Car9 carbonic anhydrase 9 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Car9provided by MGI
Official Full Name: carbonic anhydrase 9provided by MGI
Primary source: MGI:MGI:2447188
See related: Ensembl:ENSMUSG00000028463 AllianceGenome:MGI:2447188
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ca9; CAIX; MN/CA9
Summary: Predicted to enable carbonate dehydratase activity; molecular function activator activity; and zinc ion binding activity. Acts upstream of or within morphogenesis of an epithelium and secretion. Predicted to be located in basolateral plasma membrane. Predicted to be active in plasma membrane. Is expressed in several structures, including central nervous system; early embryo; genitourinary system; gut; and heart. Human ortholog(s) of this gene implicated in hepatitis B; lung non-small cell carcinoma; and renal cell carcinoma. Orthologous to human CA9 (carbonic anhydrase 9). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in stomach adult (RPKM 45.1), duodenum adult (RPKM 29.1) and 6 other tissues See more
Orthologs: human all
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Genomic context

Location:: 4 A5; 4 23.05 cM

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (43507026..43513729)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (43507026..43513729)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Carbonic Anhydrase IX Suppression Shifts Partial Response to Checkpoint Inhibitors into Complete Tumor Eradication: Model-Based Investigation.
Title: Carbonic Anhydrase IX Suppression Shifts Partial Response to Checkpoint Inhibitors into Complete Tumor Eradication: Model-Based Investigation.
Galectin-1 activates carbonic anhydrase IX and modulates glioma metabolism.
Title: Galectin-1 activates carbonic anhydrase IX and modulates glioma metabolism.
hCA IX and mCA IX are highly similar, and the transcription of both genes is predominantly governed by hypoxia, some attributes of transcriptional regulation are specific for either human or mouse and as such, could result in different tissue expression and data interpretation.
Title: Carbonic Anhydrase IX-Mouse versus Human.
In response to hypoxia, pancreatic ductal adenocarcinoma cells that express activated KRAS increase expression of carbonic anhydrase 9, via stabilization of HIF1A and HIF2A, to regulate pH and glycolysis.
Title: Regulation of pH by Carbonic Anhydrase 9 Mediates Survival of Pancreatic Cancer Cells With Activated KRAS in Response to Hypoxia.
Lack of CAIX results in persistent acid backflux via claudin-18 downregulation, causing loss of parietal cells, hypergastrinaemia and foveolar hyperplasia.
Title: Genetic ablation of carbonic anhydrase IX disrupts gastric barrier function via claudin-18 downregulation and acid backflux.
Authors demonstrate that CAIX associates with MMP14 through potential phosphorylation residues within its intracellular domain, and that CAIX enhances MMP14-mediated collagen degradation by directly contributing hydrogen ions required for MMP14 catalytic activity.
Title: The interactome of metabolic enzyme carbonic anhydrase IX reveals novel roles in tumor cell migration and invadopodia/MMP14-mediated invasion.
Loss of Function Screening Reveals Carbonic Anhydrase IX as a Key Modulator of Tumor Initiating Potential in Primary Pancreatic Tumors.
Title: In Vivo Loss of Function Screening Reveals Carbonic Anhydrase IX as a Key Modulator of Tumor Initiating Potential in Primary Pancreatic Tumors.
constitutive activation of the NF-kappaB pathway in CAIX-depleted cells restored G-CSF secretion
Title: Carbonic anhydrase IX promotes myeloid-derived suppressor cell mobilization and establishment of a metastatic niche by stimulating G-CSF production.
Downregulation of carbonic anhydrase IX promotes Col10a1 expression in chondrocytes.
Title: Downregulation of carbonic anhydrase IX promotes Col10a1 expression in chondrocytes.
CA IX may directly or indirectly play novel functions in brain tissue.
Title: Brain phenotype of carbonic anhydrase IX-deficient mice.

Submit: New GeneRIF Correction See all GeneRIFs (17)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (4)
Targeted (2) 1 citation

General gene information

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Markers

NoName (e-PCR)
- UniSTS:491581

Car9 (e-PCR), detects polymorphism
- UniSTS:546689

Car9 (e-PCR), detects polymorphism
- UniSTS:531396

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables carbonate dehydratase activity	IBA Inferred from Biological aspect of Ancestor more info
enables carbonate dehydratase activity	ISO Inferred from Sequence Orthology more info
enables carbonate dehydratase activity	ISS Inferred from Sequence or Structural Similarity more info
enables molecular function activator activity	IEA Inferred from Electronic Annotation more info
enables molecular function activator activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within morphogenesis of an epithelium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in one-carbon metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to hypoxia	IEA Inferred from Electronic Annotation more info
involved_in response to testosterone	IEA Inferred from Electronic Annotation more info
involved_in response to xenobiotic stimulus	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within secretion	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in basolateral plasma membrane	IEA Inferred from Electronic Annotation more info
located_in basolateral plasma membrane	ISO Inferred from Sequence Orthology more info
located_in microvillus membrane	IEA Inferred from Electronic Annotation more info
located_in nucleolus	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: carbonic anhydrase 9

Names: CA-IX; carbonate dehydratase IX; carbonic anhydrase IX; membrane antigen MN homolog

NP_647466.2

EC 4.2.1.1

XP_006537894.1

EC 4.2.1.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_139305.2 → NP_647466.2 carbonic anhydrase 9 precursor

See identical proteins and their annotated locations for NP_647466.2

Status: VALIDATED

Source sequence(s)

AK136579

Consensus CDS

CCDS18099.1

UniProtKB/Swiss-Prot

Q8K1G1, Q8VDE4, Q8VHB5

UniProtKB/TrEMBL

Q3UUZ9

Related

ENSMUSP00000030183.4, ENSMUST00000030183.10

Conserved Domains (1) summary

cl00012
Location:128 → 370

alpha_CA; Carbonic anhydrase alpha (vertebrate-like) group. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000070.7 Reference GRCm39 C57BL/6J

Range

43507026..43513729

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006537831.4 → XP_006537894.1 carbonic anhydrase 9 isoform X1

Conserved Domains (1) summary

cl00012
Location:50 → 292

alpha_CA; Carbonic anhydrase alpha (vertebrate-like) group. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon ...