sap1 switch-activating protein Sap1 [Schizosaccharomyces pombe (fission yeast)] - Gene

Summary

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Official Symbol: sap1
Official Full Name: switch-activating protein Sap1
Primary source: PomBase:SPCC1672.02c
Locus tag: SPOM_SPCC1672.02C
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Schizosaccharomyces pombe (strain: 972h-)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
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Genomic context

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See sap1 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 1

Sequence:: Chromosome: III; NC_003421.2 (561576..563766, complement)

Chromosome III - NC_003421.2 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003421.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Crystal structure results identified a dimerization interface between Sap1 monomers that predicted to be engaged in the direct repeat binding mode. Interestingly, mutations in Sap1 which rescue the CENP-B deletion phenotype lie at or near this dimerization interface.
Title: Structure of the replication regulator Sap1 reveals functionally important interfaces.
Sap1 is a replication-initiation factor that directly participates in the assembly of the pre-RC. DNA-replication origins in fission yeast are defined by possessing two essential elements with one bound by ORC and the other by Sap1.
Title: Sap1 is a replication-initiation factor essential for the assembly of pre-replicative complex in the fission yeast Schizosaccharomyces pombe.
This study shows that insertion of the fission yeast LTR retrotransposon Tf1 is guided by the DNA binding protein Sap1 and that the efficiency and location of the targeting depend on the activity of Sap1 as a replication fork barrier.
Title: Arrested replication forks guide retrotransposon integration.
Sap1 is required for replication fork arrest at the rRNA genes of fission yeast
Title: The mating type switch-activating protein Sap1 Is required for replication fork arrest at the rRNA genes of fission yeast.
Sap1 is required to recruit the replication fork protection complex to chromatin, thereby contributing to the activation of the replication checkpoint and the stabilization of replication forks.
Title: Sap1 promotes the association of the replication fork protection complex with chromatin and is involved in the replication checkpoint in Schizosaccharomyces pombe.
Results reveal that Sap1-DNA affinity alone is insufficient to account for fork arrest and suggest that Sap1 binding-induced structural changes may result in formation of a competent fork-blocking complex.
Title: Molecular architecture of a eukaryotic DNA replication terminus-terminator protein complex.
Sap1p binds the rDNA fork barrier Ter1 to cause polar replication fork arrest at this site but not at SAS1.
Title: Sap1p binds to Ter1 at the ribosomal DNA of Schizosaccharomyces pombe and causes polar replication fork arrest.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by PomBase

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding, bending	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables rDNA spacer replication fork barrier binding	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific double-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in gene conversion at mating-type locus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic pre-replicative complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in replication fork arrest at rDNA repeats	IDA Inferred from Direct Assay more info	PubMed
involved_in replication fork arrest at rDNA repeats	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in site-specific DNA replication termination	IDA Inferred from Direct Assay more info	PubMed