Metastasis by Activating STAT3 via Degradation of PTPN4.
    Title: E3 Ubiquitin Ligase MARCH8 Promotes Pancreatic Cancer Growth and Metastasis by Activating STAT3 via Degradation of PTPN4.
  6. Ubiquitin ligase MARCH8 promotes the malignant progression of hepatocellular carcinoma through PTEN ubiquitination and degradation.
    Title: Ubiquitin ligase MARCH8 promotes the malignant progression of hepatocellular carcinoma through PTEN ubiquitination and degradation.
  7. HPV upregulates MARCHF8 ubiquitin ligase and inhibits apoptosis by degrading the death receptors in head and neck cancer.
    Title: HPV upregulates MARCHF8 ubiquitin ligase and inhibits apoptosis by degrading the death receptors in head and neck cancer.
  8. Membrane-associated RING-CH protein (MARCH8) is a novel glycolysis repressor targeted by miR-32 in colorectal cancer.
    Title: Membrane-associated RING-CH protein (MARCH8) is a novel glycolysis repressor targeted by miR-32 in colorectal cancer.
  9. Membrane-Associated RING-CH 8 Functions as a Novel PD-L1 E3 Ligase to Mediate PD-L1 Degradation Induced by EGFR Inhibitors.
    Title: Membrane-Associated RING-CH 8 Functions as a Novel PD-L1 E3 Ligase to Mediate PD-L1 Degradation Induced by EGFR Inhibitors.
  10. MARCH8 Restricts Influenza A Virus Infectivity but Does Not Downregulate Viral Glycoprotein Expression at the Surface of Infected Cells.
    Title: MARCH8 Restricts Influenza A Virus Infectivity but Does Not Downregulate Viral Glycoprotein Expression at the Surface of Infected Cells.
Submit: New GeneRIF Correction See all GeneRIFs (32)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
A meta-analysis of 87,040 individuals identifies 23 new susceptibility loci for prostate cancer.
EBI GWAS Catalog
An atlas of genetic influences on human blood metabolites.
EBI GWAS Catalog
Discovery and refinement of loci associated with lipid levels.
EBI GWAS Catalog
Genome-wide association study of hematological and biochemical traits in a Japanese population.
EBI GWAS Catalog
Multiple loci influence erythrocyte phenotypes in the CHARGE Consortium.
EBI GWAS Catalog
Seventy-five genetic loci influencing the human red blood cell.
EBI GWAS Catalog

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

HIV-1 interactions

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Replication interactions

Interaction Pubs
HIV-1 replication is inhibited by MARCH8 expression in monocyte derived macrophages as replication is recovered through MARCH8 knockdown by shRNA PubMed
HIV-1 replication is inhibited by MARCH8 expression in 293T or MAGIC5 cells PubMed
Knockdown of membrane-associated ring finger (C3HC4) 8, E3 ubiquitin protein ligase (MARCH8) by siRNA enhances the early stages of HIV-1 replication in HeLa-CD4 cells infected with viral pseudotypes HIV89.6R and HIV8.2N PubMed

Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env HIV-1 Env (gp120) binds MARCH8 leading to Env (gp120) removal from the surface of cells PubMed
env HIV-1 Env (gp120) incorporation into virions is inhibited by MARCH8 expression PubMed
env HIV-1 Env (gp120) binds MARCH8 leading to Env (gp120) downregulation from the surface of cells PubMed
Pr55(Gag) gag Expression of MARCH-8 inhibits HLA-DR-mediated enhancement of mature Gag products internalization by downregulating cell surface HLA-DR PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables MHC protein binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables ubiquitin protein ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ubiquitin-protein transferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in adaptive immune response IEA
Inferred from Electronic Annotation
more info
  
involved_in antigen processing and presentation of peptide antigen via MHC class II IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in immune response IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein polyubiquitination IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein polyubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in Golgi membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in early endosome membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in endoplasmic reticulum membrane IEA
Inferred from Electronic Annotation
more info
  
located_in endosome IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in late endosome membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in lysosomal membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in lysosome IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in lysosome IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
E3 ubiquitin-protein ligase MARCHF8
Names
E3 ubiquitin-protein ligase MARCH8
RING finger protein 178
RING-type E3 ubiquitin transferase MARCH8
RING-type E3 ubiquitin transferase MARCHF8
c-mir, cellular modulator of immune recognition
cellular modulator of immune recognition (c-MIR)
membrane associated ring finger 8
membrane-associated RING finger protein 8
membrane-associated RING-CH protein VIII
membrane-associated ring finger (C3HC4) 8, E3 ubiquitin protein ligase
NP_001002266.1
NP_001269795.1
NP_001388574.1
NP_001388575.1
NP_659458.2
XP_011537794.1
XP_011537797.1
XP_047280719.1
XP_047280720.1
XP_047280721.1
XP_047280722.1
XP_047280723.1
XP_047280724.1
XP_047280725.1
XP_054221068.1
XP_054221069.1
XP_054221070.1
XP_054221071.1
XP_054221072.1
XP_054221073.1
XP_054221074.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001002266.3 → NP_001002266.1  E3 ubiquitin-protein ligase MARCHF8 isoform a

    See identical proteins and their annotated locations for NP_001002266.1

    Status: VALIDATED

    Source sequence(s)
    AK023636, AK025472, AL833316, BC025394, BM151615
    Consensus CDS
    CCDS7213.1
    UniProtKB/Swiss-Prot
    B2R8E7, H0Y7C6, Q5T0S8, Q5T0T0, Q8TC72
    Related
    ENSP00000379116.2, ENST00000395769.6
    Conserved Domains (1) summary
    smart00744
    Location:79 → 127
    RINGv; The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins

  2. NM_001282866.2 → NP_001269795.1  E3 ubiquitin-protein ligase MARCHF8 isoform b

    See identical proteins and their annotated locations for NP_001269795.1

    Status: VALIDATED

    Source sequence(s)
    AK023636, AK025472, AK307667, AL833316, BM151615
    Consensus CDS
    CCDS60519.1
    UniProtKB/Swiss-Prot
    Q5T0T0
    Related
    ENSP00000411848.2, ENST00000453424.7
    Conserved Domains (1) summary
    smart00744
    Location:361 → 409
    RINGv; The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins

  3. NM_001401645.1 → NP_001388574.1  E3 ubiquitin-protein ligase MARCHF8 isoform b

    Status: VALIDATED

    Source sequence(s)
    AL445201, AL512595, AL731567
    Consensus CDS
    CCDS60519.1

  4. NM_001401646.1 → NP_001388575.1  E3 ubiquitin-protein ligase MARCHF8 isoform b

    Status: VALIDATED

    Source sequence(s)
    AL445201, AL512595, AL731567
    Consensus CDS
    CCDS60519.1

  5. NM_145021.6 → NP_659458.2  E3 ubiquitin-protein ligase MARCHF8 isoform a

    See identical proteins and their annotated locations for NP_659458.2

    Status: VALIDATED

    Source sequence(s)
    AK023636, AK025472, AL445201, AL833316, BC066988, BM151615, HY047787
    Consensus CDS
    CCDS7213.1
    UniProtKB/Swiss-Prot
    B2R8E7, H0Y7C6, Q5T0S8, Q5T0T0, Q8TC72
    Related
    ENSP00000317087.3, ENST00000319836.7
    Conserved Domains (1) summary
    smart00744
    Location:79 → 127
    RINGv; The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000010.11 Reference GRCh38.p14 Primary Assembly

    Range
    45454585..45594907 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_047424768.1 → XP_047280724.1  E3 ubiquitin-protein ligase MARCHF8 isoform X2

    UniProtKB/Swiss-Prot
    B2R8E7, H0Y7C6, Q5T0S8, Q5T0T0, Q8TC72

  2. XM_011539492.4 → XP_011537794.1  E3 ubiquitin-protein ligase MARCHF8 isoform X1

    See identical proteins and their annotated locations for XP_011537794.1

    UniProtKB/Swiss-Prot
    Q5T0T0
    Conserved Domains (1) summary
    smart00744
    Location:361 → 409
    RINGv; The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins

  3. XM_047424765.1 → XP_047280721.1  E3 ubiquitin-protein ligase MARCHF8 isoform X1

  4. XM_047424769.1 → XP_047280725.1  E3 ubiquitin-protein ligase MARCHF8 isoform X2

    UniProtKB/Swiss-Prot
    B2R8E7, H0Y7C6, Q5T0S8, Q5T0T0, Q8TC72

  5. XM_047424764.1 → XP_047280720.1  E3 ubiquitin-protein ligase MARCHF8 isoform X1

  6. XM_047424766.1 → XP_047280722.1  E3 ubiquitin-protein ligase MARCHF8 isoform X1

  7. XM_047424767.1 → XP_047280723.1  E3 ubiquitin-protein ligase MARCHF8 isoform X2

    UniProtKB/Swiss-Prot
    B2R8E7, H0Y7C6, Q5T0S8, Q5T0T0, Q8TC72

  8. XM_047424763.1 → XP_047280719.1  E3 ubiquitin-protein ligase MARCHF8 isoform X1

  9. XM_011539495.2 → XP_011537797.1  E3 ubiquitin-protein ligase MARCHF8 isoform X2

    See identical proteins and their annotated locations for XP_011537797.1

    UniProtKB/Swiss-Prot
    B2R8E7, H0Y7C6, Q5T0S8, Q5T0T0, Q8TC72
    Conserved Domains (1) summary
    smart00744
    Location:79 → 127
    RINGv; The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins

RNA

  1. XR_007061947.1 RNA Sequence

Reference GRCh38.p14 ALT_REF_LOCI_1

Genomic

  1. NW_003315935.1 Reference GRCh38.p14 ALT_REF_LOCI_1

    Range
    295416..305254 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060934.1 Alternate T2T-CHM13v2.0

    Range
    46335573..46475913 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054365098.1 → XP_054221073.1  E3 ubiquitin-protein ligase MARCHF8 isoform X2

  2. XM_054365093.1 → XP_054221068.1  E3 ubiquitin-protein ligase MARCHF8 isoform X1

  3. XM_054365095.1 → XP_054221070.1  E3 ubiquitin-protein ligase MARCHF8 isoform X1

  4. XM_054365099.1 → XP_054221074.1  E3 ubiquitin-protein ligase MARCHF8 isoform X2

  5. XM_054365094.1 → XP_054221069.1  E3 ubiquitin-protein ligase MARCHF8 isoform X1

  6. XM_054365096.1 → XP_054221071.1  E3 ubiquitin-protein ligase MARCHF8 isoform X2

  7. XM_054365097.1 → XP_054221072.1  E3 ubiquitin-protein ligase MARCHF8 isoform X2

RNA

  1. XR_008488176.1 RNA Sequence

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_001002265.1: Suppressed sequence

    Description
    NM_001002265.1: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript and the protein.
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q5T0T0.1 GenPept UniProtKB/Swiss-Prot:Q5T0T0

Gene LinkOut

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