Mark4 MAP/microtubule affinity regulating kinase 4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mark4provided by MGI
Official Full Name: MAP/microtubule affinity regulating kinase 4provided by MGI
Primary source: MGI:MGI:1920955
See related: Ensembl:ENSMUSG00000030397 AllianceGenome:MGI:1920955
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Markl1; 2410090P21Rik
Summary: Enables cytoskeletal anchor activity. Involved in positive regulation of NLRP3 inflammasome complex assembly. Is active in microtubule organizing center. Orthologous to human MARK4 (microtubule affinity regulating kinase 4). [provided by Alliance of Genome Resources, Oct 2024]
Expression: Ubiquitous expression in adrenal adult (RPKM 24.4), ovary adult (RPKM 18.8) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 A3; 7 9.78 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (19159027..19193255, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (19425102..19459330, complement)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

MARK4 aggravates cardiac dysfunction in mice with STZ-induced diabetic cardiomyopathy by regulating ACSL4-mediated myocardial lipid metabolism.
Title: MARK4 aggravates cardiac dysfunction in mice with STZ-induced diabetic cardiomyopathy by regulating ACSL4-mediated myocardial lipid metabolism.
MARK4 controls ischaemic heart failure through microtubule detyrosination.
Title: MARK4 controls ischaemic heart failure through microtubule detyrosination.
Mark4 Inhibited the Browning of White Adipose Tissue by Promoting Adipocytes Autophagy in Mice.
Title: Mark4 Inhibited the Browning of White Adipose Tissue by Promoting Adipocytes Autophagy in Mice.
Sub-cortiucal stroke-injured layer 5 cortical neurons up-regulate Mark4, in response to axonal injury.
Title: Ischemic axonal injury up-regulates MARK4 in cortical neurons and primes tau phosphorylation and aggregation.
Inflammasome stimuli can act as polarizing cues to initiate dynamic NLRP3 and MARK4 transport in macrophages.
Title: MARK4 regulates NLRP3 positioning and inflammasome activation through a microtubule-dependent mechanism.
PPARgamma interacted with Mark4 and inhibited the stimulating effect of Mark4 on oxidative stress and inflammation.
Title: Mark4 promotes oxidative stress and inflammation via binding to PPARγ and activating NF-κB pathway in mice adipocytes.
MARK4 is a critical positive regulator of early steps in ciliogenesis.
Title: The microtubule affinity regulating kinase MARK4 promotes axoneme extension during early ciliogenesis.
data indicate a key role of MARK4 in energy metabolism, implicating the kinase as a novel drug target for the treatment of obesity and type 2 diabetes.
Title: Inactivation of MARK4, an AMP-activated protein kinase (AMPK)-related kinase, leads to insulin hypersensitivity and resistance to diet-induced obesity.
The balance between the MARK4 isoforms is carefully guarded during neural differentiation but may be subverted in gliomagenesis.
Title: Differential signature of the centrosomal MARK4 isoforms in glioma.
MARK4 is a functional protein kinase which specifically phosphorylates a cognate peptide substrate for the AMP-kinase family. On overexpression in heterologous cells functional wild-type protein, but not its kinase-dead mutant, decreased cell viability
Title: Identification of regulated genes during permanent focal cerebral ischaemia: characterization of the protein kinase 9b5/MARKL1/MARK4.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Gene trapped (2)
Targeted (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

C79806 (e-PCR)
- UniSTS:159604

RH126744 (e-PCR)
- UniSTS:208086

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables cytoskeletal anchor activity	IDA Inferred from Direct Assay more info	PubMed
enables cytoskeletal anchor activity	ISO Inferred from Sequence Orthology more info
enables gamma-tubulin binding	ISO Inferred from Sequence Orthology more info
enables gamma-tubulin binding	ISS Inferred from Sequence or Structural Similarity more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables microtubule binding	ISO Inferred from Sequence Orthology more info
enables microtubule binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables tau-protein kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables tau-protein kinase activity	ISO Inferred from Sequence Orthology more info
enables tau-protein kinase activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in cilium organization	IEA Inferred from Electronic Annotation more info
involved_in cilium organization	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in microtubule bundle formation	ISS Inferred from Sequence or Structural Similarity more info
involved_in microtubule cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in microtubule cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in microtubule cytoskeleton organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in nervous system development	ISO Inferred from Sequence Orthology more info
involved_in nervous system development	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of NLRP3 inflammasome complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of NLRP3 inflammasome complex assembly	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell cycle	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of cell cycle	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cilium assembly	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cilium assembly	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein localization to centrosome	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of protein localization to centrosome	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in protein phosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of centrosome cycle	IEA Inferred from Electronic Annotation more info
involved_in regulation of centrosome cycle	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in centrosome	ISS Inferred from Sequence or Structural Similarity more info
located_in ciliary basal body	IEA Inferred from Electronic Annotation more info
located_in ciliary basal body	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in dendrite	ISO Inferred from Sequence Orthology more info
located_in dendrite	ISS Inferred from Sequence or Structural Similarity more info
part_of gamma-tubulin complex	IEA Inferred from Electronic Annotation more info
part_of gamma-tubulin complex	ISO Inferred from Sequence Orthology more info
located_in microtubule cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in microtubule cytoskeleton	ISS Inferred from Sequence or Structural Similarity more info
is_active_in microtubule organizing center	IDA Inferred from Direct Assay more info	PubMed
is_active_in microtubule organizing center	ISO Inferred from Sequence Orthology more info
located_in microtubule organizing center	ISO Inferred from Sequence Orthology more info
located_in microtubule organizing center	ISS Inferred from Sequence or Structural Similarity more info
located_in midbody	IEA Inferred from Electronic Annotation more info
located_in midbody	ISO Inferred from Sequence Orthology more info
located_in neuron projection	ISO Inferred from Sequence Orthology more info
located_in neuron projection	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: MAP/microtubule affinity-regulating kinase 4

Names: MAP/microtubule affinity-regulating kinase 4L; MARK4 serine/threonine protein kinase

NP_001355356.1

EC 2.7.11.1

NP_758483.1

EC 2.7.11.1

XP_011248815.1

EC 2.7.11.1

XP_030098296.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001368427.1 → NP_001355356.1 MAP/microtubule affinity-regulating kinase 4 isoform 2

Status: VALIDATED

Source sequence(s)

AC118017, AC149052

Related

ENSMUSP00000159279.2, ENSMUST00000239292.2

Conserved Domains (2) summary

cd14407
Location:328 → 370

UBA_MARK3_4; UBA domain found in MAP/microtubule affinity-regulating kinase MARK3, MARK4, and similar proteins

cd14072
Location:58 → 310

STKc_MARK; Catalytic domain of the Serine/Threonine Kinases, MAP/microtubule affinity-regulating kinases
NM_172279.2 → NP_758483.1 MAP/microtubule affinity-regulating kinase 4 isoform 1

See identical proteins and their annotated locations for NP_758483.1

Status: VALIDATED

Source sequence(s)

AC118017, AC149052

Consensus CDS

CCDS20903.1

UniProtKB/Swiss-Prot

Q80T81, Q8CIP4

Related

ENSMUSP00000082862.6, ENSMUST00000085715.7

Conserved Domains (4) summary

cd12197
Location:654 → 752

MARK4_C; C-terminal, kinase associated domain 1 (KA1), a phospholipid binding domain, of microtubule affinity-regulating kinase 4

smart00220
Location:59 → 310

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14407
Location:328 → 370

UBA_MARK3_4; UBA domain found in MAP/microtubule affinity-regulating kinase MARK3, MARK4, and similar proteins

cd14072
Location:58 → 310

STKc_MARK; Catalytic domain of the Serine/Threonine Kinases, MAP/microtubule affinity-regulating kinases

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

19159027..19193255 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011250513.4 → XP_011248815.1 MAP/microtubule affinity-regulating kinase 4 isoform X1

Conserved Domains (3) summary

cd12197
Location:545 → 643

MARK4_C; C-terminal, kinase associated domain 1 (KA1), a phospholipid binding domain, of microtubule affinity-regulating kinase 4

cd14407
Location:219 → 261

UBA_MARK3_4; UBA domain found in MAP/microtubule affinity-regulating kinase MARK3, MARK4, and similar proteins

cl21453
Location:1 → 201

PKc_like; Protein Kinases, catalytic domain
XM_030242436.1 → XP_030098296.1 MAP/microtubule affinity-regulating kinase 4 isoform X2

Conserved Domains (2) summary

cd14407
Location:219 → 261

UBA_MARK3_4; UBA domain found in MAP/microtubule affinity-regulating kinase MARK3, MARK4, and similar proteins

cl21453
Location:1 → 201

PKc_like; Protein Kinases, catalytic domain