ALOX15B arachidonate 15-lipoxygenase type B [Homo sapiens (human)] - Gene

Summary

Official Symbol: ALOX15Bprovided by HGNC
Official Full Name: arachidonate 15-lipoxygenase type Bprovided by HGNC
Primary source: HGNC:HGNC:434
See related: Ensembl:ENSG00000179593 MIM:603697; AllianceGenome:HGNC:434
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: 15-LOX-2
Summary: This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Expression: Biased expression in prostate (RPKM 42.3), esophagus (RPKM 13.5) and 2 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 17p13.1

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	17	NC_000017.11 (8039059..8049134)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	17	NC_060941.1 (7944477..7954568)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	17	NC_000017.10 (7942377..7952452)

Chromosome 17 - NC_000017.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

ALOX15B controls macrophage cholesterol homeostasis via lipid peroxidation, ERK1/2 and SREBP2.
Title: ALOX15B controls macrophage cholesterol homeostasis via lipid peroxidation, ERK1/2 and SREBP2.
15-lipoxygenase and cyclooxygenase expression profile and their related modulators in COVID-19 infection.
Title: 15-lipoxygenase and cyclooxygenase expression profile and their related modulators in COVID-19 infection.
Low 15-LOX-2 expression is associated with lung carcinogenesis.
Title: Ambient fine particulate matter inhibits 15-lipoxygenases to promote lung carcinogenesis.
ALOX15B expression and up-regulation in the functional and non-functional pituitary adenomas.
Title: Up-regulation of 15-lipoxygenase enzymes and products in functional and non-functional pituitary adenomas.
we identified a novel role for ALOX15B, and to a lesser extent ALOX15, in cholesterol homeostasis and CCL17 production in human macrophages.
Title: A Novel Function for 15-Lipoxygenases in Cholesterol Homeostasis and CCL17 Production in Human Macrophages.
Results demonstrate the expression of ALOX15 and ALOX15B in failing as well as in donor hearts. ALOX15/B signaling may play an important role in heart disease, including heart failure. Isolated fibroblasts from all four chambers of the heart expressed ALOX15 as well as ALOX15B, and gene expression levels were further increased under hypoxia.
Title: Hypoxic cardiac fibroblasts from failing human hearts decrease cardiomyocyte beating frequency in an ALOX15 dependent manner.
Ca(2+) binding induces major structural changes in the 15-lipoxygenase-2 PLAT domain.
Title: Structural Dynamics of 15-Lipoxygenase-2 via Hydrogen-Deuterium Exchange.
15-LOX-2 is distributed at the plasma membrane when cells are stimulated by the addition Ca(2+) ionophore and that cellular localization is dependent upon the presence of a putative membrane insertion loop.
Title: Membrane-dependent Activities of Human 15-LOX-2 and Its Murine Counterpart: IMPLICATIONS FOR MURINE MODELS OF ATHEROSCLEROSIS.
these results demonstrate the strict regiospecificity of h15-LOX-2 that circumscribes its role in transcellular synthesis.
Title: Strict Regiospecificity of Human Epithelial 15-Lipoxygenase-2 Delineates Its Transcellular Synthesis Potential.
This study combined molecular dynamics simulations, QM/MM calculations, and umbrella sampling free energy simulations to study the hydrogen atom abstraction from arachidonic acid catalyzed by the human enzyme 15-lipoxygenase-2.
Title: Understanding the Mechanism of the Hydrogen Abstraction from Arachidonic Acid Catalyzed by the Human Enzyme 15-Lipoxygenase-2. A Quantum Mechanics/Molecular Mechanics Free Energy Simulation.

Submit: New GeneRIF Correction See all GeneRIFs (38)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Parent-of-origin-specific allelic associations among 106 genomic loci for age at menarche. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See Variation Viewer (GRCh37.p13)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D17S561 (e-PCR)
- UniSTS:151874

SHGC-146014 (e-PCR)
- UniSTS:174940

PMC21017P1 (e-PCR)
- UniSTS:272008

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables arachidonate 15-lipoxygenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables arachidonate 15-lipoxygenase activity	IDA Inferred from Direct Assay more info	PubMed
enables arachidonate 8(S)-lipoxygenase activity	IEA Inferred from Electronic Annotation more info
enables calcium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables iron ion binding	IDA Inferred from Direct Assay more info	PubMed
enables linoleate 13S-lipoxygenase activity	IDA Inferred from Direct Assay more info	PubMed
enables linoleate 9S-lipoxygenase activity	IEA Inferred from Electronic Annotation more info
enables lipid binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in apoptotic process	TAS Traceable Author Statement more info	PubMed
involved_in arachidonate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in arachidonate metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in cannabinoid biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in endocannabinoid signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in hepoxilin biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in hepoxilin biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in linoleic acid metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in lipid metabolic process	TAS Traceable Author Statement more info	PubMed
involved_in lipid oxidation	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipoxin A4 biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in lipoxygenase pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of cell cycle	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of cell migration	TAS Traceable Author Statement more info	PubMed
involved_in negative regulation of cell population proliferation	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of cell population proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of growth	IDA Inferred from Direct Assay more info	PubMed
involved_in phospholipid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of chemokine production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of keratinocyte differentiation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of macrophage derived foam cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of peroxisome proliferator activated receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in prostate gland development	NAS Non-traceable Author Statement more info	PubMed
involved_in regulation of epithelial cell differentiation	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in adherens junction	IDA Inferred from Direct Assay more info	PubMed
located_in cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
located_in extracellular exosome	HDA more info	PubMed
located_in focal adhesion	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
NOT located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: polyunsaturated fatty acid lipoxygenase ALOX15B

Names: 15-LOX-B; 15S-lipoxygenase; arachidonate 15-lipoxygenase 2; arachidonate 15-lipoxygenase type II; arachidonate 15-lipoxygenase, second type; arachidonate omega(6) lipoxygenase; linoleate 13-lipoxygenase 15-LOb

NP_001034219.1

EC 1.13.11.33

NP_001034220.1

EC 1.13.11.33

NP_001132.2

EC 1.13.11.33

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_029482.1 RefSeqGene

Range

5020..15095

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001039130.2 → NP_001034219.1 polyunsaturated fatty acid lipoxygenase ALOX15B isoform a

Status: REVIEWED

Description

Transcript Variant: This variant (a) lacks an exon in the 3' coding region but maintains the reading frame, compared to variant d. The encoded isoform (a) is shorter than isoform d.

Source sequence(s)

AF468052, BC063647, BP327830, CA307212, CA405703

Consensus CDS

CCDS32558.1

UniProtKB/TrEMBL

A8K9R4

Related

ENSP00000369520.2, ENST00000380173.6

Conserved Domains (2) summary

cd01753
Location:2 → 121

PLAT_LOX; PLAT domain of 12/15-lipoxygenase. As a unique subfamily of the mammalian lipoxygenases, they catalyze enzymatic lipid peroxidation in complex biological structures via direct dioxygenation of phospholipids and cholesterol esters of biomembranes and ...

pfam00305
Location:232 → 638

Lipoxygenase; Lipoxygenase
NM_001039131.2 → NP_001034220.1 polyunsaturated fatty acid lipoxygenase ALOX15B isoform b

Status: REVIEWED

Description

Transcript Variant: This variant (b) has multiple differences in the coding region but maintains the reading frame, compared to variant d. The encoded isoform (b) is shorter than isoform d.

Source sequence(s)

AF468053, BC063647, BP327830, CA307212, CA405703

Consensus CDS

CCDS32559.1

UniProtKB/TrEMBL

I3L1D5

Related

ENSP00000460332.2, ENST00000573359.1

Conserved Domains (2) summary

cd01753
Location:2 → 121

PLAT_LOX; PLAT domain of 12/15-lipoxygenase. As a unique subfamily of the mammalian lipoxygenases, they catalyze enzymatic lipid peroxidation in complex biological structures via direct dioxygenation of phospholipids and cholesterol esters of biomembranes and ...

pfam00305
Location:232 → 593

Lipoxygenase; Lipoxygenase
NM_001141.3 → NP_001132.2 polyunsaturated fatty acid lipoxygenase ALOX15B isoform d

See identical proteins and their annotated locations for NP_001132.2

Status: REVIEWED

Description

Transcript Variant: This variant (d) represents the longest transcript and encodes the longest isoform (d).

Source sequence(s)

AF468051, BC063647, BP327830, CA307212, CA405703

Consensus CDS

CCDS11128.1

UniProtKB/Swiss-Prot

D3DTR2, O15296, Q8IYQ2, Q8TEV3, Q8TEV4, Q8TEV5, Q8TEV6, Q9UKM4

UniProtKB/TrEMBL

A8K9R4

Related

ENSP00000369530.4, ENST00000380183.9

Conserved Domains (2) summary

cd01753
Location:2 → 121

PLAT_LOX; PLAT domain of 12/15-lipoxygenase. As a unique subfamily of the mammalian lipoxygenases, they catalyze enzymatic lipid peroxidation in complex biological structures via direct dioxygenation of phospholipids and cholesterol esters of biomembranes and ...

pfam00305
Location:232 → 667

Lipoxygenase; Lipoxygenase